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Fluorescence-labeled magnetic kaempferol microsphere system and preparation method thereof

A technology of fluorescent labeling and kaempferol, which is applied in the field of nanomaterials, can solve the problems of high synthesis cost and no research or report on the drug-loading system of fluorescent magnetic kaempferol microspheres, and achieve mild experimental conditions, simple preparation methods, and good inhibition The effect of action

Active Publication Date: 2015-11-18
南京美材科技有限公司
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Problems solved by technology

However, the synthesis cost of this method is high, and the controlled release performance analysis of the system loaded with antineoplastic drug doxorubicin has not been carried out
[0008] At present, there is no research or report on the drug-loading system of fluorescent magnetic kaempferol microspheres

Method used

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  • Fluorescence-labeled magnetic kaempferol microsphere system and preparation method thereof
  • Fluorescence-labeled magnetic kaempferol microsphere system and preparation method thereof
  • Fluorescence-labeled magnetic kaempferol microsphere system and preparation method thereof

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Embodiment

[0043] Add 5mg kaempferol to 2g oil-based modified Fe 3 o 4 After forming a mixed solution in the magnetic fluid, slowly drop the mixed solution into 50 mL of 2% bovine serum albumin solution with a syringe, and stir to form a uniform emulsion. Next, the emulsion was ultrasonicated for 15 minutes at a power of 300W, and after the reaction, the product was washed and dried to prepare magnetic kaempferol microspheres, which were sealed and stored for future use.

[0044] Aminated graphene quantum dots are synthesized by hydrothermal method, see literature: WangL, WangYL, XuT, etal, Naturecommunications, 2014, 5:5357. First weigh 2 g of pyrene (purity>98%), measure 160 mL of concentrated nitric acid, mix them and put them into a round bottom flask. Under the condition of a constant temperature water bath, the mixture was heated to 80° C. and stirred by electric motor for 12 h to obtain a yellow solution, which was cooled to room temperature. Then measure 1 L of deionized water...

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Abstract

The invention discloses a fluorescence-labeled magnetic kaempferol microsphere system and a preparation method thereof. The preparation method comprises the following steps: (1) firstly, dissolving a hydrophobic drug, namely hydrophobic drug, in an oil-base modified magnetic fluid, uniformly mixing, dropping the dissolved kaempferol to a proper amount of a bovine serum albumin solution by virtue of an injector, and synthesizing a magnetic kaempferol protein microsphere through an ultrasonic chemical method; (2) then, connecting to self-made graphene quantum dots through an amino carboxylic reaction so as to obtain a fluorescent and magnetic dual-functional kaempferol microsphere; and (3) co-cultivating the a fluorescent and magnetic dual-functional kaempferol microsphere with human Hela cells, and detecting cytotoxicity by virtue of an MTT method. The fluorescence-labeled magnetic kaempferol microsphere disclosed by the invention is low in preparation cost and high in drug loading rate; the microsphere not only has the superparamagnetism of magnetic particles but also reserves the excellent fluorescence performance of the graphene quantum dots; and the microsphere can achieve an excellent effect on medicines for tumor targeted therapy.

Description

technical field [0001] The invention relates to the field of nanomaterials and biotechnology, in particular to a fluorescently labeled magnetic kaempferol microsphere drug-loading system and a preparation method thereof. Background technique [0002] Due to their magnetic responsiveness and different surface functions, magnetic microspheres are increasingly used in targeted drug loading, cell separation, immobilized enzymes and other fields. With the advancement of medical technology and the improvement of requirements for biomedical materials, magnetic microspheres with both magnetic properties and excellent fluorescent labeling functions have attracted more and more attention. [0003] Kaempferol (KAEmpferol, KAE) is derived from the rhizome of Zingiberaceae kaempferum, a natural polyphenolic flavonoid compound, which has high medical value in anticancer, antibacterial, anti-inflammatory, anti-epileptic, and cough-relieving. However, kaempferol is a hydrophobic drug with ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00A61K31/352A61K47/42A61P35/00
Inventor 张小娟郝凌云徐梦张志颖周宇琳周啸刚
Owner 南京美材科技有限公司
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