Emulsion stabilizer for polyethylene glycol/dextran two-phase aqueous system and preparation method thereof

Abstract

The invention discloses a polyethylene glycol / dextran two-phase system emulsion stabilizer and a preparation method thereof, wherein methoxypolyethylene glycol aldehyde (mPEG-CHO) is added to an aqueous solution of bovine serum albumin and fully stirred until Dissolve, wherein bovine serum albumin:mPEG-CHO=1:10~1:13 (molar ratio). Then add a certain amount of dilute hydrochloric acid to the above aqueous solution to adjust the pH value to be slightly higher than the isoelectric point of bovine serum albumin. After fully reacting for 20 hours at a constant temperature of 0-4°C, the target product can be obtained after precipitation and drying. The method of the invention is simple, the preparation condition is mild, the control is easy, and the side reaction is less. Because the surface of the invented product has moderate wettability with both polyethylene glycol and dextran phases, it is adsorbed on the two-phase interface and achieves the effect of stabilizing the two-phase emulsion by changing the interfacial tension, and retains to a large extent The biological activity of bovine serum albumin can realize the loading of biologically active substances in the two-phase emulsion, and has a good application prospect in the fields of food, cosmetics and drug controllable release.

Description

technical field [0001] The invention relates to the field of fine chemical industry, especially the preparation of two-phase emulsion stabilizer. Background technique [0002] The traditional emulsion preparation process needs to add a large amount of surfactants and organic solvents, so when the emulsion product functions, a large amount of surfactants and organic solvents will be discharged into the environment, causing environmental pollution. Many industries have begun to call for the emergence of new emulsions with short dissolution time, convenient use, and environmental protection. The emergence of water-in-water emulsions solves the above problems. The preparation process of water-in-water emulsions does not need to add any organic solvents, and has good solubility and dispersibility. , easy to use, green and environmentally friendly, and high biocompatibility. However, water-in-water emulsions have disadvantages such as difficult milk formation and poor stability, ...

AI Technical Summary

Problems solved by technology

However, the protein particles used to stabilize aqueous two-phase emulsions are not natural proteins, but prepared by heating protein solutions, and the protein particles have been denatured and lost their biological activity; triblock copolymers used to stabilize aqueous two-phase emulsions It is produced by the copolymerization of three different polymers. The production cost of the tri-block copolymer is high, the preparation process is relatively complicated, and the block does not contain biologically active substances

Since the research in this field is in its infancy, there are too few methods for stabilizing two-phase emulsions, narrow application ranges, and products that can impart biological activity to two-phase emulsion stabilizers have not been reported, so the market is in urgent need of a new type of two-phase emulsion. Phase emulsion emulsifier, which can not only stabilize water-in-water emulsion, but also expand the application of water-in-water emulsion in loading biological active substances