Pickle containing mixed lactic acid bacteria and making method thereof

A technology of lactic acid bacteria and kimchi, applied in the direction of lactobacillus, food preparation, bacteria used in food preparation, etc., can solve the problems of reducing nitrite content in fermented vegetables, achieve stable product quality, simple production process, and ensure consistency Effect

Inactive Publication Date: 2015-11-25
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, many scholars have used artificial inoculation of lactic acid bacteria to shorten the fermentation period of vegetable products, improve the quality of fermented vegetables, and significantly reduce the content of nitrite in fermented vegetables. Reports on the Application of Kimchi in Food
[0004] The patent application number is 94111749.9. The Chinese invention patent discloses a process for producing kimchi using purebred lactic acid bacteria. It takes about 15 days to mature at room temperature, and it needs to add up to 13.5-15.0% salt. The process has a long growth cycle and contains high Level of salt, with the continuous improvement of people's living standards, the requirements for food quality are getting higher and higher. Fermented kimchi with low salinity and stronger health care functions is more beneficial to people's health, so low salt and helps to degrade the human body Cholesterol fermented kimchi has become the development direction of fermented vegetables at home and abroad

Method used

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  • Pickle containing mixed lactic acid bacteria and making method thereof
  • Pickle containing mixed lactic acid bacteria and making method thereof
  • Pickle containing mixed lactic acid bacteria and making method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] (1) Place the freeze-dried powders of Lactobacillus DMDL9010 and Lactobacillus casei subsp. The number of bacteria reached 2.0×10 8 CFU / mL; said Lactobacillus casei subsp. rhamnosus was purchased from China Industrial Microorganism Culture Collection Center, numbered CICC6013, named Lactobacillus caseisubsp.rhamnosus6013 (abbreviated as LCR6013).

[0031] (2) With a volume percentage of 5%, the seed liquids of Lactobacillus DMDL9010 and Lactobacillus casei subsp. The number of live bacteria reached 8.2×10 8 CFU / mL, 7.9×10 8 CFU / mL; the formula of the working fermentation medium is: 85 parts of fresh mustard juice, 5 parts of tomato juice, 0.5 parts of vitamin C, 1.5 parts of NaCl, distilled water to 100 parts, cooled after sterilization for later use, namely For the working fermentation medium.

[0032] (3) Select kohlrabi, cabbage, carrots and mustard greens that are fresh, moderately mature, free from borers, free from rot, and thick and firm.

[0033] (4) Remove...

Embodiment 2

[0056] (1) The freeze-dried powders of Lactobacillus DMDL9010 and Lactobacillus casei subsp. The number of bacteria reached 5.0×10 8 CFU / mL;

[0057] (2) With a volume percentage of 10%, the seed liquids of Lactobacillus DMDL9010 and Lactobacillus casei subsp. The number of viable bacteria reached 2.5×10 8 CFU / mL, 5.5×10 8 CFU / mL; the formula of the working fermentation medium is: 85 parts of fresh cabbage juice, 5 parts of tomato juice, 3.0 parts of vitamin C, 1.5 parts of NaCl, distilled water to 100 parts, after sterilization, cool and set aside, that is For the working fermentation medium.

[0058] (3) Choose cabbage, cucumber, lettuce that are fresh, moderately ripe, free from borers, free from rot, and thick and firm;

[0059] (4) Remove the old, rotten and inedible parts, and wash the silt on the surface with clean water, drain the water on the surface, cut into appropriate sizes and blanch with hot water;

[0060] (5) According to the weight of 30 parts of vegeta...

Embodiment 3

[0064] (1) The freeze-dried powders of Lactobacillus DMDL9010 and Lactobacillus casei subsp. The number of bacteria reached 4.5×10 8 CFU / mL;

[0065] (2) With a volume percentage of 1%, the seed liquids of Lactobacillus DMDL9010 and Lactobacillus casei subsp. The number of viable bacteria reached 5.0×10 8 CFU / mL, 4.2×10 8 CFU / mL; the formula of the working fermentation medium is: 70 parts of fresh mustard juice, 5 parts of tomato juice, 5 parts of carrot juice, 1.5 parts of vitamin C, 3.0 parts of NaCl, distilled water to 100 parts, after sterilization After cooling for later use, it is the working fermentation medium.

[0066] (3) Select potherb mustard, peppers and mustard greens that are fresh, moderately mature, free from moths, rotten, and thick and firm;

[0067] (4) Remove the old, rotten and inedible parts, and wash the sediment on the surface with clean water, drain the water on the surface, cut into appropriate sizes and blanch with hot water;

[0068](5) Accor...

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Abstract

The invention discloses pickle containing mixed lactic acid bacteria and a making method thereof. The mixed lactic acid bacteria contain Lactobacillus sp. DMDL 9010 and Lactobacillus casei subsp. Rhamnosus, the lactic acid bacteria are preserved in the Chinese common microbe bacterial preservation administration center on August 19, 2011, and the preservation serial number is CGMCC No.5172. The making method includes the steps that fresh vegetables are added into the mixed lactic acid bacteria to be subjected to anaerobic fermentation at the temperature of 20 DEG C to 40 DEG C 60 hours to 72 hours later, the mixed anaerobic fermentation is placed at the normal temperature for six months, the viable count of the lactic acid bacteria can reach 105 cfu / g to 106 cfu / g, and the salt content is lower than 3.0%. The made pickle is nearly free of nitrite, short in production cycle and stable in product quality, contains active probiotics and is instant food.

Description

technical field [0001] The invention belongs to the technical field of food, and specifically refers to a pickle containing mixed lactic acid bacteria and a preparation method thereof. Background technique [0002] Probiotics are live microbial food ingredients that benefit human health. Probiotics are usually lactic acid bacteria or bifidobacteria, mainly of the Lactobacillus genus, but also include some other enterococci, propionibacterium and Clostridium. [0003] On the one hand, because fresh vegetables contain a lot of nitrates, some harmful bacteria in pickles will be converted into nitrites under the action of nitrate reductase during the fermentation process. At the same time, phenolic substances and vitamin C in vegetables Substances such as nitrate will also have a certain reducing effect on nitrate, and after the excessive nitrite in pickles is eaten into the stomach, it will react with the secondary amine of protein decomposition products under the action of hy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/218A23L1/03A23L1/302A23L19/20A23L29/00A23L33/15
CPCA23V2002/00A23V2400/125A23V2250/708
Inventor 刘冬梅黄娟郭均杨丹霞黄智斌韩欣杨慧宁吴晖
Owner SOUTH CHINA UNIV OF TECH
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