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Application of matrix metallo-proteinase inhibitor in resisting nuclear radiation

A matrix metal and protease technology, applied in the field of anti-nuclear radiation, can solve the problems of many side effects and high toxicity

Inactive Publication Date: 2015-11-25
ACADEMY OF MILITARY MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the drugs used clinically to resist radiation damage mainly include WR-2721 (amifostine), superoxide dismutase, etc., but their high toxicity and many side effects limit their application.

Method used

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  • Application of matrix metallo-proteinase inhibitor in resisting nuclear radiation
  • Application of matrix metallo-proteinase inhibitor in resisting nuclear radiation
  • Application of matrix metallo-proteinase inhibitor in resisting nuclear radiation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1. The effect of heavy ion radiation on the development of zebrafish embryos

[0049] The inventor conducted experimental research with heavy ions (carbon particles) commonly used in nuclear radiation experiments, irradiated zebrafish embryos with different doses of heavy ions, and then observed them to calculate the mortality rate, deformity rate and deformity phenotype. The specific experiment is carried out according to the following steps: fish are paired in the evening of the first two days of the experiment, and the separator is removed in the morning of the second day, and the male and female fish rear-end and are fertilized. Collect the same batch of fertilized eggs. Wash the fertilized eggs of zebrafish 3-4 times with E3 culture medium to wash away the excrement, food and other impurities of the zebrafish, and then place the fertilized eggs in a constant temperature incubator at 28°C for incubation. During the incubation process, find out in time to re...

Embodiment 2

[0050] Example 2 Discovery of Heavy Ion Radiation Susceptibility Genes

[0051] Put the zebrafish embryos from the same parent 24h after fertilization into a 6-well plate, put 20-30 embryos in each well, divide them into 12 wells, and divide them into 9 radiation groups and 3 control groups (non-irradiation group) The irradiation group was irradiated with 9Gy, 12Gy, 15Gy of 12C6+ protons, and continued to culture and hatch. After 7 days of irradiation, the zebrafish in the irradiation group and the normal control group were blotted dry and placed in cryopreservation tubes and frozen in liquid nitrogen. , for subsequent gene chip detection.

[0052] Chip synthesis, signal acquisition, quality control, and analysis were all performed by Beijing National Engineering Research Center of Boao Biological Co., Ltd. All the data analysis adopts Boao company's MAS3.0 system. According to the analysis results of the gene chip, genes with large differences were found, and primers were d...

Embodiment 3

[0059] The radiation protection effect of embodiment 3 matrix metalloproteinase inhibitors

[0060] Zebrafish embryos were given matrix metalloproteinase inhibitors and then subjected to heavy ion radiation, and the mortality, hatchability and deformity phenotypes were calculated to obtain the radiation resistance effect. The most representative drug of matrix metalloproteinase inhibitors is ilomastat (Ilomastat), and the inventor conducted a series of studies using him as an experimental drug. Specific experimental steps: fish were bred 2 nights before the heavy ion irradiation test, and the next morning, the board was drawn, and the male and female fish were chased and fertilized. Zebrafish fertilized eggs were collected, washed, and placed in an incubator at 28°C for incubation. Injection was performed after 24hpf. Each embryo was injected with 0.5mg / ml matrix metalloproteinase inhibitor solution 10nl, and normal saline was injected as the control group. To determine the ...

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Abstract

The invention relates to an application of a matrix metallo-proteinase inhibitor in resisting nuclear radiation, and specifically, the invention relates to biological susceptibility gene mmp9 of nuclear radiation, and further relates to a function for preventing nuclear radiation of a matrix metallo-proteinase inhibitor. The matrix metallo-proteinase inhibitor can be developed into dosage forms including subcutaneous injection, intramuscular injection and intravenous injection, and can be prepared into dosage forms including buccal tablets, nasal sprays, rectum administration agents with transmembrane carriers including transduction peptide and nanometer for prevention and treatment of nuclear radiation, and in addition, the matrix metallo-proteinase inhibitor also can be used for treatment on diseases including tumour.

Description

technical field [0001] The invention relates to the development of nuclear radiation susceptibility genes and matrix metalloproteinase inhibitors as drugs for preventing and controlling nuclear radiation and the application of matrix metalloproteinase inhibitors in resisting nuclear radiation. Background technique [0002] In recent years, facilities for the peaceful use of nuclear energy have been widely promoted, and nuclear leakage accidents have occurred frequently. Nuclear weapons have always existed as a military threat, and nuclear terrorist incidents using dirty bombs have also brought greater terrorist threats to people's lives. While strictly investigating and preventing problems before they happen, we must also be prepared to develop anti-radiation drugs for prevention and treatment, so as to minimize losses. [0003] With the rapid development of aerospace science and technology, manned spacecraft have been launched into space one after another, and exploration ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/55A61P39/00G01N33/48
Inventor 赵宝全查晓丹李小满李前黄春倩孙曼霁
Owner ACADEMY OF MILITARY MEDICAL SCI
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