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A high-yield l-amino acid oxidase strain and its application

An amino acid and oxidase technology, which is applied in the direction of enzymes, bacteria, and microorganism-based methods, can solve problems such as harsh reaction conditions, large equipment corrosion, and complicated separation, and achieve stable product quality, energy saving in the process, and good biological safety. Effect

Active Publication Date: 2018-11-16
NANJING TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods have harsh reaction conditions, high energy consumption, large equipment corrosion, low efficiency, and complicated separation.

Method used

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  • A high-yield l-amino acid oxidase strain and its application
  • A high-yield l-amino acid oxidase strain and its application
  • A high-yield l-amino acid oxidase strain and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] In this example:

[0041] 1) The complete medium consists of glucose 2 g / L, peptone 10 g / L, yeast powder 5 g / L, NaCl 5 g / L, pH 7.0, solid medium plus agar 15 g / L;

[0042] 2) The basic medium consists of glucose 20 g / L, sodium citrate 5 g / L, K 2 HPO 4 7 g / L, KH 2 PO 4 7 g / L, MgSO 4 0.1 g / L, (NH 4 ) 2 SO 4 2 g / L, pH 7.0, solid medium plus agar 15 g / L;

[0043] 3) The screening medium is 40 μg / mL lysine added to the basic medium;

[0044]4) LB slant medium / seed medium is peptone 10 g / L, yeast powder 5 g / L, NaCl 5 g / L, pH7.0, solid medium plus agar 15 g / L;

[0045] 5) The fermentation medium is peptone 50g / L, (NH 4 ) 2 SO 4 5.5g / L 、NaH 2 PO 4 2H 2 O 4.2 g / L, K 2 HPO 4 ·3H 2 O 8.7 g / L, (NH 4 ) 2 SO 4 5.5 g / L, MgSO 4 ·7H 2 O 2.5 g / L or MgSO 4 3.7 g / L, ammonia water and 2 mol / L hydrochloric acid were used to control the pH of the fermentation broth during the fermentation process.

[0046] Such as figure 1 As shown, the specific steps for obt...

Embodiment 2

[0060] This example illustrates the physiological and biochemical characteristics of Escherichia coli MZ505

[0061] The morphological and physiological and biochemical characteristics of Escherichia coli MZ505 obtained in the above-mentioned embodiment 1 are as follows:

[0062] Colony color: milky white to yellowish.

[0063] Bacteria morphology: round colony with metallic luster.

[0064] Gram stain: negative.

[0065] Colony size: 0.2-0.8 x 1-4 μm.

[0066] Aerobic mode: aerobic growth.

[0067] Suitable growth temperature: about 37 ℃.

[0068] Suitable growth pH: around 7.0.

[0069] The genetic stability test of Escherichia coli MZ505 in Example 1, and the strain subculture and fermentation experiments are shown in Table 1.

[0070] Table 1 is the genetic stability test of Escherichia coli MZ505

[0071]

[0072] It can be seen from experiments that after 8 passages, the content of L-amino acid oxidase in the genetically engineered bacteria is stable, and has g...

Embodiment 3

[0074] The activated seed liquid after the overnight culture of the embodiment was inserted into the shake flask culture medium, and the culture conditions were as follows: temperature 37 ° C, stirring speed 200 rpm, inoculum size was 1% by volume, Kana Resistance is 0.2% by volume. When the cell mass of the strain grew to 0.246g / L, 0.6 mM IPTG was added to induce expression at 20°C, and cultured at a stirring speed of 200 rpm for 9 h; the crude enzyme solution of L-amino acid oxidase was obtained by ultrasonic crushing.

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Abstract

The invention relates to a strain with a high L-amino acid oxidase yield. The classification name of the strain is escherichia coli MZ505. The invention further provides a screening method of the strain, a method for producing L-amino acid oxidase with the strain, and application of L-amino acid oxidase produced by the strain to production of 5-aminovaleric acid.

Description

technical field [0001] The invention relates to the fields of biotechnology and organic synthesis, in particular to a high-production L-amino acid oxidase strain, a method for producing L-amino acid oxidase by using the strain and the application of the enzyme in catalyzing the synthesis of 5-aminovaleric acid. Background technique [0002] 5-aminovaleric acid, the English name is 5-Aminovaleric acid , as a commonly used biochemical reagent, it has excellent performance and a wide range of applications. As an important precursor for the synthesis of nylon 5, glutaric acid, 5-hydroxyvaleric acid, 1,5-pentanediol and its derivatives, and glutaric anhydride, glutaric anhydride is a chemical and biopharmaceutical intermediate, so it is used in The fields of textile industry, machinery industry, fine chemical synthesis and medicine have broad prospects. The traditional methods for preparing 5-aminovaleric acid are all synthesized by chemical method, generally obtained by hydrol...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N9/06C12P13/00C12R1/19
Inventor 陈可泉马金莲应晗笑王璟欧阳平凯
Owner NANJING TECH UNIV
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