Serum-free stem cell culture medium and application thereof

Abstract

The invention provides a serum-free stem cell culture medium which comprises a basal culture medium and supplement factors. The recruitment factors include sodium arsenite, transferrin, bovine serum albumin, insulin, fibronectin and hydrocortisone. Compared with serum culture mediums of the prior art, the serum-free stem cell culture medium has the advantages that quality difference among serum batches can be avoided, and repeatability of cell culture and experiment results is improved; exogenous pollution caused by serum and cytotoxic effect of serum components are avoided. The serum-free stem cell culture medium has relatively clear own ingredients, uniform quality, low protein content and is conducive to improving stability of cell product production and easy to purify. In addition, after stem cell proliferation and property degrees are maintained through several specific matching modes, high-density cell culture can be maintained for a long time by prolonging GI period of cells or forcing the cells to be in G0 period, and stem cell characteristics can be retained better.

Description

technical field [0001] The invention belongs to the technical field of cell culture medium, and in particular relates to a serum-free stem cell culture medium and an application thereof. Background technique [0002] Stem cells cannot survive in simple basal medium, and certain trace nutrients and growth factors must be provided in cell culture to enable cells to grow and maintain a growth state. Therefore, serum, such as horse serum or fetal bovine serum, is often added to the basal medium used; because serum is an extremely complex mixture of many biomolecules of different sizes, it can provide growth factors and hormones needed by cells in vitro , binding proteins, and provide protection; and the serum itself is also rich in mitotic factors, which is also beneficial to cell lines and primary culture. However, there are certain disadvantages in the serum medium, which cannot avoid the heterologous body in the animal serum. If the cells cultivated in the animal serum mediu...

AI Technical Summary

Problems solved by technology

However, there are certain disadvantages in the serum medium, which cannot avoid the heterologous body in the animal serum. If the cells cultivated in the animal serum medium are used, there will be rejection and conflict of the human heterologous body.

[0003] Especially in the process of stem cell culture, a large number of complex proteins in serum bring difficulties to the standardization of cell culture, among which complex proteins and multiple cytokines will cause stem cells that are easy to differentiate themselves to produce a variety of completely different Cell phenotype, resulting in a variety of completely different cell differentiation trends, also brings great difficulties to the separation and purification of cell culture expression products and the reproducibility of results

Images