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Preparation method suitable for industrialized continuous production of enzyme modified soybean phospholipids

A technology for modifying and concentrating phospholipids, applied in the field of bioengineering, to achieve the effects of strong emulsifying performance, improved production efficiency and fewer operators

Active Publication Date: 2015-11-25
李桂华 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] For phospholipase A2, phospholipase A1 and 1,3-lipase aqueous solution, without organic solvents and additives, the enzymolysis of industrialized soybean lecithin, and the continuous concentration and drying of soybean lecithin after enzymolysis by a fixed scraper vacuum film evaporator Methods for dehydration, inactivation of enzymes, cooling and prevention of enzymatic phospholipid oxidation have not yet been reported in the literature and implemented by patented technologies

Method used

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  • Preparation method suitable for industrialized continuous production of enzyme modified soybean phospholipids

Examples

Experimental program
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Effect test

Embodiment 1

[0029] 1000kg of concentrated soybean lecithin with an acid value of 19.5KOHmg / g is pumped into the lecithin stirring tank by a delivery pump, heated to make the temperature of soybean lecithin in the lecithin tank reach 70°C, and slowly add 5.0kg of soybean lecithin under stirring condition of 350rpm / min Phospholipase A1 (enzyme activity 1000IU / 100g) and 50 kg of distilled water were used for enzymatic hydrolysis for 28 hours to make the acid value of soybean lecithin 64.0KOHmg / g. Then the screw pump is pumped into a fixed scraper film evaporator with a temperature of 160°C, an absolute pressure of 3.0KPa, and a rotation speed of 300rpm for evaporating and dehydrating for 3 minutes, so that the moisture content of soybean phospholipids is below 0.67%, and then pumped into a phospholipid cooler by a screw pump for cooling When it reaches 58°C, put it into a vacuum stirring tank to deoxygenate the modified phospholipids, then weigh and pack.

Embodiment 2

[0031] 800kg of concentrated peanut phospholipids with an acid value of 21.6KOHmg / g is pumped into the phospholipid stirring tank by the delivery pump, heated to make the temperature of the peanut phospholipid in the phospholipid stirring tank reach 55°C, and slowly add 6kg of peanut phospholipids under stirring conditions of 450rpm / min Phospholipase A1 (enzyme activity 6000IU / 100g) and 80 kilograms of distilled water were used for enzymatic hydrolysis for 16 hours to make the acid value of peanut phospholipid 55.0KOHmg / g. Then the screw pump is pumped into the fixed scraper film evaporator with a temperature of 150°C, an absolute pressure of 5.0KPa, and a rotation speed of 400rpm for evaporating and dehydrating for 3 minutes, so that the water content of the concentrated peanut phospholipids is below 0.81%, and then pumped into the phospholipid cooler by the screw pump Cool down to 62°C and put into a vacuum stirring tank to deoxygenate the modified phospholipids, then weigh a...

Embodiment 3

[0033] 2000kg of concentrated soybean lecithin with an acid value of 22.5KOHmg / g is pumped into the phospholipid stirring tank by the delivery pump, heated to make the temperature of the soybean lecithin in the phospholipid stirring tank reach 60°C, and slowly add 55kg The phospholipase A1 and phospholipase A2 mixed solution (enzyme activity 2000IU / 100g) and 160 kilograms of distilled water, enzyme hydrolysis 18h makes the acid value of soybean lecithin 58.2KOHmg / g. Then the screw pump is pumped into the fixed scraper film evaporator with a temperature of 130°C, an absolute pressure of 2.0KPa, and a rotation speed of 300rpm for evaporating and dehydrating for 3.5 minutes, so that the moisture content of soybean phospholipids is below 0.42%, and then pumped into the phospholipid cooler by a screw pump After cooling down to 60°C, put it into a vacuum stirring tank to deoxygenate the modified phospholipids, then weigh and pack.

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Abstract

The invention discloses a preparation method suitable for industrialized continuous production of enzyme modified concentrated phospholipids. The preparation method comprises the following steps: a, adding phospholipase A1, phospholipase A2, and a 1, 3-lipase aqueous solution into concentrated phospholipids for enzymatic modification reaction; b, pumping the concentrated soybean phospholipids after enzymatic modification into a fixed scraper film evaporator for dehydration by continuous evaporation and enzyme inactivation through a screw pump, so as to enable the moisture to be decreased to 1% or below; c, concentrating, dewatering, cooling the inactivated enzyme modified phospholipids with a cooler, and introducing the cooled enzyme modified phospholipids into a vacuum stirring tank for removing oxygen in the modified phospholipids, so as to obtain the enzyme modified soybean phospholipids with good storage stability. The preparation method is suitable for continuous industrial production, the processing technology is easy and convenient, the production efficiency is remarkably improved, and the produced enzyme modified soybean phospholipids are good in dispersity, hydrophilia and heat resistance in water, quite good in emulsion stability in a low pH value range and hyper-salt concentration, and widely applicable to the industries, such as, food, medicine, daily-use chemicals, pesticides, paint coatings, leather and textile, petrochemical industry and fodder.

Description

technical field [0001] The invention relates to the application of biological enzymes in oils and lipids, and belongs to the technical field of biological engineering, in particular to a preparation method for industrialized continuous production of enzyme-modified soybean phospholipids. Background technique [0002] Concentrated soybean lecithin usually contains 60% phospholipids, 40% oil, and is composed of phosphatidylcholine (PC), phosphatidylcholine (PE), phosphatidylinositol (PI), phosphatidic acid, glycolipids and glycerides and other components , the HLB value is low (3.0~4.2), and the solubility, dispersion, and emulsification properties in the water phase are poor, which limits the application of concentrated soybean lecithin in industries such as hydrophilic systems. Therefore, it is necessary to modify the concentrated soybean lecithin to improve hydrophilicity. water performance. [0003] At present, the enzymatic modification of concentrated phospholipids uses...

Claims

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Application Information

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IPC IPC(8): C12P9/00C12P7/64
Inventor 李桂华李晓洁
Owner 李桂华
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