Pichia pastoris for producing methanol protein and lipase at same time and application thereof

A technology of methanol protein and Pichia pastoris, applied in the field of bioengineering, can solve the problems of outdated fermentation process, single product, high cost, etc., and achieve the effects of simplifying the production process, improving production efficiency and reducing raw material consumption

Active Publication Date: 2015-12-09
义马煤业集团煤生化高科技工程有限公司
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Problems solved by technology

[0005] In view of the above situation, the purpose of the present invention is to provide a strain of Pichia pastoris that simultaneously produces methanol protein and lipase and its application, which can effectively solve the problems of single product, outdated fermentation process, high cost and low benefit in the existing methanol protein production. the puzzle

Method used

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Embodiment

[0025] (1) Cultivation of first-class seeds: YPD medium is used as the first-class seed medium, which is divided into 2000mL triangular flasks, with a liquid volume of 500mL per bottle, wrapped with 8 layers of gauze, sterilized at 121°C for 30 minutes, and cooled for later use. Take 3-5 strains of strain zfwx-208 preserved on the slant of the test tube (also known as the slant of the test tube), scrape it off with an inoculation loop and put it into a triangular flask with 500mL of first-level seed medium, inoculate two bottles on the slant of each test tube, and inoculate Afterwards, shaking and culturing in a shaker at 30°C for 22-26 hours, with a shaker speed of 200-300rpm, to obtain first-grade shake flask seeds;

[0026] (2) Cultivation of secondary seeds: Secondary seed medium adopts inorganic salt medium, sterilized at 121°C for 30 minutes and cooled down, then add 4mL of sterilized by 0.22μm microporous membrane filter to each liter of inorganic salt medium Trace elem...

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Abstract

The invention relates to a pichia pastoris for producing methanol protein and lipase at the same time and application thereof. The problems that during existing production of methanol protein, products are single, the fermentation technology is old, cost is high, and benefits are low are effectively solved. The pichia pastoris for producing methanol protein and lipase at the same time is named as pichia pastoris zfwx-208 strain and preserved in the China center for type culture collection, the preservation number is CCTCC NO.M2013518, the preservation date is October 30th, 2013, and the preservation address is the China center for type culture collection in Wuhan university of Wuhan city in China. Because of combined fermenting production of lipase and methanol protein, a device is multipurpose, production cost is reduced, production benefits are improved, the production technology is simplified, raw material consumption is reduced, energy in use is saved, and environmental pollution is relieved.

Description

technical field [0001] The invention relates to the field of bioengineering, in particular to a strain of Pichia pastoris capable of simultaneously producing methanol protein and lipase and its application. Background technique [0002] Lipase is a class of ester bond hydrolase that mainly hydrolyzes triglycerides formed from glycerol and water-insoluble long-chain fatty acids. It is widely used in food processing, new biomaterials, biomedicine, chiral drug resolution and other fields. The application of lipase in baked food can strengthen the gluten, improve the texture of dough and the quality of bread. The hydrolysis of milk fat by lipase can improve the quality of dairy products, and can be used to enhance the flavor of cheese and milk powder, cheese ripening, esterification modification of cream and ice cream, etc. Lipase is mainly used in the pharmaceutical industry to aid digestion and reduce blood fat, and can also be used for Clinical diagnosis of lipemia, pancreat...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12N9/20C12R1/84
Inventor 苟万晓杨刚任晓辉胡元森卫红伟范延超白冠章谢军李志红郝景梅马敏徐倩段亚培陈艳冰尹聪乐
Owner 义马煤业集团煤生化高科技工程有限公司
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