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Recombinant swine pox virus vector vaccine expressing Salmonella typhimurium ompl protein

A technology of Salmonella typhimurium and recombinant swinepox virus, applied in the direction of virus/phage, recombinant DNA technology, resistance to vector-borne diseases, etc., can solve the problem of no vaccine or drug for preventing Salmonella typhimurium, and achieve good protection effect of action

Active Publication Date: 2018-06-08
NANJING AGRICULTURAL UNIVERSITY +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the use of SPV vectors to construct recombinant pox virus expressing the outer membrane protein L of Salmonella typhimurium, and there is no vaccine or drug for preventing Salmonella typhimurium using SPV vectors to express the outer membrane protein L of Salmonella typhimurium. to report

Method used

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  • Recombinant swine pox virus vector vaccine expressing Salmonella typhimurium ompl protein
  • Recombinant swine pox virus vector vaccine expressing Salmonella typhimurium ompl protein
  • Recombinant swine pox virus vector vaccine expressing Salmonella typhimurium ompl protein

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Experimental program
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Effect test

Embodiment 1

[0036] 1 Cloning and identification of ompL gene

[0037] 1.1 PCR amplification of ompL gene

[0038] A pair of specific primers for the ompL gene were designed according to the gene sequence of Salmonella typhimurium (GenBank: AE006468.1), and the 5' ends of the primers contained restriction endonucleases SalI and BamHI respectively. Primers were synthesized by Nanjing GenScript Biotechnology Co., Ltd. The sequence is as follows:

[0039] P1: 5'-CAGGTCGACGGCGCTTATGTAGAAAAACC-3' (SEQ ID NO.2)

[0040] P2: 5'-CTAGGATCCTCAGAAGAAATACTTCGCCC-3' (SEQ ID NO. 3).

[0041] Take the Salmonella typhimurium JSSQ14 strain isolated from the intestinal tract of sick piglets in a large-scale pig farm in Suqian, Jiangsu Province in 2014 (the purification method of this strain belongs to conventional technology, and the public can obtain it again through ordinary methods. This strain is preserved by our laboratory , willing to provide to the public for research) genome as a template, the omp...

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Abstract

The invention belongs to the field of biological products and provides a coding gene of salmonella typhimurium outer membrane protein L, a transfer vector pUSG11 / P28-OmpL with the coding gene, a recombinant swinepox virus rSPV-OmpL and application and a preparation method of the recombinant swinepox virus rSPV-OmpL. The sequence of the coding gene is shown as SEQ ID NO.1, and the recombinant swinepox virus is obtained by insertion of the salmonella typhimurium outer membrane protein L into swinepox virus VR-363. A recombinant swinepox virus vaccine rSPV-OmpL is capable of greatly expressing target protein OmpL in immune animal bodies to induce in-vivo generation of high-potency antibodies aiming at OmpL in the immune animal bodies, the immune animals can be well protected, and the immune protective rate is up to 80% as for mice. In addition, the vaccine integrates advantages of excellent immune effects of attenuated vaccines and high safety of inactivated vaccines, thereby being an ideal novel vaccine.

Description

technical field [0001] The invention belongs to the field of biological products, and relates to a recombinant pox virus vector vaccine expressing Salmonella typhimurium OmpL protein, in particular to a gene encoding outer membrane protein L of Salmonella typhimurium, and a transfer vector pUSG11 / P28 including the gene - OmpL, recombinant swine pox virus rSPV-OmpL and its application and preparation method. Background technique [0002] Salmonella typhimurium (Salmonella typhimurium) is an important zoonotic pathogen, and its infection incidence ranks first in Salmonella infection. Salmonella typhimurium is widely distributed in nature, mainly transmitted through water and food, and can cause various diseases in humans and animals. Salmonella typhimurium infects piglets and can cause swineparatyphoid in piglets. It mainly causes septicemia, enteritis, pneumonia and hepatitis in pigs, and can also cause abortion in pregnant sows. With the intensive development of pig farmi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/31C12N15/63C12N15/863C12N7/01A61K48/00A61K39/112A61P31/04
CPCY02A50/30
Inventor 范红结周红蔺辉星方一臻
Owner NANJING AGRICULTURAL UNIVERSITY