Method for extracting buckwheat protein from buckwheat

A buckwheat and protein technology, applied in the field of buckwheat protein extraction, can solve the problems of high production cost, low protein purity, large consumption, etc., and achieve the effects of simplified operation, simple operation, and avoidance of waste and pollution

Inactive Publication Date: 2015-12-16
内蒙古清谷新禾有机食品集团有限责任公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The excellent selectivity and easy-to-separation characteristics of ionic liquids can quickly and easily separate high-purity proteins, thereby solving the problems of high consumption of extraction water and high production costs caused by the

Method used

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Examples

Experimental program
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Example Embodiment

[0033] Example 1

[0034] The method for extracting buckwheat protein from buckwheat according to the present invention includes the following steps:

[0035] Take buckwheat flour, add 1 times the weight of buckwheat flour to a 0% ethanol solution (0% ethanol solution includes 0.01% disodium hydrogen phosphate, sodium dihydrogen phosphate and sodium bicarbonate), stir and extract at 0°C for 24 Hours, after the extraction, the buckwheat protein extract is separated;

[0036] Add polyethylene glycol 800 to the buckwheat protein extract to precipitate at 0°C while stirring, and collect the precipitate after the precipitation is complete;

[0037] Add water to the precipitate to make the protein concentration 1.00% to obtain a protein solution. Add ionic liquid (the cation is an alkyl quaternary phosphonium cation, and the anion is bis(trifluoroformyl)amide) to make the ions The liquid concentration is 0.01g / L, stirred at 0°C for 12 hours, and allowed to stand for 12 hours to fully divid...

Example Embodiment

[0040] Example 2

[0041] The method for extracting buckwheat protein from buckwheat of the present invention includes the following steps:

[0042] Take buckwheat flour, add 20 times the weight of buckwheat flour to a 15% ethanol solution (15% ethanol solution includes 15% ammonium chloride), stir and extract at 30°C for 16 hours. After the extraction is completed, buckwheat protein is isolated Extract

[0043] Add ZTC1+1 series clarifying agent to precipitate the buckwheat protein extract at 30°C while stirring, and collect the precipitate after the precipitation is complete;

[0044] Water was added to the precipitate to make the protein concentration 20.00% to obtain a protein solution, and ionic liquid (the cation is alkylimidazole ion, the anion is tetrafluoroborate) was added to the protein solution to make the ionic liquid concentration 40.00g / L. Stir at 20°C for 8 hours, and let stand for 8 hours to fully divide the liquid into two phases and separate the ionic liquid phase...

Example Embodiment

[0047] Example 3

[0048] The method for extracting buckwheat protein from buckwheat of the present invention includes the following steps:

[0049] Take buckwheat flour, add 30 times the weight of buckwheat flour to a 25% ethanol solution (25% ethanol solution includes 25% potassium sulfate and potassium nitrate), stir and extract at 50°C for 8 hours. After the extraction is complete, separate it. Buckwheat protein extract;

[0050] Add the ZTC101 series clarifying agent to the buckwheat protein extract at 50°C while stirring, and collect the precipitate after the precipitation is complete;

[0051] Water was added to the precipitate to make the protein concentration 30.00% to obtain a protein solution, and ionic liquid (the cation is alkyl pyridinium ion, the anion is hexafluorophosphoric acid) was added to the protein solution to make the ionic liquid concentration 60.00 g / L. Stir at 40°C for 4 hours, and let it stand for 4 hours to fully divide the liquid into two phases and sep...

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PUM

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Abstract

The invention discloses a method for extracting buckwheat protein from buckwheat. The method comprises the following steps: adding taken buckwheat powder to an extract solution, stirring and extracting the buckwheat powder, ending extraction and separating the buckwheat powder to obtain a buckwheat protein extract solution; adding a precipitant while stirring the buckwheat protein extract liquid, precipitating completely and collecting a sediment; adding water to the sediment to obtain a protein solution; adding an ionic liquid to the protein solution, stirring, standing, dividing the liquid into two phases, and separating out an ionic liquid phase; adding purified water to the ionic liquid phase, stirring, standing, dividing the liquid into two phases, and separating out a water phase; and drying the water phase, and removing moisture, so as to prepare the buckwheat protein. The method for extracting the buckwheat protein from the buckwheat disclosed by the invention has the advantages of being high in extraction rate, high in extraction speed and simple to operate; pollution to the environment and hidden dangers in safety production due to use of strong acid and alkali are avoided; and the precipitant is a purely natural or food-grade material, and is safe, nontoxic, environmentally friendly, and good in enrichment effect on protein.

Description

technical field [0001] The invention belongs to the technical field of plant extraction, and in particular relates to a method for extracting buckwheat protein from buckwheat. Background technique [0002] Proteins are biomacromolecules with a certain spatial structure. Strong interactions such as hydrogen bonds and ionic bonds between molecules and molecules and the hydrophobic characteristics of molecules make proteins difficult to dissolve in general organic solvents. Normally, proteins can be dissolved in strong acid, strong alkali, and high-concentration salt solutions, but these solvents can easily cause protein denaturation and degradation, thereby affecting the performance of proteins. Therefore, finding an ideal solvent that can dissolve and stabilize proteins is one of the problems that need to be solved urgently in the current protein chemistry research. [0003] At present, protein extraction methods include alkali extraction and acid precipitation, acid extract...

Claims

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Application Information

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IPC IPC(8): A23J1/12
Inventor 刘宏王焕芸白玉琴肖志彬雷露静郭金龙辛振涛董建国崔志丰
Owner 内蒙古清谷新禾有机食品集团有限责任公司
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