Method for extracting buckwheat protein from buckwheat

Abstract

The invention discloses a method for extracting buckwheat protein from buckwheat. The method comprises the following steps: adding taken buckwheat powder to an extract solution, stirring and extracting the buckwheat powder, ending extraction and separating the buckwheat powder to obtain a buckwheat protein extract solution; adding a precipitant while stirring the buckwheat protein extract liquid, precipitating completely and collecting a sediment; adding water to the sediment to obtain a protein solution; adding an ionic liquid to the protein solution, stirring, standing, dividing the liquid into two phases, and separating out an ionic liquid phase; adding purified water to the ionic liquid phase, stirring, standing, dividing the liquid into two phases, and separating out a water phase; and drying the water phase, and removing moisture, so as to prepare the buckwheat protein. The method for extracting the buckwheat protein from the buckwheat disclosed by the invention has the advantages of being high in extraction rate, high in extraction speed and simple to operate; pollution to the environment and hidden dangers in safety production due to use of strong acid and alkali are avoided; and the precipitant is a purely natural or food-grade material, and is safe, nontoxic, environmentally friendly, and good in enrichment effect on protein.

Description

technical field [0001] The invention belongs to the technical field of plant extraction, and in particular relates to a method for extracting buckwheat protein from buckwheat. Background technique [0002] Proteins are biomacromolecules with a certain spatial structure. Strong interactions such as hydrogen bonds and ionic bonds between molecules and molecules and the hydrophobic characteristics of molecules make proteins difficult to dissolve in general organic solvents. Normally, proteins can be dissolved in strong acid, strong alkali, and high-concentration salt solutions, but these solvents can easily cause protein denaturation and degradation, thereby affecting the performance of proteins. Therefore, finding an ideal solvent that can dissolve and stabilize proteins is one of the problems that need to be solved urgently in the current protein chemistry research. [0003] At present, protein extraction methods include alkali extraction and acid precipitation, acid extract...

AI Technical Summary

Problems solved by technology

[0008] The excellent selectivity and easy-to-separation characteristics of ionic liquids can quickly and easily separate high-purity proteins, thereby solving the problems of high consumption of extraction water and high production costs caused by the extensive use of strong acids and strong alkalis in existing extraction technologies. , A large amount of waste water produced by production will seriously pollute the environment, and the extracted protein has low purity and difficult purification.