A Strain of Halomonas tf-1 and Its Application

A technology of TF-1, Halomonas, applied in the field of microbial biotechnology and environmental biology

Active Publication Date: 2018-09-04
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many strains resistant to high temperature and high salt in the environment, but there are few strains resistant to high temperature and salt and capable of degrading crude oil emulsification in microbial oil recovery, and there are reports on strains applied to high temperature and high salt reservoirs Therefore, in view of the above situation, it is necessary to screen out halophilic bacteria suitable for high-temperature and high-salt reservoir environment and apply them to microbial oil recovery technology

Method used

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  • A Strain of Halomonas tf-1 and Its Application
  • A Strain of Halomonas tf-1 and Its Application
  • A Strain of Halomonas tf-1 and Its Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Morphological characteristics and physiological and biochemical characteristics of Halomonas TF-1CGMCC No.10619 provided by the present invention.

[0035] Refer to the experimental method of "Bergey's Mannual of Systematic Bacteriology" to detect its Gram staining, cell size and morphology, presence or absence of flagella and spores, growth temperature, as well as catalase, starch hydrolysis, gelatin liquefaction, galactose, xylose , lactose and other biochemical characteristics.

[0036] The research results show that if figure 1 As shown in the figure, the strain is Gram-positive, with short rod-shaped cells, without flagella, motile and aerobic, with a cell size of 4-8 μm × 0.2-0.5 μm, and a growth temperature of 30-60 °C. The growth temperature is 37°C, the growth pH range is 5~8, the optimal growth pH range is 7~7.5, and the NaCl tolerance is 0~26%, such as figure 2 .

Embodiment 2

[0038] The culture fermentation and fermentation broth performance evaluation of Halomonas TF-1CGMCC No.10619 provided by the present invention

[0039] Fermentation medium: glucose 0.3mg / L, peptone 0.3mg / L, yeast powder 0.3mg / L, K 2 HPO 4 0.27mg / L, sodium chloride 6mg / L, pH 7.0.

[0040] Fermentation conditions: inoculum size 2%, inoculation age 12h, initial pH 7.0, temperature 37°C, stirring speed 180rpm, pH=7, ventilation rate: 1L / min, evaluation after culturing for 24h.

[0041] Evaluation and results of performance parameters of fermentation broth:

[0042] (1) Evaluation of growth ability with crude oil as the sole carbon source

[0043] Halomonas TF-1CGMCC No. 10619 was inoculated in a hydrocarbon medium with crude oil as the sole carbon source, shaken at 37 ° C and 180 rpm, and sampled at different times for bacterial density testing, such as image 3 The experimental results show that the strain grows with crude oil as the only carbon source, and the bacterial con...

Embodiment 3

[0057] The Halomonas TF-1CGMCC No.10619 fermentation broth provided by the invention is applied in single well huff and puff of oil well C in Shengli Oilfield.

[0058] Oil well overview: formation temperature 60℃, formation water salinity 2.4×10 4 mg / L, the effective thickness of the oil layer is 6.7m, the porosity is 0.30, and the permeability is 551×10 -3 μm 2 .

[0059] Implementation steps: 100m of Halomonas TF-1 fermentation broth 3 Use a pump truck to pass through the oil casing annulus of oil well C to 8m 3 The injection rate of / h was injected into the formation, and the production was resumed after the well was shut in for 10 days.

[0060] Test results: After the test was completed, both the liquid and oil production of the well increased, with a cumulative oil increase of 280t in 6 months.

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Abstract

The invention discloses halomonas TF-1 and application thereof. The strain of the halomonas TF-1 is obtained from an oil-water well sample of the Shengli Oil Field under the conditions of 37 DEG C temperature and ordinary pressure by using a high-salt culture medium containing 6.0% of NaCl through enrichment culture screening, temperature-resistant salt-resistant performance investigation, repeated screening and passage, is named as halomonas sp.TF-1, and the preservation number of the halomonas TF-1 is CGMCC No.10619. The nutrient culture medium of the halomonas TF-1 is prepared from 0.3 mg / L of glucose, 0.3 mg / L of peptone, 0.3 mg / L of yeast powder, 0.27 mg / L of K2HPO4 and 6 mg / L sodium chloride, and a pH value is 7.0 -7.5. The halomonas TF-1 grows with crude oil as a carbon source, the bacterial concentration is greater than 5*108 per milliliter. The emulsification viscosity reduction rate of fermentation liquid is higher than 90%. The recovery ratio improved in a physical simulation experiment is higher than 15%. The average oil increase amount of microbial single well stimulation is more than 250 tons. An averagely prolonged hot washing period of microbial paraffin control is greater than 120d. Therefore, the halomonas TF-1 can be widely applied to a field test of microbial single well treatment.

Description

technical field [0001] The invention belongs to the field of microbial biotechnology and environmental biotechnology, and particularly relates to a Halomonas TF-1 strain and its application in microbial oil recovery. Background technique [0002] Microbial oil recovery relies on microbial metabolism to generate acid, carbon dioxide or biogas to expand the pores of oil-bearing layers and increase the fluidity of crude oil; or to decompose heavy hydrocarbons into more mobile short-chain compounds, as well as to produce biosurfactants, biopolymers to enhance oil recovery. However, the reservoir environment is often characterized by high temperature and high salinity. To carry out microbial oil recovery in these reservoirs, it is necessary to have high-efficiency strains that are resistant to temperature and salt. There are many strains that are resistant to high temperature and high salt in the environment, but there are few strains that are resistant to high temperature and s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C09K8/582C12R1/01
Inventor 谭晓明冯云宋欣王静林军章徐登霆巴燕徐闯段传慧刘涛
Owner CHINA PETROLEUM & CHEM CORP
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