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Application of Candida tropicalis in producing dodecanedioic acid from unsaturated oil

A technology of Candida tropicalis and dodecanediacid, applied in the field of bioengineering, can solve problems such as increased production cost of dibasic acid, and achieve the effects of simple and easy process, low cost of raw materials and reduction of production cost

Active Publication Date: 2018-10-30
QILU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The raw materials for the production of long-chain dibasic acids, especially dodecane dibasic acids, by the above method are all alkanes, while the decline in crude oil reserves and the shortage of petrochemical raw material resources required for the production of long-chain dibasic acids have led to a decrease in the production cost of dibasic acids. increase, so it is urgent to find new sustainable alternatives, but there is no report on the use of microorganisms to catalyze long-chain unsaturated oils to produce dodecanedioic acid

Method used

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  • Application of Candida tropicalis in producing dodecanedioic acid from unsaturated oil
  • Application of Candida tropicalis in producing dodecanedioic acid from unsaturated oil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1, Catalyzing the Screening of Unsaturated Fatty Acid Unsaturated Bond Breaking Bacteria in Catalytic Oil Components

[0035] Candida sake CICC 33015, Candida lipolytica CICC 1457, Candida tropicalis CICC 1798 purchased from China Industrial Microorganism Culture Collection Management Center and dibasic acid high-yielding yeast Candida preserved in the Institute of Enzyme Engineering, Qilu University of Technology After activation, tropicalis IEE001 was inoculated into the screening medium, in which the medium components were yeast extract powder 10g / L, peptone 20g / L, glucose 20g / L, peanut oil 50ml / L, tap water configuration, natural pH, 115 °C for 30 minutes. After the fermentation, the fermented liquid was adjusted to pH 12, then heated to 90°C, and treated for 30 minutes. Centrifuge at 7000 rpm for 5 minutes while hot to remove bacteria. Adjust the pH value of the supernatant in the above steps to about 2.95, heat again to 90°C and keep for 30min; slowly co...

Embodiment 2

[0037] Embodiment 2, fermenting corn oil in 500mL shake flask produces dodecanedioic acid

[0038] The application of Candida tropicalis in the production of dodecanedibasic acid by using unsaturated oil, the steps are as follows:

[0039](1) Inoculate 5 mL of Candida tropicalis seed liquid into 100 mL of fermentation medium, and add glucose solution every 12 hours at a temperature of 30°C and a rotational speed of 210 r / min, so that the glucose concentration is 6.2%, and adjust once at the same time pH control the pH of the fermentation broth to 6.0, and ferment for 24 hours; then, add corn oil every 12 hours to make the oil mass concentration 10%, control the pH of the fermentation broth to 7.5, and ferment for 120 hours to obtain the fermentation broth;

[0040] Fermentation medium in described step (1), component is as follows:

[0041] Glucose 50g / L, (NH 4 ) 2 SO 4 1g / L, corn syrup 1g / L, yeast extract 2g / L, VB 1 0.1g / L, NaCl 2g / L, KH 2 PO 4 6g / L, Na 2 HPO 4 12...

Embodiment 3

[0048] Embodiment 3, fermenting corn oil in 5L fermentation tank produces dodecane dibasic acid

[0049] The application of Candida tropicalis in the production of dodecanedibasic acid by using unsaturated oil, the steps are as follows:

[0050] (1) Inoculate 300mL of Candida tropicalis seed liquid into 3L fermentation medium, and add glucose solution under the conditions of temperature 30°C, ventilation ratio 1:0.7vvm, and tank pressure 0.08Mpa, so that the glucose concentration is 6.2% , control the pH of the fermentation broth to 6.0, and ferment for 24 hours; then, add unsaturated oil to make the oil mass concentration 10%, control the pH of the fermentation broth to 7.5, and ferment for 120 hours to obtain the fermentation broth;

[0051] Fermentation medium in described step (1), component is as follows:

[0052] Glucose 40g / L, (NH 4 ) 2 SO 4 1g / L, corn syrup 1g / L, yeast extract 2g / L, VB 1 0.1g / L, NaCl 2g / L, KH 2 PO 4 4g / L, Na 2 HPO 4 12H 2 O 10.08g / L, urea ...

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Abstract

The invention relates to application of candida tropicalis to preparation of dodecanedioic acid by utilizing unsaturated grease. The candida tropicalis is derived from the China's industrial microbial strain preservation management center, and the strain number is CICC 1798. The condition that when the candida tropicalis CICC 1798 is fermented, unsaturated double bonds in long-chain unsaturated fatty acid component (octadeca-carbon unsaturated fatty acid) in grease can be subjected to fragmentation and addition to obtain dodecanedioic acid is discovered for the first time, so that limit of petrochemical products as the raw materials for long-chain binary acid preparation is broken through, and a new path for synthesizing dodecanedioic acid is provided.

Description

technical field [0001] The invention relates to the application of candida tropicalis in producing dodecanedibasic acid by using unsaturated oil, and belongs to the technical field of bioengineering. Background technique [0002] Long-chain dibasic acids refer to aliphatic dicarboxylic acids with more than 7 carbon atoms in the carbon chain. They are a class of fine chemical products with important and extensive industrial uses. Important raw materials for plastics, high-grade nylon hot-melt adhesives, high-temperature dielectrics, high-grade paints and coatings, high-grade lubricating oils, cold-resistant plasticizers, resins, medicines and pesticides, etc. [0003] In the early days, long-chain dibasic acids were mainly produced by chemical methods. However, due to the shortcomings of high pollution and high energy consumption in the preparation process, and high requirements for production equipment, they mainly rely on microbial fermentation to obtain long-chain dibasic ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/64C12R1/74
Inventor 王瑞明杜鹏汪俊卿王腾飞杨晓慧马春玲范翰程成石莹
Owner QILU UNIV OF TECH
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