Application of candida tropicalis to preparation of dodecanedioic acid by utilizing unsaturated grease
A technology of Candida tropicalis and dodecanediacid, applied in the field of bioengineering, can solve problems such as increased production cost of dibasic acid, and achieve the effects of simple and easy process, reduced production cost, and low cost of raw materials
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Embodiment 1
[0034] Example 1, Catalyzing the Screening of Unsaturated Fatty Acid Unsaturated Bond Breaking Bacteria in Catalytic Oil Components
[0035] After activating Candida sake CICC33015, Candida lipolytica CICC1457, Candida tropicalis CICC1798 purchased from China Industrial Microbiology Culture Collection Management Center and dibasic acid high-yielding yeast CandidatropicalisIEE001 preserved in the Institute of Enzyme Engineering, Qilu University of Technology , respectively inoculated into the screening medium, wherein the medium components are yeast extract powder 10g / L, peptone 20g / L, glucose 20g / L, peanut oil 50ml / L, tap water configuration, natural pH, sterilized at 115°C for 30 minutes . After the fermentation, the fermented liquid was adjusted to pH 12, then heated to 90°C, and treated for 30 minutes. Centrifuge at 7000 rpm for 5 minutes while hot to remove bacteria. Adjust the pH value of the supernatant in the above steps to about 2.95, heat again to 90°C and keep for ...
Embodiment 2
[0037] Embodiment 2, fermenting corn oil in 500mL shake flask produces dodecanedioic acid
[0038] The application of Candida tropicalis in the production of dodecanedibasic acid by using unsaturated oil, the steps are as follows:
[0039](1) Inoculate 5 mL of Candida tropicalis seed liquid into 100 mL of fermentation medium, and add glucose solution every 12 hours at a temperature of 30°C and a rotational speed of 210 r / min, so that the glucose concentration is 6.2%, and adjust once at the same time pH control the pH of the fermentation broth to 6.0, and ferment for 24 hours; then, add corn oil every 12 hours to make the oil mass concentration 10%, control the pH of the fermentation broth to 7.5, and ferment for 120 hours to obtain the fermentation broth;
[0040] Fermentation medium in described step (1), component is as follows:
[0041] Glucose 50g / L, (NH 4 ) 2 SO 4 1g / L, corn syrup 1g / L, yeast extract 2g / L, VB 1 0.1g / L, NaCl2g / L, KH 2 PO 4 6g / L, Na 2 HPO 4 12H 2...
Embodiment 3
[0048] Embodiment 3, fermenting corn oil in 5L fermentation tank produces dodecane dibasic acid
[0049] The application of Candida tropicalis in the production of dodecanedibasic acid by using unsaturated oil, the steps are as follows:
[0050] (1) Inoculate 300mL of Candida tropicalis seed liquid into 3L fermentation medium, and add glucose solution under the conditions of temperature 30°C, ventilation ratio 1:0.7vvm, and tank pressure 0.08Mpa, so that the glucose concentration is 6.2% , control the pH of the fermentation broth to 6.0, and ferment for 24 hours; then, add unsaturated oil to make the oil mass concentration 10%, control the pH of the fermentation broth to 7.5, and ferment for 120 hours to obtain the fermentation broth;
[0051] Fermentation medium in described step (1), component is as follows:
[0052] Glucose 40g / L, (NH 4 ) 2 SO 4 1g / L, corn syrup 1g / L, yeast extract 2g / L, VB 1 0.1g / L, NaCl2g / L, KH 2 PO 4 4g / L, Na 2 HPO 4 12H 2 O10.08g / L, urea 2g / L,...
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