Compound microorganism feed additive and preparation method thereof
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A technology of compound microorganisms and feed additives, which is applied in animal feed, animal feed, application and other directions to achieve the effect of reducing mortality, reducing environmental pollution and eliminating adverse effects
Inactive Publication Date: 2015-12-23
WUHAN XINXINGDI BIOTECH DEV
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[0005] In order to solve the above-mentioned shortcomings of the existing medicinal feed additives and the shortcomings of a single strain of microbial feed additives, the present invention provides a composite microbial feed additive that uses solid fermentation technology to produce the composite microbial feed additive
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[0051] ① Preparation of primary strains
[0052] Aspergillus niger test tube plate (PDA solid medium) was streaked or diluted and spread on the plate, and cultured at 30°C for 2-3 days to obtain a single colony. Pick a single colony of spores and inoculate them into a bran test tube, and incubate at a constant temperature of 30°C for 2-3 days until Aspergillus niger grows to the bottom of the test tube and turns black.
[0053] ②Preparation of secondary species
[0054] Use an inoculation shovel to inoculate the test tube bacteria into a triangular flask filled with bran medium. The sample volume of the triangular flask is 20-25g. In order to ensure sufficient oxygen supply, two layers of newspaper are used to seal. Cultivate at a constant temperature of 30°C for 24 hours, turn over the bottle, break up the agglomerates and continue to cultivate, and stop culturing after 48-60 hours.
[0055] ③Extended cultivation of three-level strains in canned bottles
[0056] Add twice ...
Embodiment 1
[0091] A compound microbial feed additive is composed of the following raw materials in mass fractions: 60 parts of Aspergillus niger agent, 20 parts of Bacillus subtilis, 15 parts of Enterococcus faecalis and 5 parts of oligosaccharide.
[0092] The live cell count of each strain in the compound microbial feed additive is: the number of spores of Aspergillus niger 3*10 8 cfu / g, Bacillus subtilis 10*10 9 cfu / g, Enterococcus faecalis 10*10 9 cfu / g, total bacterial count 5*10 9 cfu / g.
Embodiment 2
[0094] A compound microbial feed additive is composed of the following raw materials in mass fractions: 50 parts of Aspergillus niger fungicide, 20 parts of Bacillus subtilis, 20 parts of Enterococcus faecalis and 10 parts of oligosaccharide.
[0095] The live cell count of each strain in the compound microbial feed additive is: Aspergillus niger spore number 4*10 8 cfu / g, Bacillus subtilis 10*10 9 cfu / g, Enterococcus faecalis 5*10 9 cfu / g, total bacterial count 3*10 9 cfu / g.
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Abstract
The invention provides a compound microorganism feed additive which comprises aspergillus niger, bacillus subtilis, enterococcus faecalis and oligosaccharide with the mass faction ratio being 50-60:10-20:10-20:5-10. According to the strain living cell counts, the count of aspergillus niger spores is (1-10)*10<8> cfu / g, the count of bacillus subtilis is (1-10)*10<9> cfu / g, the count of enterococcus faecalis is (5-10)*10<9> cfu / g, and the total bacteria count is not smaller than 3*10<9> cfu / g. The compound microorganism feed additive has the good disease resistance and can enhance the animal immunity. Due to the fact that the oligosaccharide is added, the animal immunity can be improved, and infectious diseases are prevented. The compound microorganism feed additive is obtained in a solid fermentation mode, the production technology and equipment are simple, operation is easy, the source of production raw materials is wide, and the price is low, so that the production cost is greatly lowered, waste materials are recycled, environmental pollution is reduced, and sustainable development is achieved. Due to the application of the compound microorganism feed additive, the piglet disease resistant ability is remarkably improved, and the death rate is decreased. The compound microorganism feed additive is of great importance in promoting sustainable development of poultry farming.
Description
technical field [0001] The invention relates to the technical field of biological fermentation, in particular to a compound microbial feed additive and a preparation method thereof. Background technique [0002] At present, the widely used feed additives in my country are medicinal feed additives. Antibiotics or other chemical drugs are often added to medicinal feed additives to inhibit the growth of pathogenic microorganisms and achieve disease resistance. However, the use of antibiotics and other drugs not only kills pathogenic microorganisms, but also greatly damages the normal microorganisms in the gastrointestinal tract of animals, resulting in an imbalance of intestinal flora and a decrease in immunity. Long-term use of medicinal feed additives will lead to the emergence of drug-resistant strains of pathogenic microorganisms, which is not conducive to the complete elimination of pathogenic bacteria. In addition, the use of medicinal feed additives is likely to produce...
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