Autotrophic and heterotrophic compound ore-leaching flora FIM-Z4 and application thereof

A technology of FIM-Z4, acidophilic heterotrophic bacteria, applied to autotrophic and heterotrophic composite leaching bacteria group FIM-Z4 and its application field, can solve the problem of low leaching efficiency and slow growth of autotrophic Thiobacillus And other issues

Active Publication Date: 2015-12-30
FUJIAN INST OF MICROBIOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Autotrophic Thiobacilli generally grow slowly, whi

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Example 1: Enrichment and domestication of autotrophic and heterotrophic compound ore leaching flora FIM-Z4

[0012] Take a low-grade copper sulfide ore sample collected from a copper sulfide mine in Fujian, add it to 9K-S medium, 32°C, 150rpm shaking culture, wait for the culture medium to turn brownish red, and observe under the microscope that there are a large number of motile bacteria. Obtain a passage 1 enrichment culture. Subculture the enriched culture of the first generation, the method is the same as above, but the amount of bacterial inoculum should be reduced generation by generation, and after 8 to 10 generations of subculture, the final enriched culture is obtained.

[0013] The preparation method of the 9K-S medium is as follows: liquid A: (NH 4 ) 2 SO 4 3.0g, K 2 HPO 4 0.5g, KCl0.1g, MgSO 4 .7H 2 O0.5g, Ca(NO 3 )0.01g, added to 800mL deionized water, H 2 SO 4 Adjust the pH to 2.0, autoclave at 121°C for 20 minutes to obtain liquid A; liquid B: ...

Embodiment 2

[0015] Example 2: Composition analysis of autotrophic and heterotrophic composite ore leaching flora FIM-Z4 bacterial population

[0016] Illumina MiSeq high-throughput sequencing technology was used to analyze the microbial community structure of the domesticated autotrophic and heterotrophic leaching flora FIM-Z4. The main steps are: using CTAB / NaCl method to extract the total DNA of autotrophic and heterotrophic compound leaching bacteria FIM-Z4, using 1% agarose gel electrophoresis to detect the integrity of the extracted genomic DNA, and amplifying bacterial 16SrRNA In the V3 region, high-throughput sequencing was carried out through the Illumina Miseq sequencing platform, high-quality sequences were extracted, and bioinformatics analysis was performed on the sequences to obtain the community structure and abundance. The results showed that the dominant bacteria of the autotrophic and heterotrophic leaching bacteria group FIM-Z4 were mainly Acidithiobacillus ferrooxidans ...

Embodiment 3

[0018] Embodiment 3: the leaching test of low-grade copper sulfide ore

[0019] A low-grade copper sulfide ore was used as a sample, the particle size of the sample was <0.075mm, the main mineral components were chalcopyrite, bornite, pyrite and galena, and the copper grade was 0.47%.

[0020] The autotrophic and heterotrophic compound leaching bacteria group FIM-Z4 (that is, the mixture of the above-mentioned Acidithiobacillus ferrooxidans, Acidithiobacillusthiooxidans, Acidiphiliuacidophilum) Culture FIM-Z4) and control bacteria (mixed bacteria of acidophilic Thiobacillus ferrooxidans and acidophilic Thiobacillus thiooxidans, the content of the two in the mixed bacteria was the same as that of autotrophic and heterotrophic compound leaching bacteria group FIM- The contents of Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans in Z4 are the same) were inserted into 9K-S medium (same as Example 1) respectively, 32 DEG C, 150rpm microscope observation and cell cou...

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Abstract

The invention discloses autotrophic and heterotrophic compound ore-leaching flora FIM-Z4 and application thereof and provides a mixed culture FIM-Z4 of acidithiobacillus ferrooxidans, acidithiobacillus thiooxidans and acidiphiliu acidophilum; the preservation number of the mixed culture FIM-Z4 is CGMCC No.11190. Through synergistic effect, the leaching reaction kinetics is improved obviously, the reaction speed is increased, the leaching period is shortened, and the leaching rate and leaching yield of metal (copper and nickel) ions in target ore are increased. The heterotrophic compound ore-leaching flora FIM-Z4 is a compatible whole, and the population ecology keeps relatively stable in a long-term domestication process. The heterotrophic compound ore-leaching flora FIM-Z4 is applied to biological metallurgy and has a better leaching effect on copper ions in low-grade copper sulphide ore and nickel ions in low-grade nickel ore.

Description

Technical field: [0001] The invention belongs to the field of metallurgy, and in particular relates to an autotrophic and heterotrophic composite ore leaching bacterial group FIM-Z4 and an application thereof. Background technique: [0002] Biological hydrometallurgy is a process in which useful metals in ores are leached through the direct, indirect or combined action of microorganisms on ores. Compared with traditional metallurgical technology, biometallurgy has the advantages of low cost, simple process flow, and environmental friendliness, and can process low-grade ore, raw ore and tailings that cannot or are difficult to process by traditional metallurgical methods. In order to adapt to different ore types and improve the efficiency of bacterial leaching, it is the key to carry out basic research and engineering application of biometallurgy to discover, collect and screen efficient, adaptable and selective leaching bacteria. [0003] At present, the microorganisms used...

Claims

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Application Information

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IPC IPC(8): C12N1/20C22B3/18C22B15/00C22B23/00C12R1/01
CPCY02P10/20
Inventor 聂毅磊陈宏罗立津贾纬
Owner FUJIAN INST OF MICROBIOLOGY
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