Preparation method of liquid fermentation spawns for industrial cultivation of agrocybe aegirit

A technology of liquid fermentation and tea tree mushroom, which is applied in the direction of fungi, etc., to achieve the effects of neat fruiting, convenient inoculation, and simple preparation methods

Inactive Publication Date: 2016-01-13
江苏华绿生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is still a blank in the research on liquid strains of tea tree mushroom industrialized cultivation

Method used

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  • Preparation method of liquid fermentation spawns for industrial cultivation of agrocybe aegirit

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Experimental program
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Effect test

Embodiment 1

[0017] Preparation of liquid fermentation strains

[0018] Obtain the solid strain of tea tree mushroom, activate and expand it into a test tube (the medium in the test tube is a solid PDA medium: 200g of potatoes (peeled), 20g of glucose, 20g of agar, 1000mL of purified water, natural pH), and placed in a 25°C Cultivate in a biochemical incubator, subculture several times in succession to obtain a stable test tube strain; transfer the stable test tube strain to a petri dish (medium is solid PDA formula: potato (peeled) 200g, glucose 20g, agar 20g , purified water 1000mL, pH natural) for activation, placed in a biochemical incubator at 25°C for cultivation, and can be used when the aerial hyphae grow to the edge of the culture dish; use a puncher to remove the grown culture dish Transfer the inoculated block to a triangular flask containing liquid medium (10g wheat flour, 10g white sugar, 2g yeast powder, 1g magnesium sulfate, 1g potassium dihydrogen phosphate, 1000mL purifi...

Embodiment 2

[0037] Preparation of liquid fermentation strains

[0038]Obtain the solid strain of tea tree mushroom, activate and expand it into a test tube (the medium in the test tube is a solid PDA medium: 200g of potatoes (peeled), 20g of glucose, 20g of agar, 1000mL of purified water, natural pH), and placed in a 25°C cultured in a biochemical incubator. Successive subculture several times to obtain a stable test tube strain; transfer the stable test tube strain to a petri dish (medium is solid PDA formula: 200g potato (peeled), 20g glucose, 20g agar, 1000mL purified water, natural pH ) for activation, placed in a biochemical incubator at 25°C for cultivation, and can be used when the aerial hyphae grow to the edge of the petri dish; use a puncher to transfer the inoculum block from the grown petri dish to the liquid-containing Medium (10g of bran, 10g of white sugar, 2g of peptone, 1g of magnesium sulfate, 1g of potassium dihydrogen phosphate, 1000mL of purified water, pH5.8-6.0) ...

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Abstract

The invention discloses a preparation method of liquid fermentation spawns for industrial cultivation of agrocybe aegirit. The preparation method includes: activating solid spawns of the agrocybe aegirit, taking original inoculation blocks after aerial mycelia grow to positions 2-3cm away from the original inoculation blocks, inoculating the inoculation blocks into a shake flask containing a liquid culture medium, performing shaking culture till mycelium pellets appear, inoculating liquid spawns into a fermentation medium of a fermentation tank, feeding clean air at the temperature of 20-25 DEG C and culturing for 8-10 days so as to obtain the liquid fermentation spawns for industrial cultivation of the agrocybe aegirit. The preparation method is simple, production cycle is short, and the spawns are high in purity, strong in vitality, quick to germinate and suitable for extensive popularization and application.

Description

technical field [0001] The invention relates to the field of preparation of liquid strains of edible mushrooms, in particular to a method for preparing liquid fermentation strains of tea tree mushroom industrialized cultivation. Background technique [0002] Tea Tree Mushroom is rich in nutrition, delicious, crisp and tender stipe, and has an excellent taste. It is a well-known edible fungus and has high medicinal value. It is called "Chinese God Mushroom". In recent years, the market demand has increased rapidly. The artificial domestication and cultivation of tea tree mushroom took a very short time. Although the output of artificial cultivation has increased greatly in recent years, due to its long harvest cycle and relatively cumbersome process, it is still in the primary stage of bag cultivation. The impact is great, and large-scale factory cultivation has not been realized, and the strains are still traditional solid strains, and the production efficiency is very low...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14
Inventor 林瑞虾余养朝张光忠邱昌里唐利华
Owner 江苏华绿生物科技股份有限公司
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