A Morganella strain and its application in microbial fuel cells
A fuel cell and Morgan bacteria technology, applied in the field of marine microorganisms, can solve the problem of scarcity of electricity-producing microorganisms, and achieve the effect of strong environmental adaptability and strong electrochemical activity
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Embodiment 1
[0029] 1. Screening of Morganella sp. DS4020
[0030] (1) Prepare the inoculum. Deep-sea sediments collected by China Ocean-1 scientific research vessel at a water depth of 4020m in the Indian Ocean. The sample is white, with fragments of chimneys and the remains of large organisms such as corals. Before screening for electrogenic bacteria, the samples were frozen and stored in a -20°C refrigerator. In the ultra-clean bench, take a small amount of seabed sludge into a 50mL centrifuge tube, add an appropriate amount of M6 diluted sludge, mix evenly with ultrasound, and centrifuge to remove solid particles, and the supernatant is the inoculum. M6 base fluid, including (g / L): 19.89NaCl, 0.745 KCl, 0.35 NaH 2 PO 4 ,0.44 Na 2 HPO 4 and 0.188 MgSO 4 , 10mL of trace elements, pH 6.8~7.0, the composition of trace elements is as follows (g / L): FeSO 4 ·7H 2 O 0.1; CoCl 2 ·6H 2 O 0.1; CaCl 2 2H2 O 0.1; ZnSO 4 ·7H 2 O 0.1; CuSO 4 ·5H 2 O 0.01; AlK(SO 4 ) 2 12H 2 O 0.01; ...
Embodiment 2
[0036] This example discloses the extracellular electron transport properties of Morganella sp. DS4020. As stated in Example 1, Morganella sp. DS4020 was inoculated into the anode compartment of the microbial fuel cell, and the microbial fuel cell was operated until the voltage reached a maximum value and remained stable. The graphite anode was taken out and put into the electrolytic cell for cyclic voltammetry scanning analysis. The potentiostat uses CHI 660E), the saturated calomel electrode is the reference electrode, and the platinum electrode (10mm×20mm) is the auxiliary electrode. The scanning voltage is -0.6~0.8V, the scanning rate is 100mV / s gradient scanning, the results are as follows Figure 4 shown. Figure 4 The cyclic voltammetry test method of the anolyte in the medium is as follows: when the voltage of the microbial fuel cell reaches the maximum value and remains stable, the anolyte is poured out, the cells in the anolyte are removed by high-speed centrifugat...
Embodiment 3
[0040] This embodiment discloses the electricity production characteristics of deep-sea electrogenic bacteria using different carbon sources. Test according to step 5 in Example 1. Coulombic efficiency calculation method formula: Among them, Cp represents the total charge obtained over a period of time, and Cn represents the total charge transferred by the oxidized substrate in theory. , where, i is the current, N is the electron transfer number of substrate oxidation, n (mmol / L) is the substrate concentration, V (L) is the solution volume in the anode chamber. The experimental results show that Morganella sp. DS4020 can use formic acid, glucose, pyruvic acid, lactic acid, mannose, sucrose, etc. to produce electricity, but the coulombic efficiency of electricity production is different. Among them, formic acid and lactic acid have the highest efficiency, almost reaching 100%, and the conversion efficiency of pyruvic acid, mannose, and sucrose is relatively low. This exampl...
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