A device for immobilized enzyme preparation/reaction and its application
A technology of immobilized enzymes and reactions, applied in enzymology/microbiology devices, enzyme production/bioreactors, biochemical cleaning devices, etc., can solve problems such as difficult molding, low production efficiency, and inability to realize large-scale production. Achieve the effect of high molding rate and preparation efficiency, reduce input cost, and significant practical value
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Embodiment 1
[0043] Embodiment 1: Apply the device of the present invention to prepare immobilized enzyme by embedding method
[0044] 1) Put sodium alginate and water into the pressure tank 1 according to the ratio through the feed port 3, turn on the stirring device 7 and the heater, and stir at 50-60°C until the sodium alginate is completely dissolved to obtain an aqueous solution of sodium alginate (solution final concentration is 1wt%);
[0045] 2) After the sodium alginate aqueous solution is cooled to room temperature, add a proportioned amount of β-glucosidase powder (5U / mL sodium alginate aqueous solution), seal the feed inlet, and stir to prepare the β-glucosidase gel ;
[0046] 3) Open the feed port to keep the inside of the tank under normal pressure, open the valve A10, let the β-glucosidase gel flow out from the distributor 9, and drop it into a container placed 20 to 40 cm directly below the distributor 9. (1~3) in the container of wt% calcium chloride aqueous solution, to...
Embodiment 2
[0049] Embodiment 2: Application of the device of the present invention to prepare immobilized enzyme by cross-linking embedding method
[0050] 1) Put sodium alginate and water into the pressure tank 1 according to the ratio through the feed port 3, turn on the stirring device 7 and the heater, and stir at 50-60°C until the sodium alginate is completely dissolved to obtain an aqueous solution of sodium alginate (solution final concentration is 3wt%);
[0051] 2) After the sodium alginate aqueous solution is cooled to room temperature, add a proportioning amount of β-glucosidase powder (5U / mL sodium alginate aqueous solution) and glutaraldehyde (to make the final concentration 0.5vol%), and seal the feed Mouth, stirring, cross-linking reaction at room temperature for 2 hours, prepared β-glucosidase gel;
[0052] 3) Open the valve A10, and at the same time, pass an appropriate amount of gas from the compressed gas inlet, so that the β-glucosidase gel liquid flows out quickly f...
Embodiment 3
[0055] Embodiment 3: Applying the device of the present invention to carry out hydrolysis reaction of immobilized enzyme in single-phase system
[0056] a) Put immobilized enzyme, baicalin, and acetic acid-sodium acetate buffer solution with pH=4.0 to 6.0 into the pressure-resistant tank 1 according to the proportion (20:1:500, weight ratio) through the feed port 3, and seal the reaction system , turn on the stirring device 7 and the heater, and stir at 40-60° C. to carry out the enzymolysis reaction;
[0057] b) After the reaction is finished, open the feed inlet to keep the reaction system under normal pressure, then open the valve B12 to release the reaction solution.
[0058] As a preferred option, a sieving device (such as a screen) is placed at the bottom of the pressure tank 1 to screen out the immobilized enzyme particles. After the reaction solution is completely released, the flange cover 2 can be opened and the screen Take it out to get the collected immobilized en...
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Abstract
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