Process for enzymatic synthesis of (R)-4-cyano-3-hydroxybutyrate from sodium alginate immobilized halogenohydrin dehalogenase

A technology of ethyl hydroxybutyrate and halohydrin dehalogenase, applied in the field of medicine and chemical industry, can solve the problems of side reactions, many influencing factors, difficult to control, etc., and achieve the effects of high immobilization rate, high catalytic efficiency and fast immobilization rate.

Inactive Publication Date: 2016-02-10
JIANGSU UNIV OF TECH
View PDF3 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The microbial conversion method is to use the biological system or its multi-enzyme or single-enzyme system to catalyze the target substrate to convert one substance into another substance. The principle is to use enzymes to catalyze a special reaction; Various microorganisms, these microorganisms are rich in active enzymes and have strong vitality. Therefore, for the preparation of chiral substances, microbial tra...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Process for enzymatic synthesis of (R)-4-cyano-3-hydroxybutyrate from sodium alginate immobilized halogenohydrin dehalogenase
  • Process for enzymatic synthesis of (R)-4-cyano-3-hydroxybutyrate from sodium alginate immobilized halogenohydrin dehalogenase
  • Process for enzymatic synthesis of (R)-4-cyano-3-hydroxybutyrate from sodium alginate immobilized halogenohydrin dehalogenase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A kind of technology that sodium alginate immobilizes halohydrin dehalogenase and catalyzes synthesis (R)-4-cyano-3-hydroxybutyric acid ethyl ester, comprises the following steps:

[0028] 1) Extract 15U / mL halohydrin dehalogenase solution from the crude halohydrin dehalogenase solution for use; prepare a sodium alginate aqueous solution with a mass fraction of 3.5wt% for use; prepare a volume fraction of 1% glutaraldehyde Aqueous solution ready for use;

[0029] 2) Drop 2 mL of glutaraldehyde aqueous solution into a uniformly mixed solution of 40 mL of sodium alginate aqueous solution and 40 mL of halohydrin dehalogenase solution, and react for 5 hours under stirring conditions to obtain a stationary solution;

[0030] 3) Drop the fixative solution into 100 mL of 2 wt % calcium chloride aqueous solution drop by drop to form bead beads, solidify for 1 hour and filter to obtain sodium alginate immobilized halohydrin dehalogenase;

[0031] 4) Weigh 207g of distilled wate...

Embodiment 2-9

[0036] Each embodiment is basically the same as embodiment 1, the difference lies in table 1.

[0037] Table 1

[0038]

[0039]

[0040] Table 2 shows the immobilization rate of halohydrin dehalogenase and its ATS-5 production rate after immobilization in each embodiment.

[0041] Table 2

[0042]

[0043] Note: where "the first time" is the generation rate of directly using the halohydrin dehalogenase immobilized in sodium alginate to catalyze the synthesis of (R)-4-cyano-3-hydroxybutyric acid ethyl ester, "the second "Times" is the generation rate of sodium alginate immobilized halohydrin dehalogenase used to catalyze the synthesis of ethyl (R)-4-cyano-3-hydroxybutyrate after the completion of the first synthesis.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a process for enzymatic synthesis of (R)-4-cyano-3-hydroxybutyrate from sodium alginate immobilized halogenohydrin dehalogenase. The process comprises the following steps: firstly, mixing sodium alginate with HHDH, cross-linking with glutaraldehyde, embedding by using calcium chloride, immobilizing for 1 hour, after the immobilization rate of HHDH is greater than 46%, enabling immobilized enzyme and a substrate (S)-4-chlorine-3-hydroxybutyrate (ATS-4) to react for 4 hours till the generation rate of ATS-5 is not less than 70%, recycling HHDH, and performing secondary catalytic reaction, wherein the generation rate of ATS-5 is not less than 35%. By adopting the method of sodium alginate immobilized halogenohydrin dehalogenase, the immobilization velocity of sodium alginate to halogenohydrin dehalogenase is rapid, the immobilization rate is high, and after the sodium alginate immobilized halogenohydrin dehalogenase is recycled after first enzymatic synthesis of (R)-4-cyano-3-hydroxybutyrate, relatively high catalytic efficiency can be stilled maintained, and the immobilization effect is good.

Description

technical field [0001] The invention relates to a preparation method of an atorvastatin intermediate, in particular to a process for catalyzing the synthesis of (R)-4-cyano-3-hydroxybutyric acid ethyl ester by immobilizing halohydrin dehalogenase with sodium alginate, which belongs to medicine chemical industry. Background technique [0002] Scientists have discovered that statins not only have a powerful lipid-lowering effect, but also have multiple effects. They can improve intravascular cardiomyocyte remodeling and inhibit cardiac electrophysiological remodeling, prevent cardiomyocyte apoptosis and regulate cardiac autonomic function, etc. . The emergence of statins is an important milestone in the history of cardiovascular disease treatment, and it has become an assistant blood lipid regulating drug that is currently clinically used for all kinds of hyperlipidemia, prevention and treatment of atherosclerosis and coronary heart disease, and is also a well-known drug in t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12P13/00C12N11/10C12N11/04
Inventor 梁国斌汪斌周全法姚佳
Owner JIANGSU UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap