Honeysuckle tissue-culture-seedling culture medium and honeysuckle tissue-culture rapid propagation method

A honeysuckle group and culture medium technology, applied in the field of plant tissue culture, can solve the problems of not being able to meet the needs of honeysuckle seedlings, low survival rate, excellent plant characteristics and quality reduction, etc.

Inactive Publication Date: 2016-02-24
SHANDONG UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, at present, honeysuckle generally adopts cutting, grafting, branching or layering methods of propagation, which are not only limited by seasons, but also have a low survival rate, which cannot meet the needs of the honeysuckle planting industry for seedlings
And with the increase of reproduction algebra, the excellent characteristics and quality of plants will continue to decrease

Method used

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  • Honeysuckle tissue-culture-seedling culture medium and honeysuckle tissue-culture rapid propagation method
  • Honeysuckle tissue-culture-seedling culture medium and honeysuckle tissue-culture rapid propagation method
  • Honeysuckle tissue-culture-seedling culture medium and honeysuckle tissue-culture rapid propagation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The best tissue culture rapid propagation method of Lonicera, comprising the following steps:

[0030] Step 1, the preparation of several MS medium mother solutions.

[0031] MS100 times the preparation of a large amount of element mother liquor, respectively weighed 190g of potassium nitrate, 165g of ammonium nitrate, 17g of potassium dihydrogen phosphate, 37g of magnesium sulfate, and 44g of calcium chloride, respectively added to five 1000mL beakers, added an appropriate amount of distilled water, stirred to dissolve, and fixed The capacity is 1000mL, and the five kinds of macroelement mother liquors are stored in the refrigerator for later use.

[0032] Preparation of MS1000 times trace element mother liquor, weigh 0.83g of potassium iodide, 6.2g of boric acid, 16.9g of manganese sulfate, 8.6g of zinc sulfate, 0.25g of sodium molybdate, 0.025g of copper sulfate, and 0.025g of cobalt chloride into a 1000mL beaker , add an appropriate amount of distilled water, stir ...

Embodiment 2

[0042] This patent has successively investigated the effects of different sterilization times, sterilization concentrations and sterilization methods, different hormones and their concentrations, and different temperatures in the culture conditions on the tissue cultured plantlets of Lonicera Lonicerae (except for the investigation factors, other factors and conditions are equivalent to the implementation Example 1), determined the optimum culture scheme of Lonicera tissue culture seedling. The results of the investigation are listed below.

[0043] Table 1 The sterilization effect of different sterilization time, sterilization concentration and sterilization method on Lonicera explants

[0044]

[0045] It can be seen from Table 1 that the effect of sterilizing explants with only 75% alcohol is not ideal, so consider using sodium hypochlorite after alcohol sterilization. By further exploring and shortening the test time as much as possible, it can be seen that after steri...

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Abstract

The invention discloses a tissue-culture-seedling culture medium and a honeysuckle tissue-culture rapid propagation method. A honeysuckle is a species of the honeysuckle family and a woody perennial and semi-evergreen trailing honeysuckle and has the multiple pharmacological activities of bacterium resisting, virus resisting, heat removing, inflammatory resisting and the like. The main reproductive mode of the honeysuckle at present is cutting propagation, the survival rate is low, the reproduction coefficient is small, and the large-scale production requirements can not be met; according to the tissue-culture-seedling culture medium and the honeysuckle tissue-culture rapid propagation method, honeysuckle branches with axillary buds serve as materials, a honeysuckle tissue-culture rapid-propagation technical system is built through the steps of explant disinfection treatment, induction culturing, rooting culturing, acclimatization, transplanting and the like, and technical supports are provided for cultivating more high quality seedlings in a short time period to meet the market requirements.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, in particular to a tissue culture technology of honeysuckle. Background technique [0002] Honeysuckle, also known as golden flower, silver flower, honeysuckle, double flower, mandarin duck vine, etc., is a perennial twining vine of the family Lonicera. It is often used as medicine with its flowers, leaves and stems. It is a traditional Chinese medicine commonly used in my country. Honeysuckle is sweet in taste and cold in nature. It has the effects of clearing away heat and detoxification, and dispersing wind-heat. Honeysuckle mainly contains organic acid flavonoids and volatile oils, which have the functions of antibacterial and anti-inflammatory, anti-tumor, liver protection, hemostasis, lowering blood fat, lowering blood sugar, anti-fertility, and hangover. [0003] Honeysuckle has a wide range of uses. On the one hand, as a common medicinal material, it is used in clinics and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 蒲高斌张永清梁从莲李佳刘谦
Owner SHANDONG UNIV OF TRADITIONAL CHINESE MEDICINE
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