Honeysuckle tissue-culture-seedling culture medium and honeysuckle tissue-culture rapid propagation method
A honeysuckle group and culture medium technology, applied in the field of plant tissue culture, can solve the problems of not being able to meet the needs of honeysuckle seedlings, low survival rate, excellent plant characteristics and quality reduction, etc.
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Embodiment 1
[0029] The best tissue culture rapid propagation method of Lonicera, comprising the following steps:
[0030] Step 1, the preparation of several MS medium mother solutions.
[0031] MS100 times the preparation of a large amount of element mother liquor, respectively weighed 190g of potassium nitrate, 165g of ammonium nitrate, 17g of potassium dihydrogen phosphate, 37g of magnesium sulfate, and 44g of calcium chloride, respectively added to five 1000mL beakers, added an appropriate amount of distilled water, stirred to dissolve, and fixed The capacity is 1000mL, and the five kinds of macroelement mother liquors are stored in the refrigerator for later use.
[0032] Preparation of MS1000 times trace element mother liquor, weigh 0.83g of potassium iodide, 6.2g of boric acid, 16.9g of manganese sulfate, 8.6g of zinc sulfate, 0.25g of sodium molybdate, 0.025g of copper sulfate, and 0.025g of cobalt chloride into a 1000mL beaker , add an appropriate amount of distilled water, stir ...
Embodiment 2
[0042] This patent has successively investigated the effects of different sterilization times, sterilization concentrations and sterilization methods, different hormones and their concentrations, and different temperatures in the culture conditions on the tissue cultured plantlets of Lonicera Lonicerae (except for the investigation factors, other factors and conditions are equivalent to the implementation Example 1), determined the optimum culture scheme of Lonicera tissue culture seedling. The results of the investigation are listed below.
[0043] Table 1 The sterilization effect of different sterilization time, sterilization concentration and sterilization method on Lonicera explants
[0044]
[0045] It can be seen from Table 1 that the effect of sterilizing explants with only 75% alcohol is not ideal, so consider using sodium hypochlorite after alcohol sterilization. By further exploring and shortening the test time as much as possible, it can be seen that after steri...
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