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Continuous-phase-change extraction method for bacteriostatic substance in oil tea and application thereof

A technology of antibacterial substances and extraction, applied in the field of biochemistry, to reduce production costs, reduce energy consumption, and simplify the process

Active Publication Date: 2016-03-02
ZHONGKAI UNIV OF AGRI & ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no report on the extraction of antibacterial substances in camellia oleifera by continuous phase change extraction.

Method used

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  • Continuous-phase-change extraction method for bacteriostatic substance in oil tea and application thereof
  • Continuous-phase-change extraction method for bacteriostatic substance in oil tea and application thereof
  • Continuous-phase-change extraction method for bacteriostatic substance in oil tea and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Example 1 Effect of ethanol concentration on the antibacterial activity of continuous phase change extraction of antibacterial substances in camellia oleifera

[0068] (1) Raw material processing: crush the seed dregs of camellia oleifera to 20-80 mesh to obtain tea powder raw material.

[0069] (2) Continuous phase change extraction: 1.5 kg of the tea powder raw material obtained in step (1) is packed into an extraction kettle, and the concentration is 50%, 60% , 70%, 80%, and 90% ethanol extractants are compressed into liquids, and under the conditions of extraction temperature 80°C and extraction pressure 0.4MPa, flow through the extraction kettle at a flow rate of 100L / h, and continuously extract for 2h to extract the pH in Camellia oleifera. After decomposing the bacterial substances, they flowed through the desorption kettle under the conditions of the desorption temperature of 80° C. and the desorption pressure of 0.2 MPa to obtain 287, 293, 300, 310 and 303 g of...

Embodiment 2

[0085] Example 2 The influence of extraction pressure on the antibacterial activity of antibacterial substances in Camellia oleifera extracted by continuous phase change extraction

[0086] For further illustrating the beneficial effect of the present invention, repeat the step of embodiment 1, it is 80% that the ethanol extraction agent of 50%, 60%, 70%, 80%, 90% in embodiment 1 step (2) is replaced into concentration Ethanol extractant, the extraction pressure 0.4MPa in step (2) of Example 1 is replaced by 0.3MPa, 0.4MPa, 0.5MPa, 0.6MPa, 0.7MPa, and the antibacterial substances 291, 302, 311, 301 in camellia oleifera are obtained respectively , 297g, specifically as shown in Table 2.

[0087] The experimental results show that: as can be seen from Table 2, with the gradual increase of the extraction pressure of the system, the diameter of the inhibition zone changes, but the regularity is not obvious. When the pressure reaches 0.5MPa, the extract has no effect on E. The ant...

Embodiment 3

[0090] Example 3 The influence of extraction temperature on the antibacterial activity of antibacterial substances in Camellia oleifera extracted by continuous phase change extraction

[0091] For further illustrating the beneficial effect of the present invention, repeat the step of embodiment 1, it is 80% that the ethanol extraction agent of 50%, 60%, 70%, 80%, 90% in embodiment 1 step (2) is replaced into concentration Ethanol extractant, replace the extraction temperature 80°C in step (2) of Example 1 with 50°C, 60°C, 70°C, 80°C, and 90°C to obtain antibacterial substances 293, 296, 302, and 317 in Camellia oleifera, respectively , 310g, specifically as shown in Table 3.

[0092] The experimental results show that: as can be seen from Table 3, with the gradual increase of the extraction temperature, the inhibitory zone of the extract against Escherichia coli, Staphylococcus aureus and Aspergillus niger increases, and when the temperature exceeds 80°C, it shows a gradual down...

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Abstract

The invention provides a continuous-phase-change extraction method for a bacteriostatic substance in oil tea and an application thereof, and also provides an oil tea bacteriostatic extract product. The employed continuous-phase-change extraction method has the advantages of simple process flow and strong operationality, can effectively reduce the production cost, shortens the extraction separating time, and reduces energy consumption in the technical process; the method has the advantages of safety, environmental protection, no pollution and no harmful solvent residues, and can realize large-scale industrial production. The bacteriostatic substance in the oil tea and a purified bacteriostatic extract product can reach good antibacterial effect.

Description

technical field [0001] The invention relates to the technical field of biochemistry, in particular to a method for extracting antibacterial substances in camellia oleifera by continuous phase change extraction and its application, and also relates to an antibacterial extract of camellia oleifera. Background technique [0002] The research and application of the extraction of active ingredients in natural product raw materials has a history of many years, and some products have also been applied on a large scale in industry. In the current scientific research, the separation of active ingredients of natural products still uses various solvents of different polarities. According to the principle of "like dissolves like", for the target extract, select the ingredients that have high solubility for the required antibacterial active ingredients and those that do not need to be dissolved. The solvent with low solubility dissolves the target component from the raw material, and the...

Claims

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Application Information

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IPC IPC(8): A01N65/08C07H1/06C07H17/065A01N43/16A01P1/00A01P3/00
CPCA01N43/16A01N65/08C07H1/06C07H17/065
Inventor 丘苑新曹庸翟万京赵丽柳建良
Owner ZHONGKAI UNIV OF AGRI & ENG
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