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Antibacterial material and preparation and application thereof

A technology of antibacterial materials and antibody molecules, applied in the field of preparation of antibacterial materials, to achieve the effect of simple preparation method, low price and superior biocompatibility

Active Publication Date: 2016-03-09
SHANGHAI NINTH PEOPLES HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, no ideal material has been found that has the two functions of antibacterial and remineralization, and comprehensively prevents dental caries like fluoride.

Method used

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  • Antibacterial material and preparation and application thereof
  • Antibacterial material and preparation and application thereof
  • Antibacterial material and preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] 1. Preparation of hydroxyapatite:

[0048] At room temperature, 1.791 g of Na 2 HPO 4 ·12H 2 O was dissolved in 250 ml of deionized water to form solution A, and 1.762 g of Ca(CH 3 COO) 2 ·H 2 O was dissolved in 100 mL of deionized water to form solution B. Add 0.030 g of protidine DNA to 30 ml of solution A, stir at room temperature to dissolve it completely, adjust the pH to 5 with 1 mol / L of hydrochloric acid, then add 10 ml of solution B dropwise, and use 1 mol / L of liters of hydrochloric acid to adjust the pH and keep it at around pH 5. After the dropwise addition was complete, the mixed clear liquid was transferred into a Teflon liner (liner capacity 100 ml) and sealed. Put the lining into the hydrothermal reaction jacket and put the jacket together with the microwave reaction oven to react at 120° C. for 10 minutes. After the reaction system was naturally cooled to room temperature, the product was taken out, and the product was separated by centrifugatio...

Embodiment 2

[0056] 1. Preparation of hydroxyapatite:

[0057] At room temperature, 1.791 g of Na 2 HPO 4 ·12H 2 O was dissolved in 250 ml of deionized water to form solution A, and 1.762 g of Ca(CH 3 COO) 2 ·H 2 O was dissolved in 100 mL of deionized water to form solution B. Add 0.030 g of protidine DNA to 30 ml of solution A, stir at room temperature to dissolve it completely, add 10 ml of solution B, and adjust the pH to 10 with 1 mol / L of sodium hydroxide. Transfer the mixed clear liquid into a Teflon liner (liner capacity 100 ml) and seal. Put the lining into the hydrothermal reaction jacket and put the jacket together with the microwave reaction oven to react at 120° C. for 10 minutes. After the reaction system was naturally cooled to room temperature, the product was taken out, and the product was separated by centrifugation. The separated product was washed three times with deionized water, once with absolute ethanol, and dried in the air at 60°C to obtain the product shown...

Embodiment 3

[0066] Antibacterial effect experiment

[0067] 1. Experimental materials

[0068] Three common pathogenic bacteria in oral caries and periodontal disease: Streptococcus mutans (UA159), Lactobacillus acidophilus (L.acidophilus, ATCC4356) and Fusobacterium nucleatum (Fusobacterium nucleatum, ATCC25586) were selected as samples to inhibit bacteria Experimental strains.

[0069] 2. Experimental method

[0070] Streptococcus mutans and Fusobacterium nucleatum were resuscitated and cultured in bovine brain infusion (Brain Heart Infusion, BHI), and Lactobacillus acidophilus was cultured in MRS medium. After the bacteria were recovered, they were cultured in an anaerobic box on a solid medium for 48 hours, and independent colonies were picked and placed in a liquid medium, and cultured anaerobically for 24 hours. Take the bacterial suspension in the logarithmic growth phase of the third generation as the experimental bacterial liquid.

[0071] First select the ones prepared in Ex...

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Abstract

The invention belongs to the field of preparation of antibacterial materials and particularly relates to an antibacterial material and preparation and application thereof. The antibacterial material contains hydroxyapatite and IgY antibody molecules, wherein the mass ratio of the IgY antibody molecules to the hydroxyapatite is (0.001-5):1. The IgY antibody molecules are supported on the hydroxyapatite for the first time to form the brand new antibacterial material. The IgY antibody molecules supported on the hydroxyapatite have good activity, can give full play to its antibacterial activity and have the obvious bactericidal effect on streptococcus mutans. It is found that the antibacterial material formed by supporting the IgY antibody molecules on the hydroxyapatite has the effect of inhibiting lactobacillus acidophilus for the first time.

Description

technical field [0001] The invention belongs to the field of antibacterial material preparation, in particular to an antibacterial material and its preparation and application. Background technique [0002] Caries is a common and frequently-occurring disease in the oral cavity, and fluoride is the most studied and widely used anti-caries method. However, in many areas of my country, the fluoride content in water exceeds the standard, and chronic fluorosis symptoms such as dental fluorosis and bone fluorosis will occur if excessive fluoride intake is long-term. Therefore, finding a safe and effective fluoride-free anti-caries material is an important topic in the stomatology community at home and abroad. [0003] Calcium phosphates are the most important inorganic components in human hard tissues. Due to their excellent physicochemical and biological properties, the preparation and application of calcium phosphate materials has always been a hot spot in medical research fiel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/395A61P1/02A61P31/04A61K33/42
CPCA61K33/42A61K39/395A61K2300/00
Inventor 陈曦陈峰杨彬冯希平
Owner SHANGHAI NINTH PEOPLES HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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