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Penicillium griseofulvum for preventing and treating fungal disease and application thereof

A technology for Penicillium griseum and sclerotinia, which is applied in the directions of application, fungi, fungicides, etc., to prevent the expansion of disease spots, low cost, and simple production and use.

Active Publication Date: 2016-03-09
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the prevention and control of gray mold by Penicillium griseus

Method used

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  • Penicillium griseofulvum for preventing and treating fungal disease and application thereof
  • Penicillium griseofulvum for preventing and treating fungal disease and application thereof
  • Penicillium griseofulvum for preventing and treating fungal disease and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Isolation and Identification of P417 Strain

[0043] 1. Isolation location, isolation method (morphological identification of traditional microbiology)

[0044] Microbial characteristics

[0045] Isolation of P417 strain: The applicant isolated and screened a Penicillium strain with strong control effect on Botrytis cinerea from rape plants collected in Qinghai Province, China, and the applicant named it P417. The separation and screening of P417 bacterial strain of the present invention adopt methods such as dilution plate method, plate antagonism, in vitro test, strain identification work with reference to " fungus identification handbook " (Wei Jingchao, 1979), " morphology and classification of fungi " (Dai Fanglan, 1987) ) and other monographs of the test methods. The P417 strain was cultured at 25°C for 5 days, and the genomic DNA was extracted by the CTAB method (Tayloretal 1993), and the rDNA-ITS region was amplified by using fungal ribosomal DNA universal pri...

Embodiment 2

[0056] P417 strain and Botrytis cinerea plate confrontation test:

[0057] Use a puncher with an inner diameter of 9mm to punch the activated Botrytis cinerea and connect it to one side of the PDA plate, and the other side to connect the P417 strain (9mm inner diameter). Botrytis cinerea was inoculated and the other side was not inoculated as a control, and repeated 3 times. After 3-4 days, observe the magnitude of its antagonistic effect and measure the width of its bacteriostatic zone. See the test results Figure 4 ,Table 1.

[0058] Table 1 Inhibition rate of P417 strain against Botrytis cinerea in confrontation test (3d, 20°C)

[0059]

[0060] The results of the confrontation test are shown in Table 1: the average antibacterial width of the plate confrontation test is 2.5m, and the radius of gray mold is only 1.13cm, while in the control group, the average radius of gray mold is 3.37cm, and the average inhibition rate is more than 71.4%. , which is a strain with s...

Embodiment 3

[0062] Preliminary Analysis of the Antagonism Mechanism of Strain P417

[0063] (1) Effect of P417 fermentation product on the growth rate of Botrytis cinerea

[0064] Use a puncher with an inner diameter of 9mm to take out the P417 bacterial block, transfer it to 100mL PDB, and shake the bacteria for 5 days at 25°C and 160rpm. The prepared spore suspension was centrifuged at 8,000 r / min for 10 min, and the supernatant was suction-filtered with a 0.22 μm microporous membrane to obtain a sterile fermentation liquid, namely the fermentation supernatant. Add the fermentation supernatant to PDA at 1%, 5%, and 10% and pour it onto a plate, connect a gray mold hyphae block with a diameter of 9mm on each plate, repeat each treatment 3 times, and perform 3 parallel experiments, culture at 20°C , Measure the colony diameter of Botrytis cinerea on 3d, 5d, and 7d and record relevant data. The test result is Figure 5 ,Table 2.

[0065] The influence of table 2P417 fermentation produc...

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Abstract

The invention discloses penicillium griseofulvum for preventing and treating fungal disease and application thereof. The penicillium griseofulvum is penicillium griseofulvum (P. griseofulvum) P417 with a preservation number of CCTCC NO: M2015684. A bacterial strain has good prevention effects on gray mold and stalk break of plants; the penicillium griseofulvum P417 bacterial strain has a flat plate antagonism experiment average bacteriostasis width of 2.5cm to botrytis cinerea, has an average bacteriostasis rate of more than 70 percent, is a bacterial strain with strong antagonistic activity, has an average bacteriostasis rate of more than 54 percent to sclerotinia sclerotiorum, and is non-toxic to people and livestock; the cost is low; fermentation liquor of the penicillium griseofulvum P417 can be used for directly preventing and treating gray mold or stalk break of plants, great simpleness in production and use is realized, organic solvents are not used in the production, pollution in the production process and products is reduced, solvent poisoning is also avoided, and an advantage that a biopesticide is safe to people and livestock is achieved.

Description

technical field [0001] The invention belongs to the technical field of biological control of plant diseases, and in particular relates to Penicillium grisea for preventing and treating fungal diseases and an application thereof. Background technique [0002] Vegetables are one of the essential foods in people's daily diet. Vegetables can provide nutrients such as various vitamins and minerals necessary for the human body. Botrytis cinerea Pers. is a worldwide fungal disease caused by Botrytis cinerea Pers. The disease has generally occurred all over the country and is on the rise. It has become an important disease in vegetable production. Botrytis cinerea has a wide range of hosts, not only infecting tomatoes, but also infecting about 235 crops such as lettuce, eggplant, cucumber, pepper, strawberry, grape, garlic, and citrus. Botrytis cinerea can harm various crops such as vegetables, fruit trees and flowers, especially in the production of fruits and vegetables in prote...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/80
Inventor 肖炎农王娜王高峰杨波孟俊峰袁和奇肖雪琼吴官维
Owner HUAZHONG AGRI UNIV
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