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Test strip for detecting iprodione and preparation method and application of test strip

A technology of iprodione and test strips, which is applied in the field of test strips and preparations for detecting iprodione, can solve the problems of high detection cost and long detection time of analytical instrument methods, and achieve short detection time, simple storage, and specificity strong effect

Active Publication Date: 2016-03-09
ZHENGZHOU TOBACCO RES INST OF CNTC +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Analytical instrument method has high detection cost, requires professional and technical personnel to operate, and takes a long time to detect, which has limitations for the development of on-site detection work

Method used

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  • Test strip for detecting iprodione and preparation method and application of test strip
  • Test strip for detecting iprodione and preparation method and application of test strip
  • Test strip for detecting iprodione and preparation method and application of test strip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1, the composition of the test strip that detects iprodione

[0046] like figure 1As shown, the test strip for detecting iprodione according to the preferred embodiment of the present invention is composed of a sample absorbent pad 1 , a conjugate release pad 2 , a reaction film 3 , and a water absorbent pad 4 pasted on the bottom plate 5 in sequence. The end of the sample absorption pad is connected to the reaction membrane, the end of the reaction membrane is connected to the water absorption pad, the beginning of the sample absorption pad is aligned with the beginning of the bottom plate, and the end of the water absorption pad is aligned with the end of the bottom plate; there are detection areas 6 and Quality control area 7, the detection area and the quality control area are both strip-shaped vertical to the length of the test strip. The detection area is located on the side close to the sample absorbent pad, and the quality control area is located on ...

Embodiment 2

[0047] The preparation method of test strip described in embodiment 2, embodiment 1

[0048] 1. Preparation of Iprodione Hapten

[0049] Synthesis of Compound A: Take 5.0g of 2,6-dichloro-4-nitrophenol and 3.2g of tert-butyl 4-bromobutyrate and stir in acetone, then add 2.6g of anhydrous potassium carbonate as a catalyst and react at 60°C 8h, after the reaction stopped, the solvent was evaporated to dryness, then extracted with water and ethyl acetate, dried with anhydrous sodium sulfate, concentrated, passed through a 200-300 mesh silica gel column, separated and purified by chromatography to obtain 5.23g of compound A, with a yield of 87% . 1HNMR (CDCl3, 300MHZ) δ: 7.945(1H,d,J=0.000),4.144(2H,t,J=7.500),7.945(1H,d,J=0.000),1.973(2H,tt,J=7.500 ,J=7.367),2.359(2H,t,J=7.367),1.414(3H),1.414(3H),1.414(3H,s).

[0050] Synthesis of Compound B: Add 3.1g of zinc powder and 1mL of glacial acetic acid to 20mL of water, react at 90°C for 30min, then add 5.2g of compound A in ethano...

Embodiment 3

[0099] Embodiment 3, the detection of iprodione residue in the sample

[0100] 1. Sample pretreatment

[0101] Tobacco leaf samples need to be warmed to room temperature 20-25°C before testing; weigh 1.0±0.05g of crushed samples into a polystyrene centrifuge tube; add 10mL of sample extract (50% methanol), and use a homogenizer to fully beat After standing still, pipette 0.1mL supernatant and 0.4mL sample diluent and mix to obtain the sample solution to be tested.

[0102] 2. Test with test strips

[0103] Use a pipette to take 100 μL of the sample solution to be tested (2-3 drops from the dropper) and drop it vertically into the sample hole. When the liquid flows, start timing, react for 10 minutes, and judge the result. If it exceeds 10 minutes, the result can be used as a reference.

[0104] 3. Analyze test results

[0105] Negative (-): The color of the T line is darker than that of the C line or the color is consistent, which means that the concentration of iprodione i...

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Abstract

The invention discloses a test strip for detecting iprodione and a preparation method and application of the test strip. The test strip comprises a sample absorption cushion (1), a compound release cushion (2), a reaction film (3), a water absorption cushion (4) and a bottom plate (5). The test strip is characterized in that a detection area (6) wrapped by iprodione hapten-carrier protein conjugate and a quality control area (7) wrapped by goat anti-mouse antibodies are arranged on the reaction film, and the compound release cushion (2) is coated with iprodione monoclonal antibody-colloidal gold markers. The invention further provides a method for detecting iprodione residues in tobacco leaves through the prepared iprodione test strip. The test strip has the advantages of being easy to operate, free of sample pretreatment, high in sensitivity, high in detection speed, low in cost and the like, and is suitable for screening and on-site monitoring of a large number of samples.

Description

technical field [0001] The invention relates to the detection of iprodione, in particular to a test strip for detecting iprodione, its preparation method and its application in tobacco leaf detection. Background technique [0002] Iprodione, also known as diphenhydramine, has the molecular formula C 13 h 13 Cl 2 N 3 o 3 , belonging to dicarboximides, is a broad-spectrum contact-type protective fungicide, widely used in tobacco, fruit trees, vegetable disease control and fruit storage and preservation. Iprodione can be absorbed systemically through roots, and can effectively control fungi that are resistant to benzimidazole systemic fungicides. Its main control targets are diseases caused by Botrytis, Alternaria, and Sclerotinia, such as Botrytis cinerea, early blight, black spot, and Sclerotinia. Iprodione pesticide is a testing item with national limit requirements in Japan, the United States, South Korea, Australia, Canada and the Codex Alimentarius Commission, and i...

Claims

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Application Information

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IPC IPC(8): G01N33/558
CPCG01N33/558
Inventor 范子彦陈黎冯静崔海峰潘立宁胡斌唐纲岭刘惠民朱亮曹东山
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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