Method used for improving antioxidant activity and antibacterial activity enteromorpha polysaccharide at same time

The technology of prolifera polysaccharide and antibacterial activity is applied in the field of chemical industry, which can solve the problem of not simultaneously improving the antibacterial and antioxidant activities of prolifera polysaccharide, and achieve the effect of improving the antioxidative activity in vitro.

Active Publication Date: 2016-03-16
ZHEJIANG GONGSHANG UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

It has been reported in the literature that the sulfation, phosphorylation and acetylation of Enteromorpha polysaccharides have been modified, and the antioxidant activity of the modified Enteromorpha polysaccharides has been significantly improved; it has al

Method used

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  • Method used for improving antioxidant activity and antibacterial activity enteromorpha polysaccharide at same time
  • Method used for improving antioxidant activity and antibacterial activity enteromorpha polysaccharide at same time
  • Method used for improving antioxidant activity and antibacterial activity enteromorpha polysaccharide at same time

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Experimental program
Comparison scheme
Effect test

Embodiment 1-1

[0029] Embodiment 1-1, the degradation of Enteromorpha crude polysaccharide (EP):

[0030] Enteromorpha crude polysaccharide (EP) was dissolved in distilled water to prepare a solution of Enteromorpha crude polysaccharide with a concentration of 10mg / mL. After heating up to 30°C, hydrogen peroxide and vitamin C were added until the final concentrations of hydrogen peroxide and vitamin C were equal to 9mmol / L; then keep warm and stir for 2h, so as to realize the degradation of the crude polysaccharide of Enteromorpha enteromorpha.

[0031] Concentrate the resulting reaction solution (concentrate under reduced pressure at 60-70°C) to about 1 / 3 of the original volume to obtain a concentrated solution; use 4 times the volume concentration of the concentrated solution to precipitate with 95% ethanol, overnight at 4°C ( about 12 hours), centrifuge (8000rpm, 20min), take the precipitate and redissolve it in water (the amount ratio of the precipitate to water is about 20mg / ml), dialyz...

Embodiment 2-1

[0032] Embodiment 2-1, the carboxymethylation of degrading Enteromorpha polysaccharide:

[0033] Dissolve 0.3 g of degraded Enteromorpha polysaccharide (DEP) in 12.5 mL of DMSO, stir at room temperature for 2 h, add sodium hydroxide solution (20%, 5 mL), heat to 40° C. and stir for 3 h. Get the chloroacetic acid of 6.62g (0.07mol) and be dissolved in the NaOH solution (20%) of 5mL and the DMSO of 12.5mL, join in the above-mentioned reaction system after dissolving, thereby make the final concentration of chloroacetic acid be 2.0mol / L; ℃ for 4 hours, cooled the reaction solution to room temperature, adjusted the pH to neutral (ie, pH = 7) with 0.5 mol / L HCl, diluted with water (the volume ratio of water to the reaction solution was about 1:1), and then used Dialyze with a 3500Da dialysis bag for 48 hours, concentrate the dialysate (concentrate under reduced pressure at 60-70°C) to about 1 / 3 of the original volume, freeze-dry (freeze-dry at -50°C for 24 hours), and obtain carbox...

Embodiment 2-2

[0034] Embodiment 2-2, the carboxymethylation of degrading Enteromorpha polysaccharide:

[0035]The reaction time in embodiment 2-1 is changed into 3 hours from 4 hours, and all the other are the same as embodiment 2-1. The carboxymethyl substitution degree of carboxymethylated Enteromorpha polysaccharide obtained was 0.631.

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Abstract

The invention discloses a method used for improving antioxidant activity and antibacterial activity enteromorpha polysaccharide at the same time. The method comprises following steps: enteromorpha polysaccharide is degraded with hydrogen peroxide and vitamin C so as to obtain degraded enteromorpha polysaccharide; chloroacetic acid is taken as a carboxymethylation reagent, and is reacted with the degraded enteromorpha polysaccharide so as to obtain carboxymethylated enteromorpha polysaccharide (CDEP). The obtained CDEP possesses relatively low molecular weight (20 to 120kDa), and relatively excellent in vitro antioxidant activity and antibacterial activity, and the degree of carboxymethyl substitution (DS) ranges from 0.3 to 1.0.

Description

technical field [0001] The invention belongs to the technical field of chemistry and chemical engineering, and relates to a method of chemically modifying the degraded enteromorpha polysaccharide to enhance its biological activity. Background technique [0002] Enteromorpha is a large-scale economical green algae in the southeast coast of my country. It is rich in resources and can be eaten by itself. It also contains a variety of active substances, such as Enteromorpha polysaccharides, lipid pigments, and phenols. According to literature reports, Enteromorpha polysaccharide has physiological functions such as improving immunity of mammals, lowering blood fat, anti-inflammatory, antibacterial and anti-oxidation. Therefore, the development of Enteromorpha into functional food has a good prospect. The polysaccharides extracted from Enteromorpha enteromorpha are mainly water-soluble sulfated polysaccharides with large molecular weight and relatively low biological activity. I...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/00
Inventor 周涛许杰
Owner ZHEJIANG GONGSHANG UNIVERSITY
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