Kit for nucleic acid combined detection of influenza virus A, influenza virus B and respiratory syncytial virus

A technology of influenza B virus and influenza A virus, which is applied in the field of joint detection kits for influenza A, influenza B virus and respiratory syncytial virus nucleic acid, can solve the problem of losing the ability to be re-amplified, and there is no respiratory syncytial virus Combined detection of fluorescent PCR detection kits, false positives and other issues, to achieve the effects of short amplification time, prevention of multiple operation contamination, and simple steps
CN105400907AInactive Publication Date: 2016-03-16SHANGHAI XINGYAO MED TECH DEV CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SHANGHAI XINGYAO MED TECH DEV CO LTD
Publication Date
2016-03-16
Estimated Expiration
Not applicable · inactive patent
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Abstract

The invention provides a kit for nucleic acid combined detection of the influenza virus A, the influenza virus B and the respiratory syncytial virus. The kit comprises RT-PCR reaction liquid, an RT-PCR enzyme mixture, a respiratory virus primer probe, internal reference, negative contrast, clinical positive contrast and strong positive contrast. The one-step RT-PCR reaction can be directly conducted on the well-extracted respiratory virus nucleic acid, the influenza virus A, the influenza virus B and the respiratory syncytial virus in a sample can be detected in a classified and qualitative mode, the gene of the internal reference serves as the internal contrast, and contamination is prevented through UNG enzymes. The kit is simple in one-step amplification method, short in procedure, easy and convenient to operate, capable of preventing contamination, high in detection result specificity, high in sensitivity, clear in result, high in credibility, and capable of being used for qualitatively authenticating and detecting the influenza virus A, the influenza virus B and the respiratory syncytial virus in a human nose pharynx-mop sample.
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Description

Technical field

[0001] The present invention is a biotechnology field, involving a biological testing technology, especially involving a combination of type A, B virus and respiratory tract hypotenocyte virus nucleic acid. Background technique

[0002] VirusesAssociateDWithRespiratoryinfections refers to the general term that mainly uses the respiratory tract as an invasion of the portal.90-95%of clinical acute respiratory infections are caused by this group of poison.The respiratory virus is mostly a single -chain RNA (SSRNA) virus. It generally has envelope and thorns. It is mainly transmitted through the respiratory tract. It is positioned as the respiratory tract. It can be infected repeatedly and high population.Among them, Influenzavirus and respiratory tract sympathetic virus (RSV) are clinically and important respiratory viruses.

[0003] The form of respiratory virus virus is spherical, the genome is a linear, regardless of the section of the single -chain RNA (SSRNA),...

Claims

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