Biological feed additive rich in bioactive peptides and probiotics as well as preparation method of additive

A technology of biological feed and beneficial bacteria, which is applied in the field of biological enzymolysis and fermentation, can solve the problems of low absorption and utilization rate, and achieve the effect of high content and high active peptide content

Inactive Publication Date: 2016-03-23
上海邦成生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Cottonseed meal, rapeseed meal, soybean meal, fish meal, wheat bran, corn, rice bran and other feed materials are rich in nutrients such as crude protein, crude fiber, crude fat, crude ash, etc., and the digestion and absorption utilization rate of direct feeding animals is low

Method used

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Examples

Experimental program
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Effect test

preparation example Construction

[0029] The invention provides a preparation method of a biological feed additive rich in active peptides and beneficial bacteria, comprising the following steps:

[0030] Step 1, preparing beneficial bacteria seed liquid, adopting the method of mixing a single strain after fermentation or mixing and fermenting multiple strains at the same time, and carrying out aerobic or anaerobic fermentation at 20-40°C for 8-48 hours;

[0031] Step 2, prepare a nutrient solution, fully mix the nutrient components, add water to fully dissolve, and sterilize at 80-121°C for 10-30 minutes;

[0032] Step 3, solid-state fermentation, the beneficial bacteria seed liquid prepared in step 1, the nutrient solution prepared in step 2 together with the enzyme preparation are added to the solid-state fermentation raw materials and fully stirred and mixed. Under the ambient temperature of 5-40°C, Solid state fermentation for 12-96 hours;

[0033] Step 4, post-processing, drying the solid-state fermente...

Embodiment 1

[0036] The present embodiment is prepared through the following specific steps:

[0037] (1) Preparation of seed solution. Erlenmeyer flask medium: glucose 2.0%, bovine bone peptone 1.0%, beef extract 1.0%, yeast extract powder 0.5%, anhydrous sodium acetate 0.5%, potassium dihydrogen phosphate 0.2%, diammonium hydrogen citrate 0.2%, heptahydrate Magnesium sulfate 0.058%, manganese sulfate tetrahydrate 0.025%, Tween-800.1%. Seeds and fermentation medium: corn flour 3.0%, soybean flour 2.0%, tomato powder 0.5%, fish peptone 0.8%, yeast extract powder 0.5%, glucose 0.5%, potassium dihydrogen phosphate 0.2%, magnesium sulfate heptahydrate 0.058% , Tween -800.1%. Adjust the pH before sterilization to 6.8, sterilize at 118°C for 20 minutes, cool to 37°C and inoculate and cultivate Lactobacillus plantarum. Put the triangular flask at 37°C for static culture for 16 hours to obtain the seed liquid of the triangular flask; the pressure of the seed tank and fermentation tank is 0.01-...

Embodiment 2

[0045] The present embodiment is prepared through the following specific steps:

[0046] (1) Preparation of seed solution. Erlenmeyer flask medium: hydrolyzed milk protein 2.0%, soybean peptone 1.2%, yeast extract 0.5%, sucrose 3.0%, sodium chloride 0.5%, potassium dihydrogen phosphate 0.2%, potassium hydrogen phosphate 0.2%, magnesium sulfate heptahydrate 0.058 %, Tween-800.1%. Seed and fermentation medium: corn flour 3.0%, glucose 0.5%, tryptone 0.8%, yeast extract powder 0.5%, potassium dihydrogen phosphate 0.2%, magnesium sulfate heptahydrate 0.058%, Tween-800.1%. Adjust the pH before sterilization to 5.8, sterilize at 115°C for 30 minutes, cool to 30°C and inoculate Lactobacillus fermentum and Saccharomyces cerevisiae. Put the triangular flask at 30°C, 80rpm / min for 18 hours, and obtain the seed liquid of the triangular flask; the pressure of the seed tank and fermentation tank is 0.01-0.04MPa, and the stirring speed is 100rpm / min; the seed tank is cultivated at 30°C fo...

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PUM

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Abstract

The invention discloses a biological feed additive rich in bioactive peptides and probiotics as well as a preparation method of the additive. The biological feed additive prepared with the method has the peptide content higher than 20%, the active probiotic content up to 1.0*10<9> cfu/g and contains fermentation metabolites such as bacteriocin and the like; meanwhile, the additive has the advantages that the additive is purely natural, non-toxic, harmless and free of pollution and residues.

Description

technical field [0001] The invention belongs to the technical field of biological enzymolysis and fermentation, and in particular relates to a biological feed additive rich in active peptides and beneficial bacteria and a preparation method thereof. Background technique [0002] Cottonseed meal, rapeseed meal, soybean meal, fish meal, wheat bran, corn, rice bran, etc. are commonly used feed industry raw materials. Cottonseed meal is mainly made of cottonseed, which is obtained by pre-pressing leaching or direct leaching to remove oil. Cottonseed meal contains about 42.5% crude protein, about 10.1% crude fiber, about 0.7% crude fat, and about 6.5% crude ash. Rapeseed meal is a by-product of oil extraction from rapeseed. It contains about 38.6% crude protein, about 11.8% crude fiber, about 1.4% crude fat, and about 7.3% crude ash. Soybean meal is a by-product obtained from soybeans after soybean oil is extracted. It contains about 43.0% crude protein, about 5.1% crude fibe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/18A23K20/147C12N1/20C12N1/16C12N1/14
CPCC12N1/20C12N1/14C12N1/16
Inventor 陆克文宋士良郑云峰高潮
Owner 上海邦成生物工程有限公司
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