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Selenium-rich rhodotorulamucilaginosa FXY-7 strain, culture method thereof and application thereof to shrimp feed additive

A technology of Rhodotorula japonicus and yeast strains, applied in the field of microorganisms, to achieve the effects of enhancing immunity, saving additives, and increasing added value

Active Publication Date: 2016-03-23
HUBEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the application of high-yielding selenium-enriched rhodotorula in crayfish breeding feed has not yet been reported.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Embodiment 1: a kind of selenium-enriched gum rhodotorula FXY-7, its obtaining method is as follows:

[0014] Dilute the pickle juice sample and spread it on the plate of YEPD medium, cultivate it at 28°C, observe the growth state of the colony at regular intervals, and pick a single colony and transfer it to the YEPD slant for storage. After preliminary screening, the following yeast strains were screened: FXY-1, FXY-3, FXY-7, FXY-5, FXY-7, FXY-10, FXY-11, FXY-19, FXY-21, FXY-23, FXY-24, FXY-25, FXY-38, FXY-72, FXY-73, FXY-74, FXY-76, FXY-77, FXY-79, FXY-50, FXY-53, FXY-55, FXY- 56.

[0015] containing Na 2 SeO 3 (Final concentration is 0.5g / L) YEPD plates are coated to carry out the screening of selenium-enriched yeasts, then the yeasts obtained by the preliminary screening are cultivated with YEPD medium, and each strain is divided into blank group and experimental group for cultivation , adding Na to the experimental group 2 SeO 3 (The final concentration is 0...

Embodiment 2

[0016] Example 2: 18SITSrRNA gene sequence analysis of selenium-enriched rhodotorula FXY-7

[0017] 1. Extraction of chromosomal DNA (small amount):

[0018] (1) Take 1.5mL bacterial liquid (cultivated for 24h) in a 1.5mL Eppendorf tube, centrifuge at 12000r / min for 5min; (2) Discard the supernatant, resuspend the pellet in 900μL phosphate buffer (PBS), and centrifuge at 12000r / min for 5min at 4°C; (3) Discard the supernatant, add 300TENS and 200μL 10mg / mL lysozyme, blow and mix, incubate at 37°C for 1h, and mix by inverting every 15min; (4) Add 600TENS lysate (200mmol / LNaCl, 100mmol / LTril-HClpH8.0, 2.0%SDS, 50mmol / LEDTA, 0.5%TritonX-100) and 20mg / mL proteinase K10μL, invert and mix, incubate at 55℃ for 1h, and invert and mix once every 15min; (5) 4 Centrifuge at 12000r / min for 5min and take the supernatant; (6) Add an equal volume of P:C:I (25:24:1) to the supernatant and mix well, then centrifuge at 12000r / min for 10min at 4℃; (7) Repeat steps 6; (8) Transfer the supernat...

Embodiment 3

[0037] Example 3: BioMérieux VITEK2 automatic identification and analysis of selenium-enriched rhodotorula FXY-7

[0038] Pick the bacterial lawn of the strain to be tested and inoculate it on the YEPD plate, and cultivate it to the logarithmic growth phase at 30°C. Dip the Vitek liquid in the kit with a sterilized cotton swab, scrape off the bacterial lawn on the plate, then dissolve it in 1.8ml Vitek liquid, shake and mix well; use a turbidimeter to adjust the turbidity to the specified range (2MacF); then pipette Add the bacterial suspension to the Vitek identification plate with a gun, and the bacterial solution is ready to fill the identification hole; then take out the identification plate after incubating at 30°C for 24-48 hours, and use the BioMérieux VITEK2 automatic identification analyzer to read and analyze the data of the identification plate. The results are shown in Table 2. The physiological and biochemical results are the same as the typical physiological and...

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PUM

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Abstract

The invention discloses a screening and preparing method of selenium-rich rhodotorulamucilaginosa FXY-7 and application of selenium-rich rhodotorulamucilaginosa FXY-7 as a shrimp feed additive and belongs to the technical field of microorganisms. Rhodotorulamucilaginosa high in selenium-rich capacity is screened from pickles, then the selenium-rich rhodotorulamucilaginosa FXY-7 is obtained through a physical mutagenesis (Co60 mutagenesis) method, the preservation number of the selenium-rich rhodotorulamucilaginosa FXY-7 is CCTCC NO: M2015687, the strain can be rich in selenium in the growing and propagating process, and therefore supplement of organic selenium is achieved in feed, the organic selenium content in shrimp products is increased, the additional value of the shrimp products is increased, the adding amount of organic selenium in feed is reduced, and the feed production cost is effectively reduced.

Description

technical field [0001] The invention belongs to the technical field of microbes, in particular, the invention relates to a selenium-enriched Rhodotorulamucilaginous FXY-7, a cultivation method thereof and an application as a crayfish feed additive. Background technique [0002] Selenium (Se) is an essential trace element for animals and plays an important role in maintaining animal health, but excessive selenium will cause toxicity to the body, so research on the optimal amount of selenium in animal feed has been extensively carried out. During the paddy field model culture of crayfish, some pathogenic bacteria, especially intestinal conditional pathogens, can multiply rapidly and cause diseases. At present, the main method is to control and reduce stress by adding antibiotics in the feed. Antibiotic supplementation can inhibit the growth and reproduction of pathogenic bacteria in shrimp intestinal tract and facilitate the growth of beneficial bacteria, thereby maintaining ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16A23K10/16A23K50/80C12R1/645
Inventor 陈雄俞灿张毅代俊唐景峰周梦舟王志李欣朱莹
Owner HUBEI UNIV OF TECH
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