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A kind of halophilic desulfurization bacterial strain and its application

A bacterial strain and hydrogen sulfide technology, applied in the field of microorganisms, can solve the problems of poor stability of the biological desulfurization system, low osmotic pressure resistance, and influence on industrial applications, so as to reduce the operating cost of biological desulfurization, high osmotic pressure resistance, and less waste water The effect of discharge and consumption of lye

Active Publication Date: 2020-05-01
蒋建平 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to maintain a low osmotic pressure environment suitable for the growth of desulfurization bacteria, a large amount of high-salt washing liquid must be discharged, and a large amount of low-salt lye must be added at the same time, which will cause problems such as poor stability of the biological desulfurization system and high operating costs. affect industrial applications
The biological desulfurization strains reported in a large number of studies are limited to the laboratory research stage due to their low osmotic pressure resistance ability, and have not been applied in industrial applications.

Method used

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  • A kind of halophilic desulfurization bacterial strain and its application
  • A kind of halophilic desulfurization bacterial strain and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Experiment on halophilicity of desulfurization strain NPE-1

[0047] 1. Feed CO into NaOH solutions with different concentrations of 2%, 3%, 4%, 5%, 6%, and 7% respectively 2 , so that the pH of each NaOH solution is between 8.5-9.0, and about 4%, 6%, 8%, 10%, 12%, 14% Na 2 CO 3 and NaHCO 3 mixed solution.

[0048] 2. Add NaHS solution with a pH of 8.5-9.0 to different concentrations of NaHS 2 CO 3 and NaHCO 3 In the mixed solution, the final concentration of NaHS was kept at about 1%.

[0049] 3. To different Na 2 CO 3 and NaHCO 3 Inject the same amount of NPE-1 strains into the mixed solution of the same concentration, and place it in a shaker at 37°C for shaking culture.

[0050] 4. Observe the growth of NPE-1 strain at each concentration every 8 hours.

[0051] After testing, it was found that NPE-1 in 14% Na 2 CO 3 and NaHCO 3 In the mixed solution, the growth is slow, the number of bacteria does not increase, and the Na content below 10% 2 CO 3 and...

Embodiment 2

[0053] Desulfurization Effect Test of Desulfurization Bacteria Strain NPE-1

[0054] 1. Air source composition: H 2 S concentration 0.4%, CO 2 Concentration 45%, N 2 Concentration 54%;

[0055] 2. Test equipment: adopt figure 1 The device shown is a self-made scrubber, cylindrical, with a ground diameter of 10cm and a height of 1200cm; a self-made biological regeneration reactor, which belongs to an upflow aerobic reactor, with an effective volume of 42L, a bottom diameter of 25cm, and a total height of 100cm; elemental sulfur separator Self-made, conical, effective volume 5L, total height 60cm;

[0056] 3. Culture conditions: 37°C, pH8.5-9.0, ORP (Oxidation-Reduction Potential, oxidation-reduction potential) controlled between -320mv and -400mv;

[0057] 4. Washing liquid composition: 7%-10% Na 2 CO 3 and NaHCO 3 mixture;

[0058] After the whole system is stable, by analyzing the H in the gas entering and leaving the system 2 S concentration, the calculated NPE-1 d...

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Abstract

The invention provides a halophilic autotrophic desulfurized bacterial strain having the maximum tolerance to a 14% mixed solution of Na2CO3 and NaHCO3. The bacterial strain is named as NPE-1, and the preservation number is CCTCC NO: M 2015152. The bacterial strain can normally grow in the 7%-10% mixed solution of Na2CO3 and NaHCO3, hydrogen sulfide is converted into elemental sulfur, and the desulphurization rate can be up to 0.2-0.3 g(H2S) / (L.h). The bacterial strain has very good hydrogen sulfide load-resisting capacity and high pH resistance performance, can normally grow under the pH condition of 10.5-11 and can treat gas with the hydrogen sulfide concentration of 5000-20000 ppm. A generation cycle is short and is only 40 minutes, and the sundry bacterial resistance is strong. The invention further provides application of the halophilic desulfurized bacterial strain in biological desulphurization. The halophilic desulfurized bacterial strain can efficiently convert the hydrogen sulfide into the elemental sulfur, and also has very high halophilism, accordingly wastewater discharge and alkali liquid consumption are remarkably reduced, the operation cost of biological desulphurization is greatly reduced, the stability of a biological desulphurization system is improved, a solid foundation is laid for industrial application of a washing tower type biological desulphurization technology, and the bacterial strain is suitable for large-scale popularization and application.

Description

technical field [0001] The present invention relates to the technical field of microorganisms, more specifically, to the technical field of desulfurization microorganisms, in particular to a halophilic desulfurization bacterial strain and application thereof. Background technique [0002] With the rapid development of industrialization, human pollution to the environment is increasing. Biogas, natural gas, landfill gas and other clean gas fuels are popular among people. Biogas, natural gas, landfill gas and other gases contain a large amount of hydrogen sulfide and other sulfur compounds before they are purified. If they are directly used as fuel for combustion or power generation, they will seriously corrode equipment. Therefore, before use, the sulfur compounds in the gas need to be removed. [0003] Traditional dry desulfurization and wet desulfurization processes have been gradually replaced by biological desulfurization processes due to problems such as high desulfuri...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C10L3/10C12R1/01
CPCY02E50/30
Inventor 蒋建平张海林
Owner 蒋建平
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