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Nano particle composition and application thereof in resisting tumor angiogenesis

A nanoparticle and composition technology, applied in the field of nanoparticle composition and its application in anti-tumor angiogenesis, can solve the problems of increasing the cost of treatment and affecting the effect of treatment, etc., to prolong life or survival time, prevent Metastasis or recurrence and the effect of reducing mortality

Inactive Publication Date: 2016-04-06
XUZHOU MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the gene therapy programs of these viral vectors still have problems such as biosafety caused by genome integration and the body's immune response to viral vectors.
However, in order to achieve the therapeutic effect of polypeptide drugs, continuous high-dose repeated administration is required, which will seriously affect the therapeutic effect and increase the cost of treatment.

Method used

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  • Nano particle composition and application thereof in resisting tumor angiogenesis
  • Nano particle composition and application thereof in resisting tumor angiogenesis
  • Nano particle composition and application thereof in resisting tumor angiogenesis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Preparation of PEI-CyD-PEG-FA

[0028] A previous patent synthesized a polycationic carrier (named H1) that uses cyclodextrin (CyD) as a cross-linked backbone, connects low-molecular-weight polyethyleneimine (PEI), and couples a small-molecule drug folic acid with tumor targeting specificity. ). H1 has been proved to be able to encapsulate and concentrate the plasmid expressing the target gene into a nanoparticle with a size of about 100 nanometers in a liquid environment. In a variety of tumor cell lines, this nanoparticle showed low toxicity, high efficiency and target-specific gene delivery ability. Treatment of cancer, particularly malignant melanoma, using H1-transduced nanoparticles expressing anti-tumor cytokines.

[0029] Polyethylene glycol (PEG) is a hydrophilic polymer, which can improve the hydrophilicity of the surface of colloidal particles and the stability of ionic solutions, thereby reducing the complement conditioning and the removal of the reticu...

Embodiment

[0048] Example: 48 Balb / c mice According to the method described in the preliminary experiment, an orthotopic liver cancer mouse experimental animal model inoculated with ML-1 cells was established. Seven days after the operation, the mice were randomly divided into 5 groups, 12 mice in each group, and were given intraperitoneal injection of PCPFA / pLuc (DNA 100ug) and PCPFA / pHK1Endo (DNA 100ug) respectively. One month after treatment, 4 mice were randomly selected from each group to extract tumor tissue, weighed and collected for subsequent mechanism studies; the remaining mice continued to be observed for survival studies.

[0049] The results showed that: 30 days after treatment, the median weight of tumor tissue in the PCPFA / pLuc treatment group was 520 mg; in the PCPFA / pHK1Endo treatment group, the median weight of tumor tissue was 100 mg. This indicated that PCPFA / pHGFK1 treatment significantly inhibited the growth of liver orthotopic inoculated tumors. attached Figure...

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Abstract

The invention relates to a nano particle composition and an anti-tumor application thereof. The nano particle composition belongs to nano particles formed by self-assembly of a polymer carrier and plasmids for expressing therapeutic genes, wherein the polymer carrier is a cyclodextrin-polymine-polyethylene glycol-folic acid quaternary assembled polycation carrier, and the plasmids for expressing the therapeutic genes are eukaryotic expression plasmids containing a tricyclic fragment protein DNA sequence for encoding hepatocyte growth factors and a protein DNA sequence for encoding endostatin. Compared with the prior art, the nano particle composition has the characteristics of high efficiency and tumor targeting, and can form nano particles through self-assembly via static interaction in the water solution; the grain diameters of the nano particles and the stability of the nano particles in a saline solution play an important role in the functions of the nano particles; through verification, the nano particle composition can prevent the metastasis or relapse of cancer, alleviate, relieve, control, improve or treat primary cancer or metastatic cancer or relevant symptoms thereof, prolong the life or survival time of a cancer patient, and reduce the death rate.

Description

technical field [0001] The invention belongs to the technical field of medicine preparation, and specifically relates to a nanoparticle composition and its application in anti-tumor angiogenesis. Background technique [0002] Malignant tumor (cancer) is a major human disease, and it is of great significance to strengthen the research and development of new anti-tumor drugs. There are more than 22 million patients with malignant tumors in the world, 10.1 million new patients are diagnosed every year, and more than 6.2 million patients die of cancer every year. There are at least 3 to 4 million malignant tumor patients in my country, with more than 1.6 million newly diagnosed patients and more than 1.3 million annual deaths. With the rapid development of my country's economy and society, the extension of average life expectancy, and the changes in dietary structure and environmental factors, the incidence and mortality of tumors have shown a rapid upward trend, and the spectr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K48/00A61K9/51A61K47/40A61P35/00
CPCA61K38/1833A61K9/5161A61K38/014A61K48/0041
Inventor 姚宏郑骏年孔祥复
Owner XUZHOU MEDICAL COLLEGE