Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of preparation method of high-purity allicin

A technology of allicin and flow rate, applied in the field of preparation of high-purity allicin, can solve the problems of complicated operation of natural sources, high cost of extraction and preparation process, low purity, etc., achieve unique pharmacological activity, simplify operation process, improve recovery rate and The effect of purity

Active Publication Date: 2018-03-23
青岛博恩高科生物技术有限公司
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the current patents on the preparation of high-purity allicin, the operations of natural sources are relatively cumbersome, and the cost of extraction and preparation processes is relatively high; and the purity is not high

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The preparation method of the high-purity allicin described in the present embodiment comprises the following steps:

[0034] (1) Weigh 400mg of 2% allicin extract (commercially available, the previous ratio represents the mass content of allicin), dissolve it in 5mL of cold water, shake vigorously for 30s, let it stand for 10min, centrifuge at 3000rpm for 5min, filter through 0.22μm water system film to obtain an aqueous solution containing allicin;

[0035] (2) Select BAKERBOND spe-1g C-18 small column, activate the column with 10mL methanol and 10mL double distilled water, the flow rate is 1mL / min;

[0036] (3) Load 1.5 mL of the aqueous solution containing allicin, rinse the extraction column with 7.5 mL of 30% methanol aqueous solution at a flow rate of 1 mL / min;

[0037] (4) Elute the extraction column with 2 mL of methanol aqueous solution with a volume ratio of 90%, the flow rate is 1 mL / min, and a 10 mL graduated test tube is connected to the eluent;

[0038]...

Embodiment 2

[0048] The preparation method of the high-purity allicin described in the present embodiment comprises the following steps:

[0049] (1) Weigh 400 mg of 2% allicin extract, dissolve in 5 mL of cold water, shake vigorously for 30 s, let stand for 10 min, centrifuge at 3000 rpm for 5 min, pass through a 0.22 μm water filter to obtain an aqueous solution containing allicin;

[0050] (2) Select BAKERBOND spe-1g C-18 small column, activate the column with 10mL methanol and 10mL double distilled water, the flow rate is 1mL / min;

[0051] (3) Load 1 mL of the aqueous solution containing allicin, rinse the extraction column with 7.2 mL of methanol aqueous solution with a volume ratio of 30%, and the flow rate is 1 mL / min;

[0052] (4) Elute the extraction column with 2 mL of methanol aqueous solution with a volume ratio of 80%, the flow rate is 1 mL / min, and a 10 mL graduated test tube is connected to the eluent;

[0053] (5) Detect the purity and the concentration of allicin in the e...

Embodiment 3

[0056] The preparation method of the high-purity allicin described in the present embodiment comprises the following steps:

[0057] (1) Weigh 500 mg of 2% allicin extract, dissolve in 5 mL of cold water, shake vigorously for 30 s, let stand for 10 min, centrifuge at 3000 rpm for 5 min, pass through a 0.22 μm water filter to obtain an aqueous solution containing allicin;

[0058] (2) Select BAKERBOND spe-1g C-18 small column, activate the column with 15mL methanol and 15mL double distilled water, the flow rate is 1mL / min;

[0059] (3) Load 2.0mL of the aqueous solution containing allicin, rinse the extraction column with 7.5mL of the above 30% methanol solution, and the flow rate is 1mL / min, and drain the column (the specific steps are to obtain the flow rate by aspirating the column with a vacuum pump) , pumping out is to drain the solvent in the column to the end);

[0060] (4) Use 2.5mL of the above 90% methanol solution to elute the extraction column, the flow rate is 1mL...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
wavelengthaaaaaaaaaa
Login to View More

Abstract

The invention provides a preparing method of high purity allicin. The method comprises the specific steps: dissolving an allicin extract with cold water, standing, centrifuging and passing through a film; then purifying and preparing with a solid phase extraction method, and performing purity identification and concentration determination with an HPLC method. The specific solid phase extraction method is adopted to prepare the high purity allicin and further selects and determines a proper eluting agent in solid phase extraction to replace the allicin to an organic solvent from a water phase solution, thus improving the stability of the allicin; the invention indirectly concentrates a final product, thus improving the recovery rate and purity of the allicin. By a technical solution of the invention, a preparing operation process of the allicin is greatly simplified, and the process cost is reduced; the prepared allicin is high in purity and can be eluted with high concentration. The method can further prepare a standard product or an effective component of a medicine preparation.

Description

technical field [0001] The invention relates to a preparation method of active ingredients in garlic extract, in particular to a preparation method of high-purity allicin. Background technique [0002] Allicin (Allicin) is an organosulfur compound extracted from the bulb (garlic head) of Allium Sativum L., which is also present in onions and other Allium family plants. Allicin is a light yellow oily liquid, very unstable in nature, unstable to heat, acid, and alkali, and easily decomposed in polar solvents (such as water). Allicin has a strong antibacterial and bactericidal effect, and has an inhibitory effect on Escherichia coli, Salmonella, Staphylococcus aureus, Shigella, Salmonella typhi and other pathogenic bacteria; it can also lower blood lipids, and has a good effect on lowering cholesterol. Allicin is mostly used in food additives and biomedicine, which can increase the flavor of food and be used for the prevention and treatment of some diseases. [0003] At prese...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07C381/00
CPCC07C381/00
Inventor 公丕贤
Owner 青岛博恩高科生物技术有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com