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Phenolic acid degrading bacterium, biological organic fertilizer and application of phenolic acid degrading bacterium

An organic fertilizer and microbial technology, applied in the field of microorganisms, can solve the problems of slow growth, poor quality, and yield decline of the next apple tree, and achieve the effects of enriching nutrition, promoting plant growth, and improving soil texture

Active Publication Date: 2016-04-06
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing studies have shown that the continuous cropping obstacle of fruit trees has a great correlation with the accumulation concentration of phloridizin in the rhizosphere of fruit trees. The accumulation of high concentrations of phloridizin will cause slow growth, decreased yield, and poor quality of the next apple tree.

Method used

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  • Phenolic acid degrading bacterium, biological organic fertilizer and application of phenolic acid degrading bacterium
  • Phenolic acid degrading bacterium, biological organic fertilizer and application of phenolic acid degrading bacterium
  • Phenolic acid degrading bacterium, biological organic fertilizer and application of phenolic acid degrading bacterium

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Isolation, Identification and Preservation of Example 1 Bacterial Strain

[0034] 1. Isolation of Novosphingobium Subarcticum AMCC100069 strain

[0035] Sampling time: September 2011

[0036] Sampling location: Shanhoubo Village, Guanli Town, Qixia City, Shandong Province

[0037] 74 strains with the ability to degrade apple phlorizin and other phenolic acids were isolated and screened from soil samples collected from the rhizosphere of apple continuous cropping gardens around the Bohai Sea, and one of them was named AMCC100069.

[0038] 2. Identification of Novosphingobium Subarcticum AMCC100069 strain

[0039] For the colony morphology and microscopic morphology of AMCC100069 strain, see figure 1 .

[0040] The colony is small, yellow and shiny, the surface is smooth and moist, the center of the colony is semicircular, and it does not produce pigment. Gram-negative bacteria G- , Indole test, V-P test, nitrate reduction and gelatin liquefaction were negative, methy...

Embodiment 2

[0044] Embodiment 2, new sphingosine bacillus (Novosphingobium Subarcticum) AMCC100069 bacterial strain degradability indoor determination

[0045] 1. Laboratory determination of the degradation ability of the new sphingobacterium (Novosphingobium Subarcticum) AMCC100069 strain

[0046] Inoculate the new sphingobacterium (Novosphingobium Subarcticum) AMCC100069 strain into the activation medium for activation culture for 24 hours, the temperature is 28-30°C, and the stirring speed is 180-200r / min;

[0047] The components per liter of activation medium are as follows:

[0048] Phlorizin 0.96g, MgSO 4 ·7H 2 O0.2g, NH 4 h 2 PO 4 1g, KCl0.2g, dilute water to 1000mL, pH7.0, sterilize at 121°C for 20min;

[0049] Neosphingobium Subarcticum (Novosphingobium Subarcticum) AMCC100069 after activation was inoculated into 2mmol / L phlorizin liquid culture medium (substrate composition and quality: phlorizin 0.96g, MgSO 4 .7H 2 O0.2g, NH 4 h 2 PO 4 1g, KCl0.2g, distilled water to...

Embodiment 3

[0058] Embodiment 3, preparation and pot test of microbial organic fertilizer

[0059] 1. Preparation of microbial organic fertilizer

[0060] 1. Inoculate the new sphingobacterium (Novosphingobium Subarcticum) AMCC100069 strain into the activation medium for activation culture for 24 hours, the temperature is 28-30°C, and the stirring speed is 180-200r / min;

[0061] The components per liter of activation medium are as follows:

[0062] Phlorizin 0.96g, MgSO 4 ·7H 2 O0.2g, NH 4 h 2 PO 4 1g, KCl0.2g, dilute water to 1000mL, pH7.0, sterilize at 121°C for 20min;

[0063] The activated Neosphingobium Subarcticum (Novosphingobium Subarcticum) AMCC100069 was inoculated into the fermentation medium at an inoculum size of 2% (mass percentage), the fermentation temperature was 28-30°C, and the stirring speed was 180-220r / min, so that the fermentation broth The content of bacteria in the medium is not less than 1.0×10 10 cfu / g;

[0064] The composition of the fermentation mediu...

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Abstract

The invention relates to a phenolic acid degrading bacterium, a biological organic fertilizer and application of the phenolic acid degrading bacterium. Novosphingobium subarcticum AMCC100069 is collected in the China General Microbiological Culture Collection Center (CGMCC) (address: No. 3, Building #1, West Beichen Road, Chaoyang District, Beijing) on July 31, 2015 with a culture collection number CGMCC No.11163. The novosphingobium subarcticum AMCC100069 has a remarkable degrading effect on phenolic acids such as phlorizin causing apple successive cropping obstacles, has a remarkable effect in facilitating growth of fruit trees, and can effectively relieve the problem of apple successive cropping obstacles in production.

Description

technical field [0001] The invention relates to a phenolic acid substance-degrading bacterium and its bio-organic fertilizer and its application, belonging to the technical field of microbes. Background technique [0002] At present, my country's apple production has jumped to the first place in the world, but as the existing orchards gradually enter the replacement period of old trees and new ones, fruit trees are also facing serious obstacles to continuous cropping. The main manifestations are weak growth of newly planted young plants (seedlings), dwarfing of plants, shortened internodes, small leaves, poor root development, susceptibility to rot, and low stress resistance of the tree. [0003] The allelochemical substances produced by plants through the volatilization of stems and leaves, leaching, root secretion and decomposition of plant residues are the core issues affecting the rhizosphere environment. Studies have found that phenolic acids in root exudates are curren...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F11/08C12R1/01
CPCC05F11/08C12N1/205C12R2001/01
Inventor 周波刘淼王芳张铭铄毛志泉束怀瑞
Owner SHANDONG AGRICULTURAL UNIVERSITY
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