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In-vitro rapid propagation method for nepenthes

A pitcher plant, in vitro technology, applied in the field of plant tissue culture, can solve the problems of slow germination, slow rooting, difficult to survive, etc., and achieves the effects of inducing young shoots quickly, increasing effective seedlings, and reducing losses

Inactive Publication Date: 2016-04-27
FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the existing nepenthes propagation by seeds, the germination speed is slow and the germination rate is low; the cutting propagation is slow, the rooting speed is slow, and it is not easy to survive. The invention provides a rapid in vitro propagation method for nepenthes

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) Selection and treatment of explants

[0027] Select a robust Nepenthes plant, use terminal buds or stem segments with side buds as explants, spray the whole plant with 75% chlorothalonil wettable powder 800 times liquid in 4-5 days before cutting the explants, And cover the part of the plant pre-selected as explants with a colorless transparent bag; cut the explants with a length of 3-5 cm and rinse them with running water, remove the surface dirt, and disinfect the surface with alcohol with a volume fraction of 75% for 30 seconds then first put into the mercuric chloride solution with a mass concentration of 0.1% for disinfection for 15min, then put into the mixed solution for disinfection for 20min, then rinse with sterile water for 3 to 4 times; % sodium hypochlorite solution was added dropwise with 2 drops of Tween-20;

[0028] (2) Induction culture

[0029] Inoculate the explants treated in step (1) into a culture medium with a pH of 5.8 to 6.0, in which MS i...

Embodiment 2

[0038] Embodiment 2 is the same as Embodiment 1 except that the following steps are different.

[0039] (2) Induction culture

[0040]Inoculate the explants treated in step (1) into a culture medium with a pH of 5.8 to 6.0, in which MS improved culture fluid + 6-BA2.0mg / L + NAA0.1mg / L + sucrose 30000mg / L + agar 6250mg / L were inoculated, Under the culture conditions that the light intensity is 1500~2000lx, the temperature is 25℃±2℃, and the light time is 12h / d, after 7 days of culture, transfer to 1 / 2MS+6-BA1.0mg / L+NAA0.1mg / L+activated charcoal 0.05g / L+sucrose 30000mg / L+agar 6250mg / L, in the culture medium that pH is 5.8~6.0, cultivate under the same culture conditions as described in the second step (2) of this embodiment until the explants germinate and differentiate Young shoots with a height of 2.5 to 3.0 cm; the MS improved culture medium is the same as the MS improved culture medium described in step (2) of Example 1.

[0041] (3) Subculture

[0042] Cut the 2.5-3.0cm...

Embodiment 3

[0046] Embodiment 3 is the same as Embodiment 1 except that the following steps are different.

[0047] (3) Subculture

[0048] Cut the 2.5-3.0cm-high young shoots differentiated in step (2) into individual plants, and cut off the terminal buds, then transfer to 1 / 2MS+6-BA2.5mg / L+NAA0.2mg / L+activated carbon 0.05 g / L+sucrose 30000mg / L+agar 6250mg / L, pH is 5.8~6.0 in the culture medium, under the same culture condition as embodiment 1 step (2), cultivate 35d transfer to 1 / 2MS+6-BA1. 5mg / L+NAA0.1mg / L+coconut juice 100ml / L+sucrose 30000mg / L+agar 6250mg / L, in the culture medium that pH is 5.8~6.0, cultivate under the same culture condition as embodiment 1 step (2) for 30 days .

[0049] (5) Transplanting and management of tissue culture seedlings

[0050] The nutrient pot seedlings of Nepenthes were obtained after culturing for 40 days.

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Abstract

The invention discloses an in-vitro rapid propagation method for nepenthes. According to the method, a terminal bud or a stem with a lateral bud is taken as an explant, the whole plant is sprayed with chlorothalonil 4-5 days before the explant is sheared off, and the pre-selected explant is wrapped with a transparent bag; two culture mediums are used for alternate culture during induction culture and subculture, and MS improvement is performed. Nutritional bowl seedlings of nepenthes are obtained quickly through rooting culture as well as tissue culture seedling transplanting and management. The contamination rate of the explant is greatly reduced through pre-harvest treatment of the explant, an MS culture medium is improved, the propagation effect is prominent, and a good foundation is laid for quick culture of tissue culture seedlings of nepenthes. The terminal bud is cut off in advance, the problem that the explant only grows high but does not grow buds in the culture process is solved, a large quantity of buds are obtained in a short culture time, the two culture mediums are alternatively used in a short time, buds are quickly induced, and effective seedlings are increased; with the application of activated carbon in induction culture and subculture, browning of materials is effectively prevented.

Description

technical field [0001] The invention relates to a method for in vitro culture and rapid propagation of nepenthes, belonging to the technical field of plant tissue culture. Background technique [0002] Nepenthes, also known as piglet cages, thunder pots, etc., is a perennial herb or semi-lignified vine of the Nepenthes family Nepenthes. It is native to southeastern Asia and northern Oceania. There are about 67 species in the world, although in my country's Hainan and Guangdong's Zhanjiang, Zhuhai, Zhongshan and other parts of the distribution, but rarely used. Until after the 1990s, the fine varieties of Nepenthes introduced into China were mainly used in flower exhibitions. [0003] Nepenthes has become an eye-catching ornamental plant. The cage-shaped metamorphosis leaves that change from the leaves have a unique shape, and at the same time have insectivorous characteristics, which is extremely interesting. One of the most popular species of plants. Their sizes and color...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/005A01H4/008
Inventor 杨春梅单芹丽汪国鲜屈云慧蒋海玉阮继伟吴丽芳曹桦缑辉
Owner FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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