Method for culturing Rhodopseudomonas photosynthetic bacteria by using cyanobacterial bloom

A technology of rhodopseudomonas and cyanobacteria blooms, applied in the direction of microorganism-based methods, bacteria, chemical instruments and methods, etc., can solve the problems of consuming dissolved oxygen in water bodies and the limitation of cyanobacteria bloom resources utilization, etc., to achieve Resource utilization, short cultivation cycle, economic cost and time saving effects

Active Publication Date: 2016-05-04
NANJING INST OF GEOGRAPHY & LIMNOLOGY
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  • Summary
  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Because cyanobacteria cells in cyanobacteria blooms may release cyanotoxins after they rupture, if there is no certain environmental protection and safe treatment, cyanobacteria blooms will easily produ...

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0022] In the high temperature July, the cyanobacteria bloom (the dominant species is the genus Microcystis cyanobacteria) was taken from the breeding water body where the cyanobacteria bloom occurred, and the concentrated cyanobacteria bloom was obtained by filtering with a 100-mesh filter. At this time, the cyanobacteria bloom appeared Slurry, referred to as cyanobacteria pulp for short, is placed in a glass tank in a glass greenhouse. The total volume of the glass tank is 70L and the height is 40cm. There is no shade around the glass tank, and the volume of blue-green algae slurry reaches 65L. At this time, the total nitrogen concentration of the suspended mixture in the tank is 230mg / L, and the total phosphorus concentration is 35mg / L (the determination of total nitrogen and total phosphorus refers to the alkali in "Lake Eutrophication Investigation Standards" (Second Edition) combined digestion with potassium persulfate followed by spectrophotometric measurement). Then a...

Embodiment 2

[0025] At the end of July at high temperature, a mixture of cyanobacteria blooms and in-situ water was obtained from Taihu Lake where cyanobacteria blooms (the dominant species is Microcystis cyanophyta) occurred, and the concentrated cyanobacteria blooms were obtained by filtering with a 120-mesh filter. The cyanobacteria bloom is in the form of slurry, referred to as cyanobacteria pulp. It was transferred to a white plastic barrel with a total volume of 100 L by the lake, and the height of the barrel was 80 cm. There is no shade around the barrel. The volume of cyanobacteria slurry reaches 80L. At this time, the total nitrogen concentration of the suspended mixture in the white plastic barrel was 1005 mg / L, and the total phosphorus concentration was 169 mg / L. Then the mixture in the barrel is aerated. The aeration is done by connecting the air stone to the air pump. Two air stones are placed in the barrel (the diameter of the air stone is 2.5cm, and the height is 5cm). The...

Embodiment 3

[0028] In high temperature August, the mixture of cyanobacteria bloom (the dominant species is Microcystis cyanophyta) and in-situ water was obtained from the aquaculture water body where cyanobacteria bloom occurred, and the concentrated cyanobacteria bloom was obtained by filtering with a 120-mesh filter. At this time, the cyanobacteria bloom is in the form of slurry, which is referred to as cyanobacteria pulp for short. It is placed in a plexiglass column with a total volume of 130 L in a glass greenhouse, and the column is 100 cm high. There is no shade around the column. The volume of concentrated cyanobacteria bloom reaches 120L. At this time, the total nitrogen concentration of the mixture in the suspended state in the column was 895 mg / L, and the total phosphorus concentration was 120 mg / L. Then carry out aeration treatment on the cyanobacteria bloom in the column. The air pump is connected to the air stone for aeration. There are 3 cylindrical air stones (air stone d...

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Abstract

The invention discloses a method for culturing high-density Rhodopseudomonas photosynthetic bacteria by using cyanobacterial bloom, which comprises the following steps: acquiring cyanobacterial bloom from a cyanobacterial bloom generating water body, and concentrating properly until the total nitrogen concentration in the concentrated cyanobacterial bloom system is 230-2010 mg/L and the total phosphorus concentration is 35-350 mg/L; and putting in an open transparent container in a light environment, and continuously aerating while keeping the concentrated cyanobacterial bloom system at 20-42 DEG C to form an coexistence of an aerobic region and an anaerobic region until the high-density photosynthetic bacteria are generated. The method can be used for converting the cyanobacterial bloom into the Rhodopseudomonas photosynthetic bacteria, is capable of promoting the innocent treatment and utilization of water body nutrient substances and accelerating the conversion of the water body nutrient substances, and can be directly used for treating the cyanobacterial bloom; and the formed photosynthetic bacteria can be directly used for breeding industry or sewage purification.

Description

technical field [0001] The invention relates to a method for cultivating microorganisms in water bodies, in particular to a method for cultivating Rhodopseudomonas ( Rhodopseudomonas ) method of photosynthetic bacteria. Background technique [0002] Photosynthetic bacteria (Photosynthetic Bacteria) is the general term for prokaryotic organisms that first appeared on the earth, are ubiquitous in nature, and have primitive photosynthetic systems. One group of photoheterotrophic bacteria that contain bacteriochlorophyll and carotenoids, are capable of photosynthesis, have diverse photosynthetic inner membranes, and use sulfide or sulfate or other substances as electron donors is called purple bacteria. Purple bacteria are a large class of photosynthetic bacteria, and they have been widely used in aquaculture and sewage treatment in recent years. Because purple bacteria contain different types of carotenoids, the cell culture medium can appear purple, red, orange-brown, yellow...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01
CPCC02F3/34C12N1/20
Inventor 秦伯强高光张运林朱广伟汤祥明吴挺峰董百丽许海邓建明
Owner NANJING INST OF GEOGRAPHY & LIMNOLOGY
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