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Rhizopus microsporus C97102734 and application

A technology of Rhizopus microsporus and Dendrobium, applied in the field of preparation of dendrobium liquefied liquid, to achieve the effect of improving health care function, shortening sterilization time and improving quality of life

Active Publication Date: 2016-05-25
北京本草通汇科技中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Dendrobium polysaccharide is the main active ingredient of Dendrobium. So far, health wine produced from Dendrobium can be divided into soaked wine and distilled wine. For example, invention patents CN101451098A, CN104352050A, CN104127700A, CN103666959A, and CN103849542A are representatives of soaked wine. Soaked in wine and completely dissolved in wine, even if the polysaccharide of Dendrobium candidum is soaked in water, it can only dissolve the alkaloids and pigments in the dendrobium in the wine, and it is difficult to exert the effect of disease prevention and health preservation of Dendrobium; such as the invention patent CN103666960A is a representative of distilled wine, adding Dendrobium to rice to ferment distilled wine, but active ingredients such as Dendrobium polysaccharides cannot be completely dissolved in the wine

Method used

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  • Rhizopus microsporus C97102734 and application
  • Rhizopus microsporus C97102734 and application
  • Rhizopus microsporus C97102734 and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1 separate fermentation method prepares dendrobium liquefaction liquid

[0047] Wash 180kg of fresh dendrobium branches rich in organic selenium components, cut fresh dendrobium strips and fresh flowers into 3mm slices with a frequency-variable and variable-speed high-efficiency chopper, and then send them to a frequency-variable and variable-speed juicer, and add 1260kg of water to the juicer. After the juicer is turned on, its speed changes from slow to fast, and the speed is controlled in the range of 10 to 1000 rpm. After all the juice is pumped out, the Dendrobium juice is transported to the sterilization room with a pump and a pipeline, and the gamma irradiation machine is used at 2500 k Rad irradiation sterilization, the dendrobium juice after sterilizing is transported in the aseptic fermenter of 2 tons with aseptic pump and aseptic pipeline, and according to 9 parts of dendrobium juices plus 1 part of Rhizopus microsporus C97102734 seed liquid Ratio,...

Embodiment 2

[0049] Embodiment 2 separate enzymolysis method prepares dendrobium liquefaction liquid

[0050] Wash 180kg of fresh dendrobium branches rich in organic selenium components, cut fresh dendrobium strips and fresh flowers into 3mm slices with a frequency-variable and variable-speed high-efficiency chopper, and then send them to a frequency-variable and variable-speed juicer, and add 1260kg of water to the juicer. After the juicer is turned on, its speed changes from slow to fast, and the speed is controlled in the range of 10 to 1000 rpm. After all the juice is pumped out, the Dendrobium juice is transported to the sterilization room with a pump and a pipeline, and the gamma irradiation machine is used at 2500 k Rad irradiation sterilization, the dendrobium juice after the sterilization is transported in the aseptic fermentation tank of 2 tons with aseptic pump and aseptic pipeline, and 0.12kg probiotic neutral β-mannanase is added thereto, in Enzymolysis at 50°C-55°C and pH6-6....

Embodiment 3

[0052] Example 3 Preparation of dendrobium liquefied liquid by first enzymolysis and then fermentation

[0053] Wash 180kg of fresh dendrobium branches rich in organic selenium components, cut fresh dendrobium strips and fresh flowers into 3mm slices with a frequency-variable and variable-speed high-efficiency chopper, and then send them to a frequency-variable and variable-speed juicer, and add 1260kg of water to the juicer. After the juicer is turned on, its speed changes from slow to fast, and the speed is controlled in the range of 10 to 1000 rpm. After all the juice is pumped out, the Dendrobium juice is transported to the sterilization room with a pump and a pipeline, and the gamma irradiation machine is used at 2500 k Rad irradiation sterilization, the dendrobium juice after sterilizing is transported in the aseptic fermentation tank of 2 tons with aseptic pump and aseptic pipeline, and adds 0.08kg probiotic neutral β-mannanase thereinto, in Enzymolysis at 50°C and pH f...

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Abstract

The invention relates to rhizopus microsporus C97102734, and the preservation number is CCTCC NO.M2015706. The invention further relates to a method for preparing dendrobium nobile liquefying liquid by liquefying dendrobium nobile through the rhizopus microsporus C97102734. The preparation method comprises the steps that dendrobium nobile stems or flowers are taken to be cut up, juice extracting is performed by adding water, dendrobium nobile juice is inoculated into probiotic neutral beta-mannase for primary enzymolysis and then inoculated into the rhizopus microsporus C97102734 for fermentation after being sterilized, the liquefied juice is filtered, filtrate is taken, then the dendrobium nobile liquefying liquid is obtained, and the obtained filtrate can be used for liquor blending. According to the rhizopus microsporus C97102734 and the method, dendrobium polysaccharides insoluble in liquor are converted into dendrobium oligosaccharides soluble in the liquor through an enzymolysis mode or a fermentation mode or the mode of a combination of enzymolysis and fermentation, and therefore the technical problems that dendrobium polysaccharides are insoluble in the liquor, and qualified liquor cannot be produced through dendrobium nobile are solved; the liquor is blended with dendrobium oligosaccharides, the obtained dendrobium nobile liquor has the good health care function, deep processing of dendrobium nobile is achieved, and the additional value of dendrobium nobile is significantly increased.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to Rhizopus microsporus C97102734 and its application, and also relates to a method for preparing dendrobium liquefaction liquid by using Rhizopus microsporus C97102734. Background technique [0002] Weakness of the spleen and stomach mainly manifests as weakness in the limbs, weakness of the whole body, repeated diarrhea, loss of appetite, inability to change old age, pale complexion and other symptoms. Poor stomach and intestines affect the whole body function, mood and energy. The popularization of tools, high mental stress, often eating junk food and beverages, especially students and office workers with weak spleen and stomach, usually need food and beverages to prevent and treat weak spleen and stomach. [0003] Dendrobium officinale, Dendrobium officinale, and Dendrobium chrysalis are precious traditional Chinese medicines for both medicine and food. They are flat in ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P19/00C12G3/02C12R1/845
CPCC12G3/02C12P19/00C12N1/145C12R2001/845
Inventor 刘金龙毕文刚王安江刘欋霄
Owner 北京本草通汇科技中心
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