4 results about "DENDROBIUM NOBILE STEM" patented technology

The invention belongs to the technical field of liquid fermentation of endophytic fungi, and particularly relates to a method for producing dendrobine by co-culture of endophytic fungi and dendrobium nobile and a special strain. According to the method, fusarium endophytic fungi which are preserved in the China General Microbiological Culture Collection Center (CGMCC) and have the preservation number of CGMCC NO.3.27824 are adopted, dendrobium nobile stems or dry powder is added into an optimized liquid culture medium to serve as a homologous stimulant for co-culture, and the yield of dendrobine is remarkably increased. Experiments show that by means of the method, the yield of dendrobine can be increased by dozens of times or above and up to about 99 times, the process is stable, cost is low, and large-scale production is easy to achieve. The invention provides a new way for large-scale green production of dendrobine, and reduces the dependence on wild dendrobium resources.

The application provides a culture medium for producing dendrobium stem cell exovesicles and an application thereof, relates to the technical field of biology, and the culture medium for producing dendrobium stem cell exovesicles comprises 0.1-0.5 mg / L naphthalene acetic acid, 0.05-0.2 mg / L thidiazuron, 0.5-5 muM sodium selenate and 1-10 mg / L glutathione. The culture medium for producing dendrobium stem cell exovesicles and the method for preparing dendrobium stem cell exovesicles by using the culture medium for producing dendrobium stem cell exovesicles provided by the application have made significant progress in the yield and quality of dendrobium stem cell exovesicles. Meanwhile, the prepared dendrobium stem cell exovesicles have significant DPPH free radical scavenging capacity and improve the healing capacity of skin fibroblasts.

The invention relates to the technical field of cosmetics, in particular to a tightening and brightening composition containing a white truffle extract and application of the tightening and brightening composition. The composition comprises a white truffle fungus extract, a dendrobium devonianum stem extract, a spirulina maxima extract and a pyracantha fortuneana fruit extract. The composition obtained by compounding the raw materials can significantly inhibit the activity of elastase and collagenase, has a certain whitening effect, and is mild and non-irritating.

The invention provides a preparation method and application of superfine crushed dendrobium nobile, and belongs to the technical field of traditional Chinese medicine processing and application, the preparation method comprises the following steps: 1) airing fresh dendrobium nobile stems, cutting into segments, and drying to obtain dry stem segments; 2) pre-crushing the dried stem segments, performing first screening, and collecting undersize components to obtain fine powder; and (3) carrying out superfine grinding on the fine powder, carrying out secondary sieving, and collecting undersize components as superfine powder. According to the preparation method disclosed by the invention, the structure of the dendrobium nobile is greatly destroyed, the particle size of the powder is reduced, the cell wall breaking rate is increased, and the leaching and release of active ingredients such as polysaccharides, dendrobine, total polyphenols and total flavonoids in the dendrobium nobile are promoted; compared with conventional fine powder, the dendrobium nobile submicron powder prepared by the preparation method disclosed by the invention has the advantage that the liver protection activity of dendrobium nobile can be remarkably enhanced.