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Fat mesenchymal progenitor cell and platelet-rich blood plasma composition for treatment of hepatitis B

A technology for platelets and progenitor cells, which is used in drug combinations, medical raw materials derived from mammals, medical preparations containing active ingredients, etc.

Inactive Publication Date: 2016-06-08
CELLULAR BIOMEDICINE GRP SHANGHAI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] To sum up, there is still a lack of a method in this field that can effectively treat hepatitis B, especially the method that can completely remove hepatitis B virus and repair liver cell necrosis caused by hepatitis.

Method used

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  • Fat mesenchymal progenitor cell and platelet-rich blood plasma composition for treatment of hepatitis B
  • Fat mesenchymal progenitor cell and platelet-rich blood plasma composition for treatment of hepatitis B
  • Fat mesenchymal progenitor cell and platelet-rich blood plasma composition for treatment of hepatitis B

Examples

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preparation example Construction

[0091] In the present invention, the preparation method of adipose mesenchymal progenitor cells may include the steps of: washing adipose tissue, then digesting it with collagenase, centrifuging to separate stromal and vascular components, removing oil and collagenase, culturing primary cells, and obtaining passaged adipose tissue mesenchymal progenitor cells.

[0092] Antigen detection of adipose-derived mesenchymal progenitor cells

[0093] The adipose-derived mesenchymal progenitor cells used in the present invention have high purity and basically do not contain other types of cells or stem cells. This can be verified by detection of cell surface antigens.

[0094] Adipose mesenchymal progenitor cells have a variety of specific antigens and receptors, mainly including CD3, CD13, D29, CD34, CD45, CD49e, CD59, CD73, CD90, CD105, HLA-ABC, etc.

[0095] CD34 antigen is a highly glycosylated type I transmembrane protein, which is selectively expressed on the surface of human h...

Embodiment 1

[0116] The preparation of embodiment 1haMPC

[0117] 1. Sterile surgical instruments and consumables

[0118] (1) 5 sterile long-handled surgical forceps

[0119] (2) Sterile 100 mesh filter

[0120] (3) Sterilized 40-mesh filter

[0121] (4) 50ml centrifuge tube

[0122] (5) T175, T75 culture flask

[0123] (6) T10ml, T25ml pipettes

[0124] (7) Wide mouth tip pipette

[0125] 2. Sterile reagents:

[0126] (1) DMEM (serum-free medium), MSCSFM medium (life)

[0127] (2) Collagenase type I (preparation and use): 0.1% collagenase I Preparation method: Weigh 0.1g collagenase I powder and dissolve it in 100ml medium without adding any factors, and preheat at 37°C before use.

[0128] (3) Sodium chloride injection

[0129] (4) 0.125% Trypsin-0.01% EDTA solution

[0130] (5) 1g / L sodium citrate

[0131] Adipose-derived mesenchymal progenitor cell preparation:

[0132] 1. To receive adipose tissue, wipe the outer wall of the container containing adipose tissue with 75% ...

Embodiment 2

[0151] Example 2 Immunostaining Analysis of Adipose Stem Cell Differentiation (Adipogenic Induction Control and Oil Red O Staining Experiment)

[0152] Divide haMPCs in 1.5×10 5 The density of cells / well was inoculated in a six-well plate, and the sample cultured in normal medium was used as the negative control group of the adipogenic differentiation experiment. The specific method is: add 1 μmol / L dexamethasone, 10 μmol / L insulin, 200 μmol / L indomethacin and 0.5 mmol / L isobutyl methyl to the base medium (DMEM+10% fetal bovine serum). Xanthine was prepared into adipogenic induction medium, and the medium was changed twice a week until adipogenic staining was observed. Parallel experiments were carried out in the above control groups (n=3).

[0153] The samples of each group after adipogenic induction were stained with 0.5% Oil Red O. The specific operation method is: first wash the sample cells fully with D-hanks, and then add oil red to dilute and stain for 10-15 minutes. ...

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Abstract

The invention relates to a use of a fat mesenchymal progenitor cell and platelet-rich blood plasma composition in prevention or treatment of hepatitis, particularly, relates to a use of the fat mesenchymal progenitor cell and platelet-rich blood plasma composition in preparation of a drug composition for treatment of hepatitis B. After a required object is administered with the drug composition, indexes such as hepatitis B surface antigen, hepatitis B surface antibody, hepatitis B e antigen, hepatitis B e antibody, hepatitis B core antibody and the like can be improved, hepatitis virus DNA can be significant reduced, and ALT can be reduced so as to promote restoration of liver functions.

Description

technical field [0001] The present invention relates to the application field of fat stem cells, and specifically, the invention provides a use of fat stem cells for treating hepatitis B. Background technique [0002] Liver disease is divided into viral liver disease and non-viral hepatitis. Viral hepatitis mainly includes A, B, C, D, and E viral hepatitis, and non-viral liver disease mainly includes alcoholic liver disease, drug or toxic liver disease, metabolic abnormal liver disease, and fatty liver disease. Chronic hepatitis B has a global distribution and can lead to diseases including hepatic decompensation, cirrhosis, and hepatocellular carcinoma. Active hepatitis B virus (HBV) replication is a major driver of liver injury and disease progression. [0003] Severe hepatitis B is a severe hepatitis caused by HBV infection. Its onset is dangerous and progresses rapidly. The prognosis of most patients is poor, and large areas of liver cell necrosis can occur rapidly, le...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/35A61P1/16A61P31/20A61K35/16
Inventor 曹卫张丽戴成祥刘佳蔡松柏郑成小
Owner CELLULAR BIOMEDICINE GRP SHANGHAI
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