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A method for extracting and purifying cordycepin from Cordyceps militaris culture fluid

A culture solution and cordycepin technology, applied in chemical instruments and methods, organic chemistry, sugar derivatives, etc., can solve the problems of complex extraction process, environmental and artificial hazards, and insufficient purity of cordycepin, and achieve simple process and low production cost Low, easily mass-producible effect

Active Publication Date: 2019-05-14
CHANGSHU JINDA TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to solve the problem that the solvent used in the extraction process in the prior art is seriously harmful to the environment and artificially, and the extraction process is complicated, resulting in insufficient purity of cordycepin, a method for extracting and purifying cordycepin from Cordyceps militaris culture solution is proposed. Methods

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A method for separating and purifying cordycepin from Cordyceps militaris culture fluid, comprising the following steps:

[0026] (1) Obtain 500ml of Cordyceps militaris culture fluid, centrifuge the culture fluid, centrifuge at 7500rpm for 10min, collect the supernatant, and concentrate it to 10ml by rotary evaporation.

[0027] (2) Use the concentrated culture solution on the macroporous resin column HP-20, the column volume is 100ml, the elution solvent is water, 15% ethanol, 95% ethanol in sequence, the flow rate is 3ml / min, and the elution solvent volume is 300ml water (30ml / tube, 10 tubes), 450ml of 15% ethanol (30ml / tube, 15 tubes), 400ml of 95% ethanol (collected together). The HPLC detection of the received eluent showed that cordycepin was concentrated in the 15% ethanol eluate with a purity higher than 90%. The 15% ethanol eluate was collected and concentrated to 10ml by rotary evaporation.

[0028] (3) Put the concentrated solution of (2) in a refrigerato...

Embodiment 2

[0030] A method for isolating and purifying cordycepin from Cordyceps militaris culture fluid, comprising the following steps:

[0031] (1) Obtain 1L of Cordyceps militaris culture fluid, filter the culture fluid with filter paper, and then concentrate it to 20ml by rotary evaporation.

[0032] (2) Use the concentrated culture solution on the macroporous resin column D101, the column volume is 500ml, the elution solvent is water, 10% ethanol, 95% ethanol in sequence, the flow rate is 10ml / min, the elution solvent volume is 2L of water (200ml / bottle, 10 bottles), 10% ethanol 2.4L (200ml / bottle, 12 bottles), 95% ethanol 1L (collected together). The HPLC detection of the received elution solvent showed that cordycepin was concentrated in the 10% ethanol eluent with a purity higher than 90%. The 10% ethanol eluate was collected and concentrated to 10ml by rotary evaporation.

[0033] (3) Put the concentrated solution of (2) in a refrigerator at 4°C overnight to obtain cordycepin...

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Abstract

The invention provides a method for extracting purified cordycepin from cordyceps militaris nutrient solution. The method comprises the steps of: taking a cordyceps militaris nutrient solution as a raw material, filtering or centrifuging, and concentrating a supernatant by rotary evaporation; filling a macroporous resin column with a concentrated liquid, then eluting with water and ethanol, collecting an ethanol eluant and concentrating; and crystallizing at low temperature. According to the method, the liquid medium is used, extracting by macroporous resin and eluting by using water and an alcohol solvent are conducted; and the process has no serious contamination or waste liquid disposal. Compared to the complex extraction process of the prior art, the three-step extraction method of the invention has the advantages of low production cost, simple process and easiness to mass production. The generated cordycepin is in white needle or crystalline shape, and has high purity reaching more than 95%, which is improved significantly compared to the 90% in the prior art.

Description

technical field [0001] The invention relates to a method for extracting and purifying cordycepin, which belongs to the field of separation of effective components of medicinal fungi. Background technique [0002] Cordyceps militaris (L.) Link, also known as Cordyceps militaris and Cordyceps militaris, belongs to the genus Cordyceps ergotaceae, and is a worldwide widespread species. The metabolites of Cordyceps militaris contain a variety of active compounds, such as cordycepin, adenosine, cordyceps polysaccharide, mannitol, SOD enzyme and ergosterol, etc., which have various biological activities, including antitumor. [0003] Cordycepin is the main active ingredient of Cordyceps militaris. It is needle-shaped or leaf-shaped crystals. Its chemical name is 3’-deoxyadenosine, its molecular formula is C10H13O3N5, its molecular weight is 251.9Da, and its melting point is 225~26°C. Cordycepin is the first nucleoside antibiotic isolated from fungi. It has the maximum absorption a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H19/16C07H1/06
CPCC07H1/06C07H19/16
Inventor 杨智钧王锦锦吕爱平卞兆祥
Owner CHANGSHU JINDA TECH CO LTD
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