A method for extracting and purifying cordycepin from Cordyceps militaris culture fluid
A culture solution and cordycepin technology, applied in chemical instruments and methods, organic chemistry, sugar derivatives, etc., can solve the problems of complex extraction process, environmental and artificial hazards, and insufficient purity of cordycepin, and achieve simple process and low production cost Low, easily mass-producible effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0025] A method for separating and purifying cordycepin from Cordyceps militaris culture fluid, comprising the following steps:
[0026] (1) Obtain 500ml of Cordyceps militaris culture fluid, centrifuge the culture fluid, centrifuge at 7500rpm for 10min, collect the supernatant, and concentrate it to 10ml by rotary evaporation.
[0027] (2) Use the concentrated culture solution on the macroporous resin column HP-20, the column volume is 100ml, the elution solvent is water, 15% ethanol, 95% ethanol in sequence, the flow rate is 3ml / min, and the elution solvent volume is 300ml water (30ml / tube, 10 tubes), 450ml of 15% ethanol (30ml / tube, 15 tubes), 400ml of 95% ethanol (collected together). The HPLC detection of the received eluent showed that cordycepin was concentrated in the 15% ethanol eluate with a purity higher than 90%. The 15% ethanol eluate was collected and concentrated to 10ml by rotary evaporation.
[0028] (3) Put the concentrated solution of (2) in a refrigerato...
Embodiment 2
[0030] A method for isolating and purifying cordycepin from Cordyceps militaris culture fluid, comprising the following steps:
[0031] (1) Obtain 1L of Cordyceps militaris culture fluid, filter the culture fluid with filter paper, and then concentrate it to 20ml by rotary evaporation.
[0032] (2) Use the concentrated culture solution on the macroporous resin column D101, the column volume is 500ml, the elution solvent is water, 10% ethanol, 95% ethanol in sequence, the flow rate is 10ml / min, the elution solvent volume is 2L of water (200ml / bottle, 10 bottles), 10% ethanol 2.4L (200ml / bottle, 12 bottles), 95% ethanol 1L (collected together). The HPLC detection of the received elution solvent showed that cordycepin was concentrated in the 10% ethanol eluent with a purity higher than 90%. The 10% ethanol eluate was collected and concentrated to 10ml by rotary evaporation.
[0033] (3) Put the concentrated solution of (2) in a refrigerator at 4°C overnight to obtain cordycepin...
PUM
Property | Measurement | Unit |
---|---|---|
melting point | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com