A kind of paleobacterium mgj11 and its method for immobilizing heavy metal cadmium in soil

A technology of Pallidum and heavy metals, applied in the restoration of contaminated soil, methods based on microorganisms, botany equipment and methods, etc., can solve the problems of unrepaired cadmium-contaminated soil, etc., to enhance the stress tolerance of plants and promote the growth of plants. Effect of growth and reduction of cadmium accumulation

Active Publication Date: 2020-05-19
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, there is no report on the application of P. pallidum in PGPR to plant roots to remediate cadmium-contaminated soil

Method used

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  • A kind of paleobacterium mgj11 and its method for immobilizing heavy metal cadmium in soil
  • A kind of paleobacterium mgj11 and its method for immobilizing heavy metal cadmium in soil
  • A kind of paleobacterium mgj11 and its method for immobilizing heavy metal cadmium in soil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1 contains the bacterial agent of pale bacillus MGJ11

[0027] The strain was isolated from the soybean root system at the edge of the Panzhihua vanadium-titanium magnet tailings pond, and it was found that it had strong tolerance to cadmium and could secrete the plant growth hormone 3-indoleacetic acid (IAA). The plant rhizosphere inoculated with the bacteria can produce the plant growth hormone IAA to promote plant growth, reduce the enrichment of Cd in the soil by plants to alleviate the stress of cadmium on plants, thereby fixing the cadmium in the plant rhizosphere soil, enhancing Stress tolerance of plants.

[0028] The strain was identified as Ochrobacterium sp., numbered MGJ11, and preserved in the General Microbiology Center of China Committee for Microbial Culture Collection (CGMCC for short, address: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, China Institute of Microbiology, Academy of Sciences, Zip code: 100101), the date of de...

Embodiment 2

[0031] Example 2 Microorganism fixes soil heavy metal cadmium and indoor test effect

[0032] The bacterium agent described in embodiment 1 is diluted 500 times.

[0033] The greenhouse pot experiment was carried out with vermiculite added with heavy metal cadmium as the soil matrix. Soybeans were planted in cadmium-containing soil, and the diluted bacterial agent was sprayed into the soil around the roots of soybean seedlings, so that the microbial concentration was 10 9 ~10 10 Bacteria cells / plants, and soybean seedlings not inoculated with Paleobacterium pallidum (MGJ11) were used as a negative control, and nitrogen-containing nutrient solution (KNO 3 ,KH 2 PO 4 0.2gL -1 , KCl 0.5gL -1 , CaSO 4 2H 2 O 0.2gL -1 , CaCO 3 2gL -1 , MgSO 4 ·7H 2 O0.2g, Fe EDTA, H 3 BO 3 1mgL -1 , ZnSO 4 ·7H 2 O1mg L -1 , CuSO 4 ·5H 2 O 0.5mgL -1 , MnCl 2 4H 2 O0.5mgL -1 , Na 2 MoO 4 2H 2 O 0.1mg L -1 ), and each treatment was replicated three times (that is, three ...

Embodiment 3

[0042] Example 3 Microorganism fixes soil heavy metal cadmium and outdoor test effect

[0043] The soil from industrial and mining areas containing cadmium is used as in-situ remediation soil to conduct outdoor remediation effect tests; soybeans are used as remediation plants, but the application to this plant is not limited.

[0044] Dig holes in industrial and mining areas, apply appropriate amount of base fertilizer, and then sow soybeans directly in the industrial and mining cover soil. The depth of seed sowing is 1-2cm from the soil surface. After the seeds grow 2-3cm from the soil surface, dilute the bacterial agent 500 times and then irrigate the roots. , the concentration is 10 9 ~10 10 Bacterial cells / strain; water and topdress timely according to the growth status of soybeans.

[0045]After 38 days, the survival rate of the bean sprouts was 90%, and the growth was good, and it was obvious that the growth of soybean plants inoculated with Ochrobacterium sp. MGJ11 wa...

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Abstract

The invention relates to the technical field of microorganism and environment treatment, and particularly discloses an ochrobacterum sp. MGJ11, which is deposited with the CGMCC at Sep 16th, 2015 and is assigned the accession number CGMCC No.11385. The invention also discloses a method of immobilizing heavy metal cadmium in soil with the ochrobacterum sp. MGJ11, wherein the method particularly includes the steps of applying the ochrobacterum sp. MGJ11 to rhizosphere of plants, or mixing powder of the ochrobacterum sp. MGJ11 with seeds and planting the seeds, wherein the concentration of the inoculated microorganism is not less than 10<9> bacterial cells in every plant.

Description

technical field [0001] The invention relates to the technical field of microbes and environmental treatment, in particular, to a method for fixing soil heavy metal cadmium by using the bacillus pallidum MGJ11 Background technique [0002] Sewage irrigation in farmland, application of solid waste, extensive use of chemical fertilizers and pesticides, and human activities such as mining and smelting have caused soil to be more and more polluted by heavy metals. Among them, cadmium (Cd) pollution is particularly obvious (Jiang Chunyu et al., Biological characteristics of a lead-cadmium resistant strain WS34 and its strengthening effect on phytoremediation of lead-contaminated soil[J]. Environmental Science Journal 2008, 28(10) .1961-1968). Cadmium is one of the heavy metal elements that are highly toxic to plants and humans. Long-term exposure to a certain dose of cadmium can cause kidney damage, which can lead to osteoporosis and softening, and can lead to cancer in severe c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F11/08B09C1/10A01N63/20A01P21/00C12R1/01
Inventor 余秀梅李杨昕李艳梅康夏陈强辜运富陈翠平谢雨歆崔永亮
Owner SICHUAN AGRI UNIV
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