Method for determination of content of verapamil hydrochloride in drug by using electrogenerated chemiluminescence behavior of composite modifier-supported pyrolytic graphite electrode

A technology of verapamil hydrochloride and pyrolytic graphite, which is applied in electrical excitation analysis, material excitation analysis, etc., can solve the problems of polluted environment and operation, low utilization efficiency and high test cost, and achieves easy cracking and high sensitivity. , the effect of a wide linear range

Inactive Publication Date: 2016-06-15
GUANGXI UNIVERSITY OF TECHNOLOGY
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Problems solved by technology

In summary, when using the drop method to measure verapamil hydrochloride, there is Ru(bpy) 3 2+ Large amount of use, low utilization efficiency, high test cost, environmental pollution and inconvenient operation, etc., and the measurement effect needs to be further improved

Method used

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  • Method for determination of content of verapamil hydrochloride in drug by using electrogenerated chemiluminescence behavior of composite modifier-supported pyrolytic graphite electrode
  • Method for determination of content of verapamil hydrochloride in drug by using electrogenerated chemiluminescence behavior of composite modifier-supported pyrolytic graphite electrode
  • Method for determination of content of verapamil hydrochloride in drug by using electrogenerated chemiluminescence behavior of composite modifier-supported pyrolytic graphite electrode

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Embodiment 1

[0040] Embodiment 1: a kind of method utilizing composite modifier loaded pyrolytic graphite electrode electrochemiluminescence behavior to measure verapamil hydrochloride content in medicine, comprises the following steps:

[0041] (1) Preparation of nano-titanium dioxide sol

[0042] 4mLTiCl 4 Into 100mL ethanol solution, take 20mLTiCl 4 -EtOH solution and adjust the pH=8.8 with 37mL 2.5% ammonia water, filter with suction, wash the filter cake with deionized water until neutral, wash with 30mLH 2 o 2 Dissolved, then added 5mL of silica sol and 65mL of water, and refluxed at 95°C for 4h.

[0043] (2) Preparation of Nano-Ag

[0044] Using silver nitrate as precursor and PVP as stabilizer and reducing agent, take 0.169g of silver nitrate in 50mL of ethanol solution with rapid stirring, and disperse at 40°C for 10min; meanwhile, add 1g of PVP into 50mL of ethanol and dissolve at 60 ℃ for 10 minutes, then silver nitrate solution was added dropwise to the PVP solution, and r...

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Abstract

The invention relates to a method for determination of the content of verapamil hydrochloride in a drug by using an electrogenerated chemiluminescence behavior of a composite modifier-supported pyrolytic graphite electrode; with use of an auxiliary film forming property of TiO2 sol and silica sol and a good electrocatalytic property of ZnO@Ag, a TiO2 sol/ZnO@Ag/silica sol composite modifier is prepared; the surface of a pyrolytic graphite electrode is supported with bipyridine ruthenium by a sensor modification technology, an electrogenerated chemiluminescence sensor which is used for determination of verapamil hydrochloride and has good stability and high sensitivity is prepared, and simple and rapid determination of verapamil hydrochloride is achieved. Under optimal test conditions, 1.0*10<-7>-1.0*10<-4> mol/L verapamil hydrochloride has a linear relationship (R<2>=0.9731) with the relative luminescence intensity, and a detection limit (S/N=3) is 7.11*10<-9> mol/L. The method has relatively high selectivity and sensitivity for determination of verapamil hydrochloride, and realizes repeated use of bipyridine ruthenium.

Description

technical field [0001] The invention relates to a method for measuring the content of verapamil hydrochloride in medicine, in particular to a method for measuring the content of verapamil hydrochloride in medicine by using the electrochemiluminescence behavior of a pyrolytic graphite electrode loaded with a composite modifier. Background technique [0002] Verapamil hydrochloride (Verapamilhydrochloride) is a calcium ion antagonist, which can selectively dilate coronary arteries and increase coronary flow; it can inhibit myocardial excitability and atrioventricular conduction, and is commonly used in clinical antiarrhythmia, antiangina pectoris and treatment of high blood pressure. Drugs for blood pressure disease. At present, in the method for determining verapamil hydrochloride, mainly based on high performance liquid chromatography, also includes reversed phase high performance liquid phase-fluorescence detection chromatography, capillary electrophoresis, flow injection c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/66
CPCG01N21/66
Inventor 李彦青罗应李利军程昊黄文艺冯军孔红星魏慧丹史智鹏
Owner GUANGXI UNIVERSITY OF TECHNOLOGY
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