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Preparation method and application of dual-purpose probe integrating solid-phase micro-extraction fibers and electrochemical detection working electrodes

A technology for extracting fibers and working electrodes, which is applied in the field of analytical chemistry, can solve problems affecting the sensitivity and accuracy of results of analytical methods, achieve good biocompatibility and environmental stability, good environmental stability, and reduce the cost of use

Active Publication Date: 2016-06-22
BEIJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The traditional solid-phase microextraction technology needs to use the method of solvent analysis to resolve the extracted analytes into the solvent for further analysis. The analysis process will cause a series of disadvantages such as dilution of the sample to be tested and changes in the state of the analyte. Affect the sensitivity of the analytical method and the accuracy of the results

Method used

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  • Preparation method and application of dual-purpose probe integrating solid-phase micro-extraction fibers and electrochemical detection working electrodes
  • Preparation method and application of dual-purpose probe integrating solid-phase micro-extraction fibers and electrochemical detection working electrodes
  • Preparation method and application of dual-purpose probe integrating solid-phase micro-extraction fibers and electrochemical detection working electrodes

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Effect test

Embodiment 1

[0032] Dual-function probe integrating solid-phase microextraction fiber and electrochemical detection working electrode

[0033] (1) Take a carbon fiber (7μm in diameter) and use 30% HNO 3 solution, acetone, ethanol, double distilled water to ultrasonically clean the carbon fiber for 10 minutes, and dry at room temperature;

[0034] (2) Take a cleaned and dried carbon fiber and connect it with copper wire through silver conductive glue, and insert it into a glass capillary tube with a 10 μm tip drawn at one end;

[0035] (3) Use epoxy glue to seal and fix the carbon fiber and copper wire at both ends of the glass capillary, and fully dry at room temperature;

[0036] (4) Cut the carbon fiber at the tip of the capillary to obtain a length of about 1.0cm, and use it as a working electrode at 0.5mol / LH 2 SO 4 Cyclic scan activation in the solution in the range of -0.2 ~ 1.2V, to obtain a stable curve;

[0037] (5) After the activated electrode is cleaned and dried at room te...

Embodiment 2

[0040] The dopamine in the aqueous solution was extracted by using the dual-functional probe integrating the solid-phase microextraction fiber and the electrochemical detection working electrode prepared in Example 1, and then used the probe as the working electrode for electrochemical detection.

[0041] Immerse the solid-phase microextraction-electrochemical detection working electrode dual-function probe into 5 mL of 20 μg / L dopamine solution (solvent is PBS, pH value is 7.40), and extract at room temperature for 20 min. After the extraction, rinse the surface quickly with distilled water, and The probe is used as the working electrode, the platinum wire is the counter electrode and the Ag / AgCl is the reference electrode. The three electrodes are immersed in the PBS buffer solution (pH value is 7.40), and connected to the CHI852D electrochemical work, at -0.2 The cyclic voltammetry test was carried out in the range of ~0.6V, and the scan rate was 0.05V / s.

[0042] image 3...

Embodiment 3

[0044] Utilize the dual-function probe that integrates solid-phase microextraction fiber and electrochemical detection working electrode prepared in Example 1 to extract the mixed solution of dopamine and 5-hydroxytryptamine in aqueous solution, and then use this probe as a working electrode for electrochemistry chemical detection.

[0045] Immerse the solid-phase microextraction-electrochemical detection dual-functional probe of the working electrode into 5mL of 20μg / L dopamine + 20μg / L 5-serotonin solution (the solvent is PBS, the pH value is 7.40), and extract at room temperature for 20min. After the extraction, use Rinse the surface quickly with distilled water, use the probe as the working electrode, platinum wire as the counter electrode and Ag / AgCl as the reference electrode, immerse the three electrodes in PBS buffer solution (pH value is 7.40), and work electrochemically with CHI852D Connected to each other, carry out cyclic voltammetry test in the range of -0.4 ~ 0.6...

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Abstract

The invention relates to a preparation method and an application of a dual-purpose probe integrating solid-phase micro-extraction fibers and electrochemical detection working electrodes, and belongs to the field of analysis chemicals. The preparation method comprises the following steps of firstly, adhering carbon fibers and a copper conducting wire by silver conductive adhesive, inserting into a capillary tube of which one end is drawn into a tip end, sealing the two ends of the capillary tube by epoxy resin adhesive, enabling the carbon fiber to expose with 1-20mm out of the glass capillary tube, and polymerizing a polypyrrole-graphene quantum dot copolymerizing coating to the surface of carbon fiber by an electrochemical polymerizing method, so as to obtain the dual-purpose probe integrating the solid-phase micro-extraction fibers and the electrochemical detection working electrodes. The preparation method has the advantages that by integrating the solid-phase micro-extraction fibers and the electrochemical detection working electrodes, the direct online detection is realized without solvent dissolving after extraction, and a series of problems of sample thinning, sample state change and the like in the solvent dissolving after the traditional solid-phase micro-extraction are improved; the preparation method is simple, the environment-friendly effect is realized, the detection sensitivity is high, and the application prospect in the fields of biological analysis, environment analysis and the like is broad.

Description

technical field [0001] The invention relates to a preparation method and application of a dual-function probe integrating a solid-phase microextraction fiber and an electrochemical detection working electrode, and belongs to the technical field of analytical chemistry. Background technique [0002] Solid-phase microextraction (SPME) technology is a new green and environmentally friendly sample pretreatment technology that integrates sampling, extraction, concentration, and sample injection. It is easy to operate, saves time and effort, and does not require solvents. It has been rapidly developed and widely used in many scientific fields such as , drug analysis and so on. The solid-phase microextraction method is significantly different from other sample pretreatment techniques. It is a solvent-free, fast, miniature, and sensitive microextraction technique that can extract a very small amount of analytes from biological systems or environmental systems without affecting The ...

Claims

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Application Information

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IPC IPC(8): G01N27/30G01N27/48G01N1/40
CPCG01N1/40G01N27/308G01N27/48
Inventor 汪夏燕陈永佳王琳郭广生
Owner BEIJING UNIV OF TECH
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