Preparation method and application of dual-purpose probe integrating solid-phase micro-extraction fibers and electrochemical detection working electrodes

A technology for extracting fibers and working electrodes, which is applied in the field of analytical chemistry, can solve problems affecting the sensitivity and accuracy of results of analytical methods, achieve good biocompatibility and environmental stability, good environmental stability, and reduce the cost of use

Active Publication Date: 2016-06-22
BEIJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The traditional solid-phase microextraction technology needs to use the method of solvent analysis to resolve the extracted analytes into the solvent for further analysis. The analysis process w

Method used

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  • Preparation method and application of dual-purpose probe integrating solid-phase micro-extraction fibers and electrochemical detection working electrodes
  • Preparation method and application of dual-purpose probe integrating solid-phase micro-extraction fibers and electrochemical detection working electrodes
  • Preparation method and application of dual-purpose probe integrating solid-phase micro-extraction fibers and electrochemical detection working electrodes

Examples

Experimental program
Comparison scheme
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Example Embodiment

[0031] Example 1

[0032] A dual-function probe integrating solid-phase micro-extraction fiber and electrochemical detection working electrode

[0033] (1) Take a carbon fiber (7μm in diameter) and use 30% HNO respectively 3 The carbon fiber was ultrasonically cleaned with solution, acetone, ethanol, and double distilled water for 10 minutes, and dried at room temperature;

[0034] (2) Take a cleaned and dried carbon fiber and copper wire connected by silver conductive glue, and insert it into a glass capillary with a 10μm tip drawn at one end;

[0035] (3) Use epoxy glue to seal and fix the carbon fiber and copper wire at both ends of the glass capillary, and fully dry at room temperature;

[0036] (4) Cut the carbon fiber at the tip of the capillary to obtain a length of about 1.0cm, and use it as a working electrode at 0.5mol / LH 2 SO 4 Cycle scanning activation in the range of -0.2~1.2V in the solution to obtain a stable curve;

[0037] (5) After the activated electrode is cleaned and...

Example Embodiment

[0039] Example 2

[0040] The bifunctional probe that integrates the solid-phase micro-extraction fiber and the electrochemical detection working electrode prepared in Example 1 was used to extract dopamine in the aqueous solution, and then the probe was used as the working electrode for electrochemical detection.

[0041] The solid phase microextraction-electrochemical detection working electrode dual-function probe is immersed in 5mL20μg / L dopamine solution (solvent is PBS, pH value is 7.40), and it is extracted at room temperature for 20min. After the extraction is completed, quickly rinse the surface with distilled water. This probe is used as the working electrode, platinum wire is the counter electrode and Ag / AgCl is the reference electrode. The three electrodes are immersed in the PBS buffer solution (pH value is 7.40) and connected to the CHI852D electrochemical work, at -0.2 The cyclic voltammetry test is performed in the range of ~0.6V, and the scan rate is 0.05V / s.

[004...

Example Embodiment

[0043] Example 3

[0044] The bifunctional probe that integrates the solid-phase microextraction fiber and the electrochemical detection working electrode prepared in Example 1 was used to extract the mixed solution of dopamine and serotonin in the aqueous solution, and then the probe was used as the working electrode for electrolysis. Chemical testing.

[0045] Immerse the solid-phase microextraction-electrochemical detection working electrode dual-function probe into 5mL20μg / L dopamine+20μg / L serotonin solution (solvent is PBS, pH value is 7.40), and extract at room temperature for 20min. After the extraction, use Rinse the surface quickly with distilled water, use this probe as the working electrode, platinum wire as the counter electrode and Ag / AgCl as the reference electrode, immerse the three electrodes in the PBS buffer solution (pH 7.40), and work with CHI852D electrochemically Phase connection, cyclic voltammetry test in the range of -0.4 ~ 0.6V, the scan rate is 0.05V / s....

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Abstract

The invention relates to a preparation method and an application of a dual-purpose probe integrating solid-phase micro-extraction fibers and electrochemical detection working electrodes, and belongs to the field of analysis chemicals. The preparation method comprises the following steps of firstly, adhering carbon fibers and a copper conducting wire by silver conductive adhesive, inserting into a capillary tube of which one end is drawn into a tip end, sealing the two ends of the capillary tube by epoxy resin adhesive, enabling the carbon fiber to expose with 1-20mm out of the glass capillary tube, and polymerizing a polypyrrole-graphene quantum dot copolymerizing coating to the surface of carbon fiber by an electrochemical polymerizing method, so as to obtain the dual-purpose probe integrating the solid-phase micro-extraction fibers and the electrochemical detection working electrodes. The preparation method has the advantages that by integrating the solid-phase micro-extraction fibers and the electrochemical detection working electrodes, the direct online detection is realized without solvent dissolving after extraction, and a series of problems of sample thinning, sample state change and the like in the solvent dissolving after the traditional solid-phase micro-extraction are improved; the preparation method is simple, the environment-friendly effect is realized, the detection sensitivity is high, and the application prospect in the fields of biological analysis, environment analysis and the like is broad.

Description

technical field [0001] The invention relates to a preparation method and application of a dual-function probe integrating a solid-phase microextraction fiber and an electrochemical detection working electrode, and belongs to the technical field of analytical chemistry. Background technique [0002] Solid-phase microextraction (SPME) technology is a new green and environmentally friendly sample pretreatment technology that integrates sampling, extraction, concentration, and sample injection. It is easy to operate, saves time and effort, and does not require solvents. It has been rapidly developed and widely used in many scientific fields such as , drug analysis and so on. The solid-phase microextraction method is significantly different from other sample pretreatment techniques. It is a solvent-free, fast, miniature, and sensitive microextraction technique that can extract a very small amount of analytes from biological systems or environmental systems without affecting The ...

Claims

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Application Information

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IPC IPC(8): G01N27/30G01N27/48G01N1/40
CPCG01N1/40G01N27/308G01N27/48
Inventor 汪夏燕陈永佳王琳郭广生
Owner BEIJING UNIV OF TECH
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