Culture medium for identifying group-B streptococcus

Abstract

The invention discloses a culture medium for identifying group-B streptococcus. The culture medium in per 100 ML water comprises, by mass, 3 g of proteose peptone, 0.2 g of KH2PO4, 0.4 g of Na2HPO4, 4 g of crude rice flour, 1.3 g of agar, 0.2 g of antibiotic, 0.2 g of brain-heart baptist extraction liquid medium and 2 g of horse sera. According to the culture medium, scribing detection can be directly carried out through a sampled to-be-detected swab; compared with the condition that pure bacterial strains need to be identified in the prior art, the detection accuracy and the detection efficiency of the group-B streptococcus are improved through the culture medium; An application method is convenient to operate, and special instruments and devices are not required; the culture medium is suitable for hospital and particularly suitable for applications of basic medical institutions, sampling work of samples is convenient, and meanwhile the detection effect of the group-B streptococcus is guaranteed.

Description

technical field [0001] The invention relates to a microorganism culture medium, in particular to a group B streptococcus selective culture medium. Background technique [0002] Group B Streptococcus (GBS), also known as Group B Streptococcus or Streptococcus agalactiae, belongs to Gram-positive bacteria and is a normal flora that inhabits the human gastrointestinal tract and lower reproductive tract. Since the 1970s, GBS has gradually become one of the important pathogenic bacteria of severe neonatal infectious diseases, which has attracted widespread attention all over the world. GBS infection is the main cause of perinatal intrauterine infection, neonatal pneumonia, sepsis, meningitis and other serious infections. It can cause serious consequences such as premature birth, fetal growth retardation (low birth weight infant), premature rupture of membranes and late miscarriage, and occupies an important position in perinatal medicine. [0003] At present, there are two main...

AI Technical Summary

Problems solved by technology

However, there are a variety of bacteria in the reproductive tract and rectum of pregnant women, and the current culture medium cannot effectively inhibit the growth of other bacteria other than GBS. These bacteria grow quickly, which often inhibits the growth of GBS bacteria, resulting in missed detection of GBS bacteria

For this reason, when using the existing Group B Streptococcus identification medium for identification of Group B Streptococcus in clinical testing, it is necessary to purify, multiply and culture the sampled strains before identification, which is cumbersome and wastes time and manpower

Currently, the Group B streptococcus screening medium of Mérieux Company, which is used clinically, mainly adopts the method of substrate color development, which is expensive and expensive.

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