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Inactivated vaccine prepared through mink pseudomonas aeruginosa strain SD17

An inactivated vaccine, Pseudomonas aeruginosa technology, applied in the direction of veterinary vaccines, vaccines, medical preparations containing active ingredients, etc., can solve the problems of weak immunogenicity, low immune cross protection, etc. Mortality reduction, epidemic and transmission prevention, economic loss reduction effect

Inactive Publication Date: 2016-07-06
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention provides an inactivated vaccine prepared by Pseudomonas aeruginosa SD17 strain from mink, so as to solve the problems of weak immunogenicity of existing vaccine strains and low immune cross-protection between serotypes in the research and development of Pseudomonas aeruginosa inactivated vaccines question

Method used

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  • Inactivated vaccine prepared through mink pseudomonas aeruginosa strain SD17
  • Inactivated vaccine prepared through mink pseudomonas aeruginosa strain SD17
  • Inactivated vaccine prepared through mink pseudomonas aeruginosa strain SD17

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] The Pseudomonas aeruginosa strain G type SD17 was deposited in the China Center for Type Culture Collection (CCTCC) of Wuhan University in Wuhan, China on February 22, 2016, and the preservation number is CCTCC NO: M2016069.

[0014] production medium

[0015] Hexadecanetrimethylammonium bromide agar medium (NAC) preparation: Weigh 4g of NAC powder and add it to 100mL of distilled water, mix thoroughly, sterilize at 121°C for 15min, cool to 50°C to 60°C, pour the plate for later use.

[0016] Preparation of improved nutrient broth medium (NB): Weigh 5g of NB powder into 100mL of distilled water, and sterilize at 121°C for 15min. It is prepared by adding a nutrient broth medium with a final concentration of 2% fetal bovine serum before use, and mixing evenly.

[0017] Fermentation medium preparation: 2% peptone, 0.6% beef extract, 0.5% sodium chloride, 0.5% glucose, made with deionized water. 2% of healthy fetal bovine serum was added.

[0018] 1.1 Isolation and purif...

Embodiment 2

[0047] Application of mink Pseudomonas aeruginosa G type SD17 strain in preparation of inactivated vaccine.

[0048] The mink Pseudomonas aeruginosa G type SD17 strain was taken, activated and inoculated into the medium respectively, the bacterial liquid was collected after cultivation, inactivated by formaldehyde solution, mixed with propolis adjuvant to prepare inactivated propolis vaccine.

[0049] The preferred operation steps are as follows:

[0050] 2.1 Strain selection

[0051] The strain used for making seedlings was Pseudomonas aeruginosa G type SD17 strain of mink, its colony morphology, cell characteristics, biochemical characteristics, and culture characteristics were all stable, and it had strong pathogenicity to mink. 1.0mL bacterial liquid (8.0×10 6 CFU / mL) injection can cause 100% of 21 to 42 days old mink disease. The immunogenicity of mink Pseudomonas aeruginosa G type SD17 strain is good, and the minimum immune dose should be ≥4.0×10 9 .

[0052] 2.2 Pre...

Embodiment 3

[0092] Application of mink Pseudomonas aeruginosa G type SD17 strain in preparation of inactivated vaccine.

[0093] Take Pseudomonas aeruginosa G type SD17 strain from mink, inoculate the culture medium after activation, collect the bacterial liquid after culture, inactivate with formaldehyde solution, and mix with water adjuvant to prepare inactivated vaccine.

[0094] The preferred operation steps are as follows:

[0095] 2.1 Strain selection

[0096] The strain used for making seedlings was Pseudomonas aeruginosa G type SD17 strain of mink, its colony morphology, cell characteristics, biochemical characteristics, and culture characteristics were all stable, and it had strong pathogenicity to mink. 1.0mL bacterial liquid (8.0×106 CFU / mL) injection can cause 100% of 21 to 42 days old mink disease. The immunogenicity of mink Pseudomonas aeruginosa G type SD17 strain is good, and the minimum immune dose should be ≥4.0×10 9 .

[0097] 2.2 Preparation of strains for productio...

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Abstract

The invention provides an inactivated vaccine prepared through a mink pseudomonas aeruginosa strain SD17. The inactivated vaccine comprises antigen and vaccine adjuvant, wherein the antigen is an inactivated pseudomonas aeruginosa strain SD17 and has the preservation number of CCTCC NO:M2016069. The prepared inactivated vaccine is safe and reliable, can provide effective homologous challenge protection and can produce strong immunity after immunity, the morbidity and death rate of vaccinated mink groups are both remarkably decreased, the prevalence and transmission of mink hemorrhagic pneumonia can be effectively prevented, the economic loss caused by the disease to the fur-bearing animal breeding industry is reduced, and wide application prospects are achieved.

Description

technical field [0001] The invention belongs to the technical field of vaccine preparation, in particular to an inactivated vaccine prepared from mink Pseudomonas aeruginosa SD17 strain. Background technique [0002] Mink hemorrhagic pneumonia, also known as mink Pseudomonas pneumonia, is an acute infectious disease that mainly occurs during the moulting season of mink from August to November every year. It is characterized by hemorrhagic pneumonia and acute death. Nostrils flowed out of red foamy liquid and other symptoms. Post-mortem examination showed that the entire lung lobe was enlarged with diffuse hemorrhage and sepsis. The disease occurs all over the world and can cause 20%-40% mortality in farms every year. It is an important bacterial infectious disease that endangers the fur animal breeding industry. [0003] The disease pathogen is Pseudomonas aeruginolsa (Pseudomonasaeruginolsa) in Pseudomonas (Pseudomonadaceae) Pseudomonas (Pseudomonas), also known as Pseudom...

Claims

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Application Information

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IPC IPC(8): A61K39/104A61K39/39A61P11/00A61P31/04A61P7/04
CPCA61K39/104A61K39/39A61K2039/521A61K2039/552A61K2039/55505A61K2039/55588
Inventor 单虎张洪亮盖春云黄娟张传美秦志华杨瑞梅秦晓冰
Owner QINGDAO AGRI UNIV
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