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Cultivation medium for liquid strains of black fungi and method for preparing cultivation medium

A technology of liquid bacteria and culture medium, applied in the field of bioengineering, can solve the problems of inability to carry out standardization and factory production, slow growth of mycelium, insufficient dissolution of raw materials, etc. The effect of economic and social benefits

Inactive Publication Date: 2016-07-13
LIAONING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The existing black fungus liquid culture medium is mainly prepared from corn flour, potatoes, bran, peptone, yeast extract, white sugar and inorganic salts, and there are problems such as many raw materials and high costs.
In the preparation process, long-time cooking is required, and there are problems such as insufficient dissolution of raw materials, many particulate matter, long sterilization time, and complicated preparation process.
The prepared liquid strains have disadvantages such as slow growth of mycelium and long cultivation period, which makes it impossible to carry out standardization and factory production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A preparation method of black fungus liquid culture medium, carried out according to the following steps:

[0029] First weigh the components with the following mass percentage concentrations: 2% corn distiller's grain waste liquid, 1% sucrose, 0.05% potassium dihydrogen phosphate and 0.025% magnesium sulfate, and then add the corn distiller's grain waste liquid into a 70-80°C Stir in 15L hot water and make it dissolve, boil to remove the grease contained therein to obtain the first aqueous solution,

[0030] adding sucrose, potassium dihydrogen phosphate and magnesium sulfate into 15L hot water at 70-80°C, stirring and dissolving to obtain a second aqueous solution,

[0031] uniformly mixing the first aqueous solution and the second aqueous solution to obtain a mixed solution,

[0032] Adjust the pH value of the mixed solution to 6.0 with sodium hydroxide,

[0033] Put the mixed solution after adjusting the pH value into a 50L fermenter for sterilization. The sterili...

Embodiment 2

[0036] A preparation method of black fungus liquid culture medium, carried out according to the following steps:

[0037] First weigh the components with the following mass percentage concentrations: 3% corn distiller's grain waste liquid, 2% sucrose, 0.1% potassium dihydrogen phosphate and 0.05% magnesium sulfate, and then add the corn distiller's grain waste liquid into a 70-80°C Stir in 15L hot water and make it dissolve, boil to remove the grease contained therein to obtain the first aqueous solution,

[0038] adding sucrose, potassium dihydrogen phosphate and magnesium sulfate into 15L hot water at 70-80°C, stirring and dissolving to obtain a second aqueous solution,

[0039] uniformly mixing the first aqueous solution and the second aqueous solution to obtain a mixed solution,

[0040] Adjust the pH value of the mixed solution to 6.0 with sodium hydroxide,

[0041] Put the mixed solution after adjusting the pH value into a 50L fermenter for sterilization. The steriliza...

Embodiment 3

[0044] A preparation method of black fungus liquid culture medium, carried out according to the following steps:

[0045] First weigh the components with the following mass percentage concentrations: 4% corn distiller's grain waste liquid, 3% sucrose, 0.15% potassium dihydrogen phosphate and 0.075% magnesium sulfate, and then add the corn distiller's grain waste liquid into a 70-80°C Stir in 15L hot water and make it dissolve, boil to remove the grease contained therein to obtain the first aqueous solution,

[0046] adding sucrose, potassium dihydrogen phosphate and magnesium sulfate into 15L hot water at 70-80°C, stirring and dissolving to obtain a second aqueous solution,

[0047] uniformly mixing the first aqueous solution and the second aqueous solution to obtain a mixed solution,

[0048] Adjust the pH value of the mixed solution to 6.0 with sodium hydroxide,

[0049] Put the mixed solution after adjusting the pH value into a 50L fermenter for sterilization. The sterili...

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PUM

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Abstract

The invention discloses a cultivation medium for liquid strains of black fungi and a method for preparing the cultivation medium.The cultivation medium and the method have the advantages that the cultivation medium contains few components and is easy to obtain and low in cost, waste liquid of distillers' grains generated in procedures for producing alcohol by the aid of fermentation processes is utilized, to be more specific, the waste liquid of the corn distillers' grains is an important composition portion of the cultivation medium and contains 92-95% of water, remaining 5-8% of dry substances in the waste liquid contain abundant nutrition ingredients such as carbohydrate, proteins, celluloses, pentosan, caramel, fat, abundant vitamins B and auxin, and accordingly, nutrition ingredients necessary for growth of the liquid strains of the black fungi can be provided by the cultivation medium; the method for preparing the cultivation medium is relatively simple and includes controllable, simple and convenient operation steps, the problem of pollution due to the waste liquid drained from an alcohol plant can be solved if the cultivation medium is used for cultivating the liquid strains of the black fungi, and the cost can be reduced to a great extent; mycelia of the black fungi can grow fast and are high in activity and content, a formula for the cultivation medium for the liquid strain is favorable for standard production, and the cultivation medium and the method have obvious economic and social benefits.

Description

technical field [0001] The invention relates to the field of bioengineering, in particular to a black fungus liquid culture medium and a preparation method thereof. Background technique [0002] Black fungus belongs to Basidiomycetes, Auricularia, Auriculariaceae, Auricularia genus, commonly known as fungus, fungus, fungus, house fungus, cloud fungus, black cabbage, etc. The black fungus is delicate in texture, crisp and refreshing, and rich in nutrition. According to modern scientific analysis, every 100 grams of dry product contains 10.6 grams of protein, 0.2 grams of fat, 65 grams of carbohydrates, 7 grams of crude fiber, 375 mg of calcium, 201 mg of phosphorus, and 185 mg of iron. Contains vitamin B10.15mg, vitamin B20.55mg, niacin 2.7mg. Black fungus not only has high nutritional value, but also has high medicinal value. [0003] Alcohol production by fermentation is the main method used in the production of alcohol in various countries. After the alcohol is evaporate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G1/00
CPCC05B7/00C05G5/23C05D5/00C05F5/008C05F11/00
Inventor 谢鲲鹏佟少明宫正谢明杰
Owner LIAONING NORMAL UNIVERSITY
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