Method of utilizing aquatic product viscera and oyster shells to prepare organic fertilizer
A technology for organic fertilizers and aquatic products, applied in the field of processing and utilization of aquatic products, to achieve the effects of saving investment, increasing energy consumption, and saving energy costs
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Embodiment 1
[0029]Example 1: Insert the thermostable facultative anaerobic bacteria Bacillus methylotrophicus HL-4 preserved on the slant into the LB liquid medium cooled after sterilization, cultivate it at 55°C and 150 r / min for 12 hours, and prepare Bacillus methylotrophicus HL-4 seed liquid for later use ; Mix bran and water in a mass ratio of 1:1, and mix well to prepare bran medium. Bran culture is based on sterilization at 121°C for 30 minutes before use; the prepared Bacillusmethylotrophicus HL-4 seed solution is inserted into After sterilization, put the bran medium in the cooled bran medium and mix it evenly, then divide the bran medium into which the strains are inserted into the sterilized fermentation shallow pan, the thickness of the filling is 4cm, and the surface of the fermentation medium in the shallow pan is covered Sterilized wet gauze, and then put the tray on the shelf in the sterilized solid fermentation room to ferment, control the ambient temperature at 55°C, and f...
Embodiment 2
[0030] Example 2: Insert the thermostable facultative anaerobic bacteria Bacillus methylotrophicus HL-4 preserved on the slant into the LB liquid medium cooled after sterilization, and cultivate it at 55°C and 150 r / min for 12 hours, and prepare Bacillus methylotrophicus HL-4 seed liquid for later use ; Mix bran and water in a mass ratio of 1:1, and mix well to prepare bran medium. Bran culture is based on sterilization at 121°C for 30 minutes before use; the prepared Bacillusmethylotrophicus HL-4 seed solution is inserted into After sterilization, put the bran medium in the cooled bran medium and mix it evenly, then divide the bran medium into which the strains are inserted into the sterilized fermentation shallow pan, the thickness of the filling is 4cm, and the surface of the fermentation medium in the shallow pan is covered Sterilized wet gauze, and then put the tray on the shelf in the sterilized solid fermentation room to ferment, control the ambient temperature at 55°C, ...
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