Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Fish digestion-resistant peptide with oxidation resisting function, preparation method therefor and use of fish digestion-resistant peptide

A technology of anti-digestion and anti-oxidation, applied in the field of fish meat digestive-resistant peptide, and its preparation field, can solve the problems that have not yet been found, and achieve the effects of high social and economic benefits, simple preparation method and good anti-oxidation ability

Active Publication Date: 2016-07-20
BINZHOU WANJIA BIOTECH
View PDF2 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, many studies on active peptides using fish meat as raw materials have been reported at home and abroad. However, fish meat digestion-resistant peptides have not been found to use fish meat as raw materials. The in vitro digestion and absorption model is used to simulate its digestion and absorption, and the LS-MS / MS identification can Reporting of intact absorbed peptides

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Fish digestion-resistant peptide with oxidation resisting function, preparation method therefor and use of fish digestion-resistant peptide
  • Fish digestion-resistant peptide with oxidation resisting function, preparation method therefor and use of fish digestion-resistant peptide
  • Fish digestion-resistant peptide with oxidation resisting function, preparation method therefor and use of fish digestion-resistant peptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Cod Fish Meat Digestive Resistance Peptide with Antioxidant Function and Its Preparation

[0028] The preparation method of cod fish digestion-resistant peptide with antioxidant function is as follows:

[0029] (1) Wash 500g of cod meat with clear water, mince the fish with a grinder, then add 1500g of water 3.0 times the weight of the fish to the minced fish, place it in a water bath at 37°C for 15min, and then use a homogenizer to Homogenize for 3 minutes at 12000 rpm to obtain 2000 g of fish meat homogenate.

[0030] (2) Use 1mol / L NaOH or HCl to adjust the pH of the fish homogenate in step (1) to 7.0, adjust the temperature to 40°C, and then add animal protein hydrolysis compound enzyme, the mass ratio of enzyme to fish is 1:100 , enzymolysis for 3 hours, and then the hydrolyzate was boiled at 100°C to inactivate the enzyme for 10 minutes, centrifuged at 5000g for 15 minutes, and the supernatant was collected.

[0031] (3) Ultrafiltration is carried out ...

Embodiment 2

[0032] Example 2 Grass carp fish meat digestion-resistant peptide with antioxidant function and its preparation

[0033] The preparation method of the fish digestion-resistant peptide of grass carp with antioxidant function is as follows:

[0034] (1) Wash 300g of grass carp meat with clean water, grind the fish meat with a grinder, then add 600g of water 2.0 times the weight of the fish meat to the minced fish meat, place it in a water bath at 45°C for 10min, and then use a homogenizer to Homogenize for 2 minutes at 12000 rpm to obtain 900 g of fish meat homogenate.

[0035] (2) Use 1mol / L NaOH or HCl to adjust the pH of the fish homogenate in step (1) to 7.5, adjust the temperature to 40°C, and then add animal protein hydrolysis compound enzyme, the mass ratio of enzyme to fish is 1:200 , enzymatically hydrolyzed for 2 hours, then boiled the hydrolyzed solution at 100°C to inactivate the enzyme for 10 minutes, centrifuged at 5000g for 15 minutes, and collected the supernata...

Embodiment 3

[0037] Example 3 Fish Meat Digestive Resistance Peptide with Antioxidant Function of Silver Carp and Its Preparation

[0038] The preparation method of the fish digestion-resistant peptide of silver carp with antioxidant function is as follows:

[0039] (1) Wash 600g silver carp meat with clean water, grind the fish meat with a grinder, then add 1800g of water 3.0 times the weight of the fish meat to the minced fish meat, place it in a water bath at 50°C for 10min, and then use a homogenizer Homogenize for 5 minutes under the condition of 15000rpm to obtain 2400g of fish meat homogenate.

[0040] (2) Use 1mol / L NaOH or HCl to adjust the pH of the fish homogenate in step (1) to 8.0, adjust the temperature to 45°C, and then add animal protein hydrolysis compound enzyme, the mass ratio of enzyme to fish is 1:250 , enzymatically hydrolyzed for 3 hours, then boiled the hydrolyzate at 85°C to inactivate the enzyme for 20min, centrifuged at 7000g for 15min, and collected the superna...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a fish digestion-resistant peptide with an oxidation resisting function. The fish digestion-resistant peptide has the amino acid sequence shown in SEQ ID NO: 1-11. The fish digestion-resistant peptide is prepared through hydrolyzing fish protein by using an animal proteolysis compound enzyme, carrying out centrifugation, and extracting supernatant; and carrying out ultrafiltration on the supernatant by using an ultrafiltration membrane with the molecular weight cutoff of 3,000Da, collecting filtrate, and then, carrying out concentrating and freeze-drying. The fish digestion-resistant peptide with the oxidation resisting function, provided by the invention, has relatively good free-radical removing capacity and can still maintain very good oxidation resistance after the fish digestion-resistant peptide is subjected to digestion and absorption by a gastrointestinal tract; and furthermore, natural fishes serve as a raw material, so that the prepared fish digestion-resistant peptide is safe and non-toxic. According to the fish digestion-resistant peptide, the preparation method is simple, the problem in the industrialized production of the fish digestion-resistant peptide can be effectively solved, and thus the fish digestion-resistant peptide has relatively high social and economic benefits.

Description

technical field [0001] The invention belongs to the field of aquatic food processing, and in particular relates to a fish digestion-resistant peptide with anti-oxidation function, its preparation method and application. Background technique [0002] The production of reactive oxygen species (ROS) in the human body will cause oxidative damage to biological macromolecules, such as lipids, proteins, DNA and nucleic acids, and these oxidative damages will increase the risk of many diseases (cancer, cardiovascular disease, etc.) )happened. Supplementing additional antioxidants can help the body maintain the level of oxidative stress in the body and prevent the occurrence of aging and disease. Among them, natural antioxidants, such as fish digestion-resistant peptides, have attracted much attention due to their rich protein sources and their own safety. During the process of protein fermentation, enzymolysis or digestion, fish digestion-resistant peptides can be released from th...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07K7/06C07K7/08C12P21/06C12P21/02A61K8/64A61Q19/08
CPCA61K8/64A61Q19/08C07K7/06C07K7/08C12P21/02C12P21/06
Inventor 李博王波郭珊珊霍艳娇罗永康
Owner BINZHOU WANJIA BIOTECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com