Molecular marker closely linked with melon pseudoperonospora cubensis resisting gene and application thereof
A downy mildew resistance and molecular marker technology, applied in the field of agricultural biology, can solve the problems of long genetic distance and difficult application of molecular marker-assisted breeding, so as to shorten the breeding cycle and improve the selection accuracy
Active Publication Date: 2016-08-03
XINJIANG AGRI SCI ACAD CANTALOUPE RES CENT
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Abstract
The invention discloses a molecular marker closely linked with a melon pseudoperonospora cubensis resisting gene and the application thereof. Based on the seedling-stage resource pseudoperonospora cubensis resistance identification and selection principle and the prior art, a melon resource PI438685 with high pseudoperonospora cubensis resistance is selected as the high-resistance resource, a melon pseudoperonospora cubensis anti-fever F2-generation group is established by means of the high-resistance resource PI438685 and the susceptible farm variety Clarke, the molecular marker SSR-666230 linked with the PI438685 pseudoperonospora cubensis resisting gene is found by means of well-known heredity software, and the genetic distance between the marker and the resistance gene is 2.66 cm. By extracting genome DNA of a single melon plant and conducting PCR amplification with the marker SSR-666230, whether a unique 230bp specific band appears is detected, it is predicted that the single melon plant has pseudoperonospora cubensis resistance if yes, the technical effects of shortening breeding period and improving selection accuracy during pseudoperonospora cubensis resisting strain breeding are realized, and practical value is high.
Application Domain
Microbiological testing/measurementDNA/RNA fragmentation
Technology Topic
Agricultural scienceHigh resistance
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