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Molecular marker closely linked with melon pseudoperonospora cubensis resisting gene and application thereof

A downy mildew resistance and molecular marker technology, applied in the field of agricultural biology, can solve the problems of long genetic distance and difficult application of molecular marker-assisted breeding, so as to shorten the breeding cycle and improve the selection accuracy

Active Publication Date: 2016-08-03
XINJIANG AGRI SCI ACAD CANTALOUPE RES CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The molecular marker research on the source of resistance was only reported by the Zhengzhou Fruit Tree Institute of the Chinese Academy of Agricultural Sciences respectively against the parent DM3 and PI414723, and found 1 SRAP marker and 3 SSR markers linked to it, but the genetic distance is too far, and the molecular markers Difficult to apply in assisted breeding

Method used

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  • Molecular marker closely linked with melon pseudoperonospora cubensis resisting gene and application thereof
  • Molecular marker closely linked with melon pseudoperonospora cubensis resisting gene and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0062] Embodiment one: the molecular marker closely linked with melon downy mildew resistance gene

[0063] A kind of molecular marker closely linked with melon downy mildew resistance gene specifically comprises the following steps:

[0064] (1) Crossbreed the susceptible farm variety "Karaxai" as the female parent and the disease-resistant resource PI438685 as the male parent to obtain the hybrid F 1 .

[0065] (2) by Hybrid F 1 Self-pollination to obtain F 2 generation group, F 1 Crossed with "Karaxai" to obtain backcross population BC S , F 1 Crossed with PI438685 to obtain backcross population BC r .

[0066] (3) To PI438685, "Karaxai", F 1 、BC S 、BC r and F 2 Identification of downy mildew resistance.

[0067] Preparation of downy mildew inoculum: collect natural early diseased leaves from the field, use a soft brush to pick up the sporangia on the back of the leaves, put them in a beaker filled with sterile water, and inoculate them with the common spot meth...

Embodiment 2

[0081] Embodiment two: the molecular marker SSR-666 closely linked with the melon downy mildew resistance gene 230 Applications

[0082] The invention provides a molecular marker SSR-666 closely linked to the melon downy mildew resistance gene 230 application of the molecular marker SSR-666 230 Be used for the genotype detection of muskmelon variety or line, be used for judging whether this kind or line is resistant to melon downy mildew, comprises the following steps:

[0083] (1) Using the disease-resistant resource PI438685 to cross with other melons and propagate to F 2 generation above.

[0084] (2) Genomic DNA is extracted from the muskmelon individual plant obtained by step (1), and SSR-666 is used to 230 The marker is amplified by PCR to detect whether only a unique 230bp specific band occurs, and if only this specific band occurs, it is predicted that the melon plant is resistant to downy mildew.

Embodiment 3

[0085] Embodiment three: the SSR molecular marker SSR-666 linked with the melon downy mildew resistance gene 230 obtained.

[0086] (1) F 1 , F 1 F 2 , F 1 Hybridized with PI438685 to get BCr, F 1 Cross with "Karaxai" to get BCs. By pair of parents, F 1、 f 2 , BCr, and BCs were artificially inoculated and the incidence of each individual plant was counted: BCs, F 1 and "Karaxai" were all susceptible, PI438685 was resistant, and the ratio of resistant to susceptible in BCr was approximately 1:1, and F 2 The population statistics of generation segregation showed that among the 197 strain populations, 53 strains were disease-resistant and 144 strains were susceptible, and the resistance-sensitivity ratio was 1:3, so it was determined that the downy mildew resistance gene of PI438685 was single-gene recessive.

[0087] (2) Inoculation of downy mildew and disease classification: adopt the method of spraying inoculation with conidial spore suspension and 6-level disease cla...

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Abstract

The invention discloses a molecular marker closely linked with a melon pseudoperonospora cubensis resisting gene and the application thereof. Based on the seedling-stage resource pseudoperonospora cubensis resistance identification and selection principle and the prior art, a melon resource PI438685 with high pseudoperonospora cubensis resistance is selected as the high-resistance resource, a melon pseudoperonospora cubensis anti-fever F2-generation group is established by means of the high-resistance resource PI438685 and the susceptible farm variety Clarke, the molecular marker SSR-666230 linked with the PI438685 pseudoperonospora cubensis resisting gene is found by means of well-known heredity software, and the genetic distance between the marker and the resistance gene is 2.66 cm. By extracting genome DNA of a single melon plant and conducting PCR amplification with the marker SSR-666230, whether a unique 230bp specific band appears is detected, it is predicted that the single melon plant has pseudoperonospora cubensis resistance if yes, the technical effects of shortening breeding period and improving selection accuracy during pseudoperonospora cubensis resisting strain breeding are realized, and practical value is high.

Description

technical field [0001] The invention relates to the field of agricultural biotechnology. Specifically, the invention relates to the technical field of molecular markers closely linked with the new melon downy mildew resistance gene and its application. Background technique [0002] Melon is an important economic crop. The planting area and output of melon in my country rank first in the world. Melon downy mildew [Pseudoperonosporacubensis (Berk.etCurt.) Rostov.] under the conditions of high temperature and high humidity, the epidemic is rapid, the damage is serious, and the control is difficult. It has a direct impact on the yield, fruit sugar content and commercial rate of melon. , is a devastating disease. Chemical control of downy mildew is not only inefficient, but also easy to cause environmental pollution, and cultivating disease-resistant varieties is one of the safest, most effective and cost-saving measures to prevent and control downy mildew in melon. [0003] U...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 张学军李寐华杨永张红张永兵伊鸿平王豪杰
Owner XINJIANG AGRI SCI ACAD CANTALOUPE RES CENT
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