The Application of Photosynthetic Bacteria in the Prevention and Control of Stem Mustard Clubroot and the Control Agent of Stem Mustard Clubroot
A technology for photosynthetic bacteria and tuber mustard, applied in the application, fungicides, biocides and other directions, can solve the problems of unreported application of photosynthetic bacteria, achieve good market application prospects, widen the scope of application, and improve the effect of vegetable yield.
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Embodiment 1
[0016] Embodiment 1 Rhodopseudomonas palustris bacterial strain and cultivation
[0017] Rhodopseudomonas faecalis (Rhodopseudomonas faecalis) No.: 1.2176, purchased from China General Microorganism Culture Collection and Management Center. Rhodopseudomonas palustris culture medium adopts 0925 medium (provided by China General Microorganism Culture Collection Management Center) formula as follows:
[0018] 0925 medium (composition and final concentration): Potassium dihydrogen phosphate 1.00g / L, calcium chloride 0.1g / L, sodium bicarbonate 3.00g / L, sodium acetate 1.00g / L, magnesium chloride 0.5g / L, chloride Ammonium 1.00g / L, Sodium Chloride 1.0g / L, Trace Element Solution 1.00ml / L, Vitamin Solution 1.0ml / L, Sodium Succinate 1.00g / L, Yeast Extract 1.50g / L, Peptone 0.5g / L , pH6.8.
[0019] Among them, the composition and final concentration of the trace element solution are as follows: ferric chloride tetrahydrate 1.8 g / L, cobalt chloride hexahydrate 0.25 g / L, nickel chloride he...
Embodiment 2
[0021] Embodiment 2 inoculation method
[0022] The plots with severe clubroot disease were selected, and Yongan Xiaoye, a cultivar commonly used in Fuling District, was used as the experimental variety. When transplanting, the roots of the mustard seedlings were cut off, and 100 mL of Rhodopseudomonas palustris was suspended. Liquid (1×10 9 cfu mL -1 ) to 500 mL with water and mix well (final concentration 2×10 8 cfu / mL), 60 root-cut seedlings were soaked for 30 min, and the parallel operation experiment was repeated three times. With clean water as the blank control, the plots were randomly arranged, and the management was the same as that of the general field. During the harvest period, the field disease rate, disease index and number of grown vegetables were investigated in the treatment area and the control area, and the control effect of the strains in the treatment area was measured.
Embodiment 3
[0023] Embodiment 3 disease statistics
[0024] 1. Statistical method:
[0025] The severity of clubroot mustard disease is graded as 5 grades, and the standards are (Gao Mingquan, 2012):
[0026] Grade 0: Healthy plants.
[0027] Grade 1: Onset of lateral roots, small and few tumors, white in color, no or slight effect on plant growth, yield loss of 0-5%.
[0028] Grade 2: Tumors are mainly in the middle of the main root, and there are many upper and lower lateral roots, or large and numerous tumors on the lateral roots, which are white or beige, and have certain effects on plant growth, but there are no obvious symptoms in the aerial part, and the yield loss is 6-20%.
[0029] Grade 3: The main root tumor is large and black, the surface is rough and cracked, there are a few lateral roots, the growth of the aboveground part is hindered, the central leaves turn dark green, and they wilt slightly in the sun, and the yield loss is 21-35%.
[0030] Grade 4: The main root tumor...
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