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Production method and use of ergosterol

A technology of ergosterol and silica gel, which is applied in the field of ergosterol preparation, can solve the problems of long preparation time, insufficient mild reaction conditions, and insufficient ergosterol yield, and achieve the effects of simple preparation method, short time consumption and easy realization

Inactive Publication Date: 2016-08-24
周礼红
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the reaction conditions required in the preparation method of the invention are still not gentle enough, and the required preparation time is long, and the output of the selected bacterial strain is still not high enough when preparing ergosterol

Method used

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  • Production method and use of ergosterol
  • Production method and use of ergosterol
  • Production method and use of ergosterol

Examples

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Comparison scheme
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Embodiment 1

[0041] Example 1: A method for preparing ergosterol, including the step of extracting a fermentation sample of Monascus as a raw material.

Embodiment 2

[0042] Example 2: A use of ergosterol, which is used in the preparation of drugs with anti-lipid peroxidation activity.

Embodiment 3

[0043] Example 3: A method for preparing ergosterol, including the following steps: take a dried Monascus fermentation sample, add extraction solvent, extract by extraction to obtain a crude extract, and then use column chromatography to elute the crude extract to obtain one Purify the eluent, perform silica gel thin-layer chromatography on the primary purified eluent, combine the eluents from the same silica gel thin-layer chromatography results and perform column chromatography elution again to obtain the secondary purified eluent. The second purification eluent is subjected to silica gel thin layer chromatography, the eluents of the same silica gel thin layer chromatography result are combined, the solvent is removed, and the ergosterol is recrystallized by recrystallization solvent.

[0044] A use of the above-mentioned ergosterol, which is used in the preparation of drugs with anti-lipid peroxidation activity.

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Abstract

The invention discloses a production method of ergosterol. The production method includes the following steps: adding an extracting solvent to a dried Monascus purpureus-fermented sample, extracting by an extraction process to obtain crude extract, eluting the crude extract by column chromatography to obtain primary purified eluent, subjecting the primary purified elution to silica gel thin-layer chromatography, combining eluents with same silica gel thin-layer chromatography results and performing column chromatography again to obtain secondary purified eluent, removing the solvent from the secondary purified eluent, and recrystallizing through petroleum ether to obtain ergosterol. The preparation of ergosterol from Monascus purpureus as material is provided for the first time, crude extract of the sample is extracted first, and an optimal column chromatography eluent is screened then according to distribution conditions and dissolutions in different solvents, so that ergosterol is isolated and purified effectively. The production method of the ergosterol is simple, easy to implement and low in time consumption, the material is easy to obtain and low in cost, and batch production of ergosterol is achieved.

Description

Technical field [0001] The invention relates to the technical field of sterol compound extraction, in particular to a preparation method and application of ergosterol. Background technique [0002] "Ergosterol", also known as "ergosterol", is one of the steroids. The compound can be used in biochemical research, and part of its role is the same as vitamin D2. Ergosterol is an important part of the cell membrane of microorganisms, and it plays an important role in ensuring the integrity of the cell membrane, the activity of membrane-bound enzymes, the fluidity of the membrane, the viability of the cell, and the transportation of cell material. An intermediate for the production of hormone drugs, which can be used to produce cortisone. Have the effect of vitamin D2. Ergosterol has important physiological functions and is a raw material for food, feed and pharmaceutical industries. [0003] The industrial production of ergosterol has two ways of microbial biosynthesis and chemical...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J9/00A61K31/575A61P39/06
CPCC07J9/00
Inventor 周礼红侯素媛
Owner 周礼红
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