Corynebacterium glutamicum and method for producing high-yield isoleucine with same

Abstract

The invention relates to Corynebacterium glutamicum and a method for producing high-yield isoleucine with the same, and belongs to the field of microorganisms and genomics. The Corynebacterium glutamicum, specifically Corynebacterium glutamicum YI is preserved with a preservation number CGMCC NO. 12153. The Corynebacterium glutamicum is obtained by means of mutagenesis and is fermented in a 30L fermentation cylinder for 32-40 hours; the yield of isoleucine is up to 28g / L; accordingly, the method has the advantages of short time for fermentation and high yield and is superior to the prior art. The invention also discloses a gene mutation site of the strain relative to a standard strain, providing a new direction for correlational researches.

Description

Technical field:

[0001] The invention relates to the fields of microorganisms and genomics, in particular to a Corynebacterium glutamicum and a method for high-yielding L-isoleucine. Background technique:

[0002] Corynebacterium glutamicum is a Gram-positive bacterium of the order Actinomycetes, and its cells are arranged in a short rod shape. Corynebacterium glutamicum plays a pivotal role in the field of amino acid fermentation and has been safely used for nearly 60 years. Currently, most amino acids including L-lysine, L-valine, L-threonine, L-leucine, L-isoleucine, L-alanine, L-aspartic acid, etc. Both began to use Corynebacterium glutamicum fermentation for production.

[0003] The domestic production of L-isoleucine mainly relies on microbial fermentation, and the strains used are mainly Corynebacterium glutamicum. However, the current lack of understanding of the dynamic regulation of its metabolic network limits the progress of further research on the application ...

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